| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,352,254 | 2 bp→GC | intergenic (‑569/‑12) | yhcC ← / → gltB | hypothetical protein/glutamate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,352,254 | 0 | C | G | 99.1% | 296.9 / NA | 113 | intergenic (‑569/‑13) | yhcC/gltB | hypothetical protein/glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (63/49); minor base A (1/0); total (64/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 3,352,255 | 0 | G | C | 99.1% | 292.9 / NA | 112 | intergenic (‑570/‑12) | yhcC/gltB | hypothetical protein/glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (63/48); minor base T (1/0); total (64/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
TTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGA > NC_000913/3352185‑3352322 || ttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcg < 1:766137/76‑1 (MQ=255) tATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgc < 2:74822/76‑1 (MQ=255) tATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgc < 2:2587052/76‑1 (MQ=255) aTGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcg > 1:747639/1‑76 (MQ=255) tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc > 1:2840207/1‑76 (MQ=255) tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc < 1:7061/76‑1 (MQ=255) tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc < 2:3727748/76‑1 (MQ=255) tGCAGTAATTGAGACCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc < 1:99465/76‑1 (MQ=255) cAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGt > 1:1548771/1‑76 (MQ=255) gTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAt > 1:2966983/1‑76 (MQ=255) gTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAt > 1:3297793/1‑76 (MQ=255) gTAAGTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCCAGGCGCGCGTAt > 2:421982/1‑76 (MQ=255) tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc > 2:153482/1‑71 (MQ=255) tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc < 1:153482/71‑1 (MQ=255) tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg < 1:2210890/76‑1 (MQ=255) tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg < 1:1734352/76‑1 (MQ=255) tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg > 1:2494054/1‑76 (MQ=255) tAATTGAGATCCCCGCTTCACCGTATGAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg < 1:1744197/76‑1 (MQ=255) aTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGac > 2:2713498/1‑76 (MQ=255) ttGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca < 2:4401234/76‑1 (MQ=255) gagaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACg < 1:1878751/76‑1 (MQ=255) gagaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACg < 2:1184297/76‑1 (MQ=255) agaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGc < 2:4457493/76‑1 (MQ=255) agaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGc < 1:2714651/76‑1 (MQ=255) gaTCCCCTCTTCACCGTATTAACCGATGCGAAAACGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCa > 1:3003802/1‑76 (MQ=255) gaTCCCATCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCa > 1:4052545/1‑76 (MQ=255) tCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaaa > 1:1480875/1‑76 (MQ=255) tCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaaa < 2:3024632/76‑1 (MQ=255) ccccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAc < 2:4786448/76‑1 (MQ=255) cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcc > 1:1882887/1‑76 (MQ=255) ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc > 2:1998361/1‑76 (MQ=255) ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc > 1:1687113/1‑76 (MQ=255) ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc < 1:3749367/76‑1 (MQ=255) ctctTGACCGTGTGGACCGATGCGAAAAGGACAACAAGGGGGCGAATGGGAGGCGCGAGTATGACACGCAAAcccc < 2:2269311/76‑1 (MQ=255) ctctTCACCGTATTAGCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcccc < 2:3971576/76‑1 (MQ=255) tctTCACCGTATTAACCGATGCGAAAAGGACACCCAGGGGGCGAATGCGAGGCGCGCGTATGACCGGCAACCCCCg > 2:243239/1‑76 (MQ=255) tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCg > 1:1248446/1‑76 (MQ=255) tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCg > 1:2205115/1‑76 (MQ=255) tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATACGAGGCGCGCGTATGACACGCAAACCCCg > 1:128421/1‑76 (MQ=255) ctTCACCGTAGTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGt < 2:4291953/76‑1 (MQ=255) ttCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTc < 1:350733/76‑1 (MQ=255) tCACCGTATTAACCGATGCGAACAGGACAACAAGGGGGACCATGCGAGGCGCGCGTCTGACACGCAACCCCCgcac > 1:374460/1‑73 (MQ=255) tCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcccc > 1:4800395/1‑72 (MQ=255) tCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcccc < 2:4800395/72‑1 (MQ=255) cACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAAGCGAGGCGCGCGTATGACACGCAAACCCcgccgc > 2:4702400/1‑76 (MQ=255) aCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAGGCGAGGCGCGCGTCTGACACGCAAACCCCGTCGcc > 2:4229725/1‑76 (MQ=255) ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCa > 1:4129523/1‑76 (MQ=255) tgTATTAACCGATGCGAAAAGGACAACATGGAGGCCTATGTGAAGAGCGAGTATGGCTCGCACACCACGTTGCCAc > 1:618657/2‑76 (MQ=255) cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCCAACCCCGTCGCCAc > 2:1538385/1‑76 (MQ=255) cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAc < 2:4467751/76‑1 (MQ=255) cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAc < 1:4686308/76‑1 (MQ=255) cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAc > 1:2266570/1‑76 (MQ=255) gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAcc > 2:3270444/1‑75 (MQ=255) gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACg < 1:2551507/76‑1 (MQ=255) tAACCGATGCGAGAAGGACAACAAGGGGGCGAATGCGAGGGGCGGGTATGACACGCAAACCGCGTCGCCACGctct < 2:3365783/76‑1 (MQ=255) aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCCAACCCCGTCGCCACGCTCtt < 2:3003802/76‑1 (MQ=255) aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCtt > 1:1298752/1‑76 (MQ=255) aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGCCCCCACGCTCtt > 2:4506631/1‑76 (MQ=255) aCCGATGCGAAAAGGACAACAAGTGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCttt < 2:4129523/76‑1 (MQ=255) aCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCttt > 2:3433510/1‑76 (MQ=255) cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt > 2:743958/1‑76 (MQ=255) cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt > 1:4668512/1‑76 (MQ=255) cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt > 2:3283920/1‑76 (MQ=255) cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt < 1:3805660/76‑1 (MQ=255) gttGCGAAAAGGCCAGCAAGGGGGCGAAGGCGCGGCGGGCGTATGTCACGCCCACCCCGTCGCCCCGCTCTTtgtg < 2:3872598/74‑1 (MQ=255) gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACCCGCAAACCCCGCCCCCCCGCCCTTTCtg > 2:174427/1‑76 (MQ=255) gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg > 1:3456545/1‑76 (MQ=255) gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCCTTCtg > 1:3255331/1‑76 (MQ=255) gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTAGCCACGCTCTTTCtc > 1:3164050/1‑75 (MQ=255) aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt < 1:940400/76‑1 (MQ=255) aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt < 2:1935613/76‑1 (MQ=255) aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTCTCtgt > 2:2572257/1‑76 (MQ=255) aTGCGAAAAAGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt < 2:4756455/76‑1 (MQ=255) aaaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg < 1:3585955/76‑1 (MQ=255) aaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt > 2:2170716/1‑76 (MQ=255) aaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt < 1:2150785/76‑1 (MQ=255) aaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTg > 2:2284975/1‑76 (MQ=255) aaGGACAACAAGGGGGCGAAGGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGACCgcg > 2:286487/1‑74 (MQ=255) aGGACAACACGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgc > 1:415247/1‑76 (MQ=255) aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgc < 2:1176706/76‑1 (MQ=255) aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgc > 1:3708601/1‑76 (MQ=255) gACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc > 1:2136825/1‑76 (MQ=255) acaacaAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCgcgcgcc > 1:24456/1‑75 (MQ=255) caacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGCCGCCACGCTCTTTCTGCGCCCGTGCGCAg > 2:3865583/1‑76 (MQ=255) aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc < 2:165213/73‑1 (MQ=255) aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAga < 2:3029478/76‑2 (MQ=255) aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc > 1:599424/1‑76 (MQ=255) aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc > 1:1137070/1‑76 (MQ=255) aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc > 2:678567/1‑76 (MQ=255) acaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCg > 1:3753082/1‑76 (MQ=255) acaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCg > 1:3219566/1‑76 (MQ=255) caaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg > 1:4123962/1‑76 (MQ=255) caaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg < 1:1733000/76‑1 (MQ=255) caaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg > 1:3536699/1‑76 (MQ=255) aaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGt < 2:4259599/76‑1 (MQ=255) aGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGtt < 1:2353218/76‑1 (MQ=255) gggggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc > 2:3167762/1‑76 (MQ=255) ggCGAATGCGAGGCGCGCGTATGGCACGCAAGCCCCGGCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa < 2:3419186/76‑1 (MQ=255) ggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa < 1:1297241/76‑1 (MQ=255) ggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa > 2:2116408/1‑76 (MQ=255) || TTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGA > NC_000913/3352185‑3352322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |