| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,831:1 | +G | 100% | pseudogene (251/261 nt) pseudogene (251/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,831 | 1 | . | G | 100.0% | 77.6 / NA | 23 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (12/11); total (12/11) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAC > W3110S.gb/547766‑547893 | cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gggg > 1:1211351/1‑70 (MQ=255) gcgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg < 1:966342/71‑1 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggggg < 1:1665809/59‑2 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCa > 1:203340/1‑69 (MQ=255) aaGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGt < 1:49411/71‑1 (MQ=255) gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa > 1:1268805/1‑70 (MQ=255) gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgt < 1:1289875/71‑1 (MQ=255) aaGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc > 1:1238664/1‑71 (MQ=255) aaGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc < 1:72438/71‑1 (MQ=255) aGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg > 1:1426117/1‑71 (MQ=255) aGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg > 1:886588/1‑71 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt < 1:1967634/68‑1 (MQ=255) gcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATg > 1:1125810/1‑62 (MQ=255) ggCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc < 1:347640/63‑1 (MQ=255) cAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa > 1:364006/1‑48 (MQ=255) cAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa > 1:80303/1‑48 (MQ=255) aaTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgt > 1:976101/1‑45 (MQ=255) aTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAc < 1:83867/39‑1 (MQ=38) aCTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc > 1:712895/1‑49 (MQ=255) aCTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa > 1:725283/1‑59 (MQ=255) atGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc < 1:443082/70‑1 (MQ=255) atGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc < 1:440611/70‑1 (MQ=255) tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc < 1:945491/47‑1 (MQ=255) gAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg < 1:838387/49‑1 (MQ=255) gAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg > 1:848476/1‑49 (MQ=255) | CGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAC > W3110S.gb/547766‑547893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |