New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 4125178148 (6.500)5 (0.250) 4/90 NT 3.5% coding (674/837 nt) dam DNA adenine methylase
?W3110S.gb = 4125213 145 (7.210)coding (709/837 nt) dam DNA adenine methylase
Rejected: Frequency below/above cutoff threshold.

GTTTTACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCTGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4125103‑4125178
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctgat‑ACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACCTTC  <  W3110S.gb/4125213‑4125144
                                                                                                                                                   
GTTTTACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTG                                                                              <  1:1961521/71‑1
GTTTTACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTG                                                                              <  1:186660/71‑1
GTTTTACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTG                                                                              <  1:1637522/71‑1
 TTTTACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGC                                                                             >  1:434641/1‑71
 TTTTACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGC                                                                             <  1:522330/71‑1
   TTACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCT                                                                            >  1:1746361/1‑70
    TACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCTG                                                                           <  1:1621620/70‑1
            AACAACAAGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCTGAT                                                                         >  1:1265737/1‑64
                   AGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTG                                                                              <  1:452109/52‑1
                    GCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCTGATACCACTCACGCGTT                                                          >  1:1772951/1‑70
                        ATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCTG                                                                           <  1:1556308/50‑1
                           TGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCT                                                                            >  1:843874/1‑46
                           TGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCT                                                                            >  1:846293/1‑46
                                     CGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCTGATCTCCAATCACGCGTTAACATCGTAT                                                >  1:1137404/1‑64
                                     CGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCTGA                                                                          <  1:349595/38‑1
                                         GAAGGTCTGGTTGAGCGCCATATTCCAGTGCTGATACCACTCACGCGTTAACATCGTATCGTGATTG                                        >  1:1906038/1‑67
                                          AAGGTCTGGTTGAGCGCCATATTCCAGTGCTGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGAT                                   <  1:2039804/71‑1
                                                     GAGCGCCATATTCCAGTGCTGATCTCCACTCACGCGTTAACATCGTATCGTGATT                                         <  1:1406212/55‑1
                                                                      GCTGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGA       >  1:1502263/1‑71
                                                                      GCTGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGA       >  1:465990/1‑71
                                                                      GCTGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGA       >  1:2075499/1‑71
                                                                      GCTGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGA       >  1:511557/1‑71
                                                                      GCTGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGG                   >  1:2007184/1‑59
                                                                       CTGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGA                                    <  1:2071654/41‑1
                                                                        TGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACC     >  1:841563/1‑71
                                                                        TGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACC     >  1:284079/1‑71
                                                                        TGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACC     >  1:605391/1‑71
                                                                        TGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATAT                     <  1:69297/55‑1
                                                                        TGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACT                            <  1:1149727/48‑1
                                                                        TGATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACT                            <  1:1715872/48‑1
                                                                         GATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACCT    >  1:776540/1‑71
                                                                         GATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATA                      >  1:375586/1‑53
                                                                          ATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACCTT   >  1:975721/1‑71
                                                                          ATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACCT    <  1:705971/70‑1
                                                                          ATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACC     <  1:1964366/69‑1
                                                                          ATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAA            >  1:1403287/1‑62
                                                                          ATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGC                >  1:1162895/1‑58
                                                                          ATACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGG                   <  1:384606/55‑1
                                                                          ATACCACTCACGCGGTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACCTT   >  1:1964992/1‑71
                                                                           TACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACCTTC  <  1:1780349/71‑1
                                                                                                                                                   
GTTTTACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCGAAGGTCTGGTTGAGCGCCATATTCCAGTGCTGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4125103‑4125178
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctgat‑ACCACTCACGCGTTAACATCGTATCGTGATTGGAGATCAGCACTGGAATATGGCGCTCAACCAGACCTTC  <  W3110S.gb/4125213‑4125144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.