| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 155,463 | A→T | 100% | *247K (TAA→AAA) | ecpD ← | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 155,463 | 0 | A | T | 100.0% | 108.4 / NA | 36 | *247K (TAA→AAA) | ecpD | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (17/19); total (17/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAA > W3110S.gb/155396‑155530 | gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg > 1:2001070/1‑71 (MQ=255)gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg > 1:1932264/1‑71 (MQ=255) tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc < 1:1442288/71‑1 (MQ=255) aTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa > 1:938110/1‑71 (MQ=255) atatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGc < 1:231630/61‑1 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:701021/53‑1 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:1997191/1‑71 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa < 1:731367/71‑1 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:1309162/71‑1 (MQ=255) tCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg < 1:1732424/49‑1 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCaa < 1:1907929/71‑1 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt < 1:295570/44‑1 (MQ=255) gCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGc < 1:708551/41‑1 (MQ=255) gCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt < 1:1571419/43‑1 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTa > 1:2070880/1‑44 (MQ=255) tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAAt > 1:1240499/1‑71 (MQ=255) ccTGCGGAATTATATCCTTGCCTTATTTCAGCCTGGCATTAc > 1:842595/1‑42 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAAt < 1:443647/70‑1 (MQ=255) cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCg > 1:936642/1‑70 (MQ=255) ggAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa > 1:1444646/1‑60 (MQ=255) aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa < 1:308144/71‑1 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg > 1:128575/1‑71 (MQ=255) tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg < 1:1997514/70‑1 (MQ=255) ccTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACAAAAGTCATTAATgg > 1:591681/1‑57 (MQ=255) gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACttt < 1:1596287/69‑1 (MQ=255) gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg > 1:1784957/1‑71 (MQ=255) ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt < 1:1418638/46‑1 (MQ=255) cTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg < 1:373359/61‑1 (MQ=255) gATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAg > 1:1408443/1‑70 (MQ=255) gATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAg > 1:370654/1‑70 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa < 1:1967245/41‑1 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa > 1:802111/1‑45 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCg > 1:1361176/1‑50 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAg < 1:386/69‑1 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGaa > 1:2012262/1‑71 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGaa < 1:1088920/71‑1 (MQ=255) | GATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAA > W3110S.gb/155396‑155530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |