| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 219,687 | A→T | 14.3% | A103A (GCT→GCA) | rcsF ← | predicted outer membrane protein, signal |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 219,687 | 0 | A | T | 14.3% | 104.7 / 11.0 | 42 | A103A (GCT→GCA) | rcsF | predicted outer membrane protein, signal |
| Reads supporting (aligned to +/- strand): ref base A (15/21); new base T (4/2); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01 | |||||||||||
CCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAG > W3110S.gb/219619‑219756 | ccGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAg > 1:687570/1‑70 (MQ=255) tacatacaGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTg < 1:1525268/71‑1 (MQ=255) acatacaGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTgg > 1:124040/1‑71 (MQ=255) catacaGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGc < 1:1575598/71‑1 (MQ=255) atacaGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGc < 1:1790827/64‑1 (MQ=255) caGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCttt > 1:1797206/1‑70 (MQ=255) cGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTaga < 1:1047962/71‑1 (MQ=255) cGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTa > 1:1730168/1‑69 (MQ=255) gATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTagag > 1:955123/1‑71 (MQ=255) ccTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAt < 1:231563/71‑1 (MQ=255) cTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAtt < 1:280759/71‑1 (MQ=255) tGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTc > 1:2091035/1‑52 (MQ=255) tGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAttt > 1:847256/1‑71 (MQ=255) gCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTg > 1:420453/1‑70 (MQ=255) tACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTaga < 1:194655/55‑1 (MQ=255) aCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGtt < 1:339947/60‑1 (MQ=255) aCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATcc > 1:1957774/1‑71 (MQ=255) cTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCtt < 1:768973/71‑1 (MQ=255) tGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGc < 1:1203444/37‑1 (MQ=255) aCTTCGCTGCTATGCAGTAATACAGCATTGGCTTTCAt > 1:1651401/1‑38 (MQ=255) cTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCg < 1:180920/71‑1 (MQ=255) cGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGt < 1:2010876/70‑1 (MQ=255) gCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTg < 1:975730/71‑1 (MQ=255) cAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTgg < 1:83197/71‑1 (MQ=255) cAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTgg < 1:1012874/71‑1 (MQ=255) aGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTgg > 1:1309891/1‑70 (MQ=255) gCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAttt < 1:1036433/47‑1 (MQ=255) caaTGCTGTATTACTGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGaa < 1:1627525/68‑1 (MQ=255) caaTGCTGTATTACTGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGaa < 1:1164331/68‑1 (MQ=255) tATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCt < 1:1660435/54‑1 (MQ=255) tGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGc < 1:96515/70‑1 (MQ=255) aGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAt < 1:1279883/38‑1 (MQ=255) aGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAAt > 1:1410412/1‑65 (MQ=255) tattACTGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGc > 1:2074263/4‑65 (MQ=255) tattACTGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGc > 1:1915067/4‑65 (MQ=255) tattACTGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGc > 1:177740/4‑65 (MQ=255) tAATACTGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGc > 1:1088587/1‑65 (MQ=255) tAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCgg < 1:957603/56‑1 (MQ=255) tAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcggcg < 1:779333/71‑1 (MQ=255) aTACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATc > 1:697683/1‑41 (MQ=255) cAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGt > 1:1464904/1‑47 (MQ=255) cAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGagag > 1:915768/1‑71 (MQ=255) | CCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAG > W3110S.gb/219619‑219756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |