| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,184,032 | G→A | 16.7% | Y125Y (TAC→TAT) | potD ← | polyamine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,184,032 | 0 | G | A | 16.7% | 70.7 / 7.4 | 30 | Y125Y (TAC→TAT) | potD | polyamine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/10); new base A (3/2); total (18/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
CAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTC > W3110S.gb/1183965‑1184101 | cAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGagtag < 1:1439096/71‑1 (MQ=255) aGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGagtagt > 1:2071992/1‑71 (MQ=255) tCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGtt < 1:1987577/70‑1 (MQ=255) cGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTa < 1:514661/70‑1 (MQ=255) ggATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTAt < 1:1911361/70‑1 (MQ=255) gCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCaa > 1:1164128/1‑70 (MQ=255) tAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGagtagt > 1:730424/1‑45 (MQ=255) aaCACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCttgttg < 1:1576029/70‑1 (MQ=255) acacCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGa < 1:1939907/70‑1 (MQ=255) cacCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAg > 1:384694/1‑70 (MQ=255) acCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCaaa > 1:323825/1‑57 (MQ=255) cAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAg > 1:1125406/1‑67 (MQ=255) cAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGc > 1:1373431/1‑68 (MQ=255) aaTCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCaaaa > 1:808444/1‑55 (MQ=255) aaTCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGt > 1:1870619/1‑71 (MQ=255) gTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGAt > 1:1831508/1‑70 (MQ=255) gTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATc > 1:1151389/1‑71 (MQ=255) cGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCttgtt > 1:824970/1‑54 (MQ=255) cACCCCAGATATACGGAATGGAATAGTCGTTATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCgagg < 1:248305/71‑2 (MQ=255) cACCCCAGATATACGGAATGGAATAGTCGTTATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCgagg < 1:1255324/71‑2 (MQ=255) ccccAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCttgttg > 1:562421/1‑51 (MQ=255) ccccAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGAt > 1:493430/1‑64 (MQ=255) cccAGATATACGGAATGGAGTAGTCGTTATTCGGGTCaaaa < 1:576123/41‑1 (MQ=255) cAGATATACGGAATGGAATAGTCGTTATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGc > 1:749794/1‑70 (MQ=255) cAGATATACGGAATGGAATAGTCGTTATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGc > 1:136346/1‑70 (MQ=255) cAGATATACGGAATGGAATAGTCGTTATTCGGGTCAAACGGCTTGTTGAGAATGTCTGGATCGAGGTTGc > 1:273234/1‑70 (MQ=255) ataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCt > 1:237988/1‑52 (MQ=255) taCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCAt < 1:745859/47‑1 (MQ=255) taCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt > 1:1427228/1‑71 (MQ=255) cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTg < 1:838052/71‑1 (MQ=255) cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTg < 1:863148/71‑1 (MQ=255) aaTGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTa < 1:2035679/71‑1 (MQ=255) gAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGaa > 1:705753/1‑59 (MQ=255) gtagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTc > 1:1334836/1‑70 (MQ=255) | CAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTC > W3110S.gb/1183965‑1184101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |