| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,740 | T→G | 85.3% | G118G (GGT→GGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,740 | 0 | T | G | 85.3% | 231.5 / 11.8 | 73 | G118G (GGT→GGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (5/6); new base G (33/29); total (38/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCG > W3110S.gb/70673‑70807 | tttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttc > 1:1119393/1‑71 (MQ=255) ttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct > 1:1863115/1‑71 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:1225137/70‑1 (MQ=255) aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt < 1:1455004/70‑1 (MQ=255) aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt < 1:1933269/70‑1 (MQ=255) tCGTCCGCGCTCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc > 1:130968/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc > 1:1697073/1‑71 (MQ=255) cGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGccc > 1:1808469/1‑71 (MQ=255) cGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc < 1:1359454/70‑1 (MQ=255) gTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCg < 1:758690/71‑1 (MQ=255) ccGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa > 1:1495476/1‑71 (MQ=255) cgcgcgCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGAt < 1:1471589/71‑1 (MQ=255) cgcgcgCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGAt < 1:820109/71‑1 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg < 1:614018/71‑1 (MQ=255) gcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg > 1:850779/1‑69 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgc > 1:372759/1‑71 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTGCTTTCGCCCGGATGAAg < 1:978249/70‑1 (MQ=255) ccTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcg < 1:1197375/71‑1 (MQ=255) ccTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg < 1:58664/71‑1 (MQ=255) ccTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg < 1:1856362/71‑1 (MQ=255) ccTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgc < 1:945075/70‑1 (MQ=255) tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCg < 1:2097786/60‑1 (MQ=255) tACTGGCATGAATGGCTTAACTGGCAGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa > 1:47265/1‑71 (MQ=255) aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc > 1:344180/1‑71 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttcttt > 1:1018475/1‑49 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGc > 1:277545/1‑71 (MQ=255) gCATGAATCGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAg > 1:1633075/1‑62 (MQ=255) cATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa < 1:1866844/65‑1 (MQ=255) aTGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGcc < 1:1016191/70‑1 (MQ=255) aTGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCg < 1:383983/71‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCg < 1:491182/52‑1 (MQ=255) gAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa > 1:2041274/1‑53 (MQ=255) gAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc > 1:1497593/1‑70 (MQ=255) gAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCa > 1:864273/1‑71 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa > 1:799992/1‑50 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:1092583/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:202183/1‑71 (MQ=255) tGGCTTAACTGGCCGTCAAAATTTGACAATACGGGGTTCTTTCGCCCGGATGAAGCGCa > 1:317137/1‑59 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:1661014/1‑69 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:476815/1‑69 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc < 1:988452/70‑1 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa < 1:1316594/71‑1 (MQ=255) cTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAg > 1:670018/1‑71 (MQ=255) cTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAg > 1:6119/1‑71 (MQ=255) tAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGaa < 1:806800/49‑1 (MQ=255) tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGa < 1:219472/48‑1 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg < 1:1289010/70‑1 (MQ=255) aCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcgc < 1:384250/51‑1 (MQ=255) tGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg < 1:737126/48‑1 (MQ=255) ggCCGTCAATATTTGCCAATACGGGGTTCTTTCGccc > 1:1210431/1‑37 (MQ=255) gCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt < 1:2008905/71‑1 (MQ=255) gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa > 1:209563/1‑39 (MQ=255) gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt < 1:438949/71‑1 (MQ=255) gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt < 1:441374/71‑1 (MQ=255) ccGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:288346/71‑1 (MQ=255) ccGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:881896/71‑1 (MQ=255) gTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGaa > 1:392928/1‑40 (MQ=255) gTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCg < 1:205823/52‑1 (MQ=255) tCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTgg > 1:1951459/1‑71 (MQ=255) aaTATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTggg > 1:1950596/1‑70 (MQ=255) aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt > 1:1883098/1‑67 (MQ=255) atatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa > 1:127030/1‑55 (MQ=255) tatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc > 1:1207310/1‑53 (MQ=255) ttttCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa > 1:1488679/1‑38 (MQ=38) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc > 1:1187469/1‑39 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCGGCCGCATTTCAGc > 1:1573744/1‑54 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGc > 1:1399574/1‑43 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt > 1:1435269/1‑62 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaaa < 1:99875/70‑1 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt > 1:717235/1‑71 (MQ=255) tGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCa > 1:1767558/1‑71 (MQ=255) tGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGcc > 1:197763/1‑42 (MQ=255) gCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCa < 1:1426746/70‑1 (MQ=255) ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAt > 1:1719653/1‑70 (MQ=255) cAATACGTGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTa < 1:1967486/71‑1 (MQ=255) aaTACGGGGTTCTTTCGCCCGGATGAAGCGCACCa < 1:290680/35‑1 (MQ=255) aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCg > 1:962830/1‑38 (MQ=255) tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:2049864/42‑1 (MQ=255) cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc < 1:926506/71‑1 (MQ=255) ggggTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCg > 1:1109855/1‑71 (MQ=255) tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:108298/49‑1 (MQ=255) | TTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCG > W3110S.gb/70673‑70807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |