BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,563,589	T→A	100%	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,563,589	0	T	A	100.0%	87.1 / NA	29	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (11/18); total (11/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCA  >  W3110S.gb/4563523‑4563648

gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                          >  1:294758/1‑70 (MQ=255)

 tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                        <  1:279512/71‑1 (MQ=255)

      agcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTc                                                   >  1:1783469/1‑71 (MQ=255)

       gcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCg                                                  >  1:911697/1‑71 (MQ=255)

        cagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGc                                                 >  1:1735583/1‑71 (MQ=255)

           cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                              <  1:1799207/71‑1 (MQ=255)

             tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                            >  1:1994854/1‑71 (MQ=255)

             tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                            >  1:1626630/1‑71 (MQ=255)

                tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                         <  1:1051366/55‑1 (MQ=255)

                  aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                         <  1:456476/69‑1 (MQ=255)

                  aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                       >  1:635387/1‑71 (MQ=255)

                     ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGACCATGTGGAacac                                    >  1:1052783/1‑71 (MQ=255)

                     ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGACCATGTGGAacac                                    >  1:1055256/1‑71 (MQ=255)

                       cacaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                  <  1:1255052/71‑1 (MQ=255)

                        acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                 <  1:734054/71‑1 (MQ=255)

                         caTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                          >  1:879205/1‑45 (MQ=255)

                           tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                         <  1:691969/60‑1 (MQ=255)

                                gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                          <  1:1891470/38‑1 (MQ=255)

                                gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg                         <  1:1484497/71‑1 (MQ=255)

                                   ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGt                       <  1:1063112/70‑1 (MQ=255)

                                   ttCCAGCAGGTCATAAATAGGCTGTAACGCAATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                      >  1:1267304/1‑71 (MQ=255)

                                    tCCAGCAGGTCATAAATAGGCTTTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                      <  1:1990658/70‑1 (MQ=255)

                                      cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                 <  1:681916/57‑1 (MQ=255)

                                       agcagGTCATAAATAGGCTGTAACGCCATTTTACt                                                      <  1:2052912/35‑1 (MQ=255)

                                        gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg                 >  1:235163/1‑71 (MQ=255)

                                               aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATTTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa          <  1:831201/71‑1 (MQ=255)

                                               aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa          <  1:1190199/71‑1 (MQ=255)

                                               aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa          <  1:864229/71‑1 (MQ=255)

                                                tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaa         <  1:1570629/71‑1 (MQ=255)

                                                  aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa        <  1:223987/70‑1 (MQ=255)

                                                    tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg                         >  1:476470/1‑51 (MQ=255)

                                                       gCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCa  <  1:1137827/71‑1 (MQ=255)

                                                             aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg              <  1:1959279/53‑1 (MQ=255)

GTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCA  >  W3110S.gb/4563523‑4563648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`