| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,175 | A→G | 82.4% | R263R (CGA→CGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,175 | 0 | A | G | 82.4% | 176.7 / 28.4 | 74 | R263R (CGA→CGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (8/5); new base G (30/31); total (38/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01 | |||||||||||
GCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAAT > W3110S.gb/71105‑71241 | gCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGa < 1:1815916/71‑1 (MQ=255) cTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTa > 1:450453/1‑71 (MQ=255) aCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAtt > 1:358461/1‑71 (MQ=255) cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTAttt < 1:1897393/71‑1 (MQ=255) ccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAtt > 1:555337/1‑69 (MQ=255) ccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTa < 1:239753/71‑1 (MQ=255) ggATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa > 1:376259/1‑70 (MQ=255) ggATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa > 1:1177035/1‑70 (MQ=255) ggATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaa > 1:69064/1‑70 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:1902630/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa < 1:1306988/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa < 1:540607/71‑1 (MQ=255) tGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:1906604/1‑71 (MQ=255) ccTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:174777/67‑1 (MQ=255) ccTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAAt < 1:831059/70‑1 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATg > 1:1714721/1‑70 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:1692178/1‑71 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:2006375/1‑71 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:388667/1‑71 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCa > 1:1869750/1‑70 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc < 1:1643373/70‑1 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCAcc < 1:1219658/71‑1 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTAGCGGGTATTTaaaaaa > 1:1289806/1‑63 (MQ=255) ccACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCggg < 1:1825424/71‑1 (MQ=255) cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgggg > 1:269532/1‑71 (MQ=255) cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGCTATTTa < 1:269792/56‑1 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa > 1:977052/1‑58 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGc > 1:313702/1‑71 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAtt > 1:1350398/1‑52 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:228606/1‑64 (MQ=255) cGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGc > 1:245249/1‑69 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAg > 1:630529/1‑71 (MQ=255) tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGc < 1:1094223/71‑1 (MQ=255) tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGc < 1:1934411/71‑1 (MQ=255) tcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa < 1:1710045/49‑1 (MQ=255) tcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc < 1:2056138/58‑1 (MQ=255) tcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcg < 1:483256/70‑1 (MQ=255) cgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg < 1:1276120/58‑1 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg < 1:489596/57‑1 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag < 1:687198/70‑1 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcg < 1:1693745/71‑1 (MQ=255) aaTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:1743920/1‑48 (MQ=255) aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTa > 1:311731/1‑42 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcgag < 1:1573475/70‑1 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcg > 1:810371/1‑64 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt < 1:785870/71‑1 (MQ=255) tGTAGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg > 1:401655/1‑53 (MQ=39) gTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTa < 1:853212/40‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa < 1:768900/43‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc < 1:1222028/71‑2 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc > 1:1458797/1‑70 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTa > 1:1935980/1‑39 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgggg > 1:459373/1‑52 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCa > 1:1225591/1‑55 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCg > 1:1302041/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgg > 1:1902921/1‑46 (MQ=255) cGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt < 1:706791/71‑1 (MQ=255) cGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt > 1:317255/1‑71 (MQ=255) cGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGtt > 1:1428177/1‑71 (MQ=255) ataTCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGtt < 1:1951380/69‑1 (MQ=255) gATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCggg < 1:1096505/42‑1 (MQ=255) tCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGc > 1:1757723/1‑63 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:1553699/1‑35 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCggg > 1:933781/1‑39 (MQ=255) aaCTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTga < 1:1596120/70‑1 (MQ=255) ctctATTTCTCGCGGGTATTTAAAAAATGCACCggg < 1:1862314/36‑1 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGt > 1:1355350/1‑54 (MQ=39) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa < 1:244994/69‑1 (MQ=255) tATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa > 1:1631793/1‑70 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg < 1:730556/71‑1 (MQ=255) ctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGATCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTg < 1:1731177/70‑1 (MQ=255) ctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTg < 1:618662/70‑1 (MQ=255) cgcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat > 1:924580/1‑71 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGa < 1:1656277/68‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:1436858/70‑1 (MQ=255) | GCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAAT > W3110S.gb/71105‑71241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |