| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 234,956 | Δ1 bp | 5.4% | coding (141/723 nt) | yafS → | predicted S‑adenosyl‑L‑methionine‑dependent methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 234,956 | 0 | G | . | 5.4% | 243.4 / 4.9 | 74 | coding (141/723 nt) | yafS | predicted S‑adenosyl‑L‑methionine‑dependent methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (27/43); new base . (2/2); total (29/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
TATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGC > W3110S.gb/234888‑235024 | tATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAg < 1:7699491/69‑1 (MQ=255)tATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAt > 1:7533452/1‑71 (MQ=255) aTCGCAAGGCGCTGTAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAt < 1:2180261/70‑1 (MQ=255) aTCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAt < 1:3499427/70‑1 (MQ=255) tCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGa > 1:3428717/1‑68 (MQ=255) tCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAtt < 1:7362855/70‑1 (MQ=255) gcgcTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAt‑tt < 1:1095747/71‑1 (MQ=255) gcgcTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAt‑tt < 1:4198614/71‑1 (MQ=255) gcgcTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAt‑tt < 1:197472/71‑1 (MQ=255) cgcTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAt‑tt > 1:7646016/1‑70 (MQ=255) cTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAg < 1:4174467/71‑1 (MQ=255) gAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAgcg > 1:4546391/1‑70 (MQ=255) gcCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGa > 1:2602033/1‑70 (MQ=255) gcCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAA‑ATTGCCAATCTTAAgca < 1:4267613/66‑2 (MQ=25) gcCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAA‑ATTGCCAATCTTAAgca < 1:2630458/66‑2 (MQ=25) cAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTaa < 1:4102394/61‑1 (MQ=255) cAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAA‑ATTGCCAATCTTAAgcaga > 1:1477441/1‑63 (MQ=25) aGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAAt > 1:159085/1‑70 (MQ=255) aGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAAt > 1:2175729/1‑70 (MQ=255) aGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATc < 1:6604795/71‑1 (MQ=255) aGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATc < 1:3815251/71‑1 (MQ=255) cTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCaa < 1:6084786/71‑1 (MQ=255) tCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAtt < 1:7363820/47‑1 (MQ=255) tCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAtt < 1:3599552/47‑1 (MQ=255) tCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAtt < 1:5170112/47‑1 (MQ=255) cAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAtt < 1:6707085/46‑1 (MQ=255) cAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAtt < 1:2575983/46‑1 (MQ=255) aaCCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAAtt < 1:1257238/70‑1 (MQ=255) aaCCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAA‑ATTGCCAATCTTAAg > 1:1039156/1‑56 (MQ=25) aCCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGc > 1:3403722/1‑71 (MQ=255) cccGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGa > 1:3877471/1‑60 (MQ=255) cGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAt‑tt < 1:2182094/49‑1 (MQ=255) cGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAt‑tt < 1:1770795/49‑1 (MQ=255) cACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTgc > 1:1117493/1‑70 (MQ=255) aCTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTgcgc > 1:2845840/1‑71 (MQ=255) aCTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTgcg < 1:6573259/70‑1 (MQ=255) caaaaaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAgcg < 1:1556211/43‑1 (MQ=255) cTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAgcg < 1:981604/45‑1 (MQ=255) cTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGaa > 1:3315049/1‑50 (MQ=255) cTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGcgcg < 1:5806499/71‑1 (MQ=255) tAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGaa < 1:3231021/49‑1 (MQ=255) tAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGaa < 1:3025879/49‑1 (MQ=255) tAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAAt < 1:2390648/51‑1 (MQ=255) tAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCgg > 1:1277035/1‑71 (MQ=255) aaaaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAAtt > 1:5899163/1‑55 (MQ=255) aaaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCgg < 1:6920621/69‑1 (MQ=255) aaaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGtt > 1:2082765/1‑71 (MQ=255) aaaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGt < 1:7321672/70‑1 (MQ=255) aaaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGt < 1:672675/70‑1 (MQ=255) aaaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGt < 1:2418330/70‑1 (MQ=255) aaaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGt < 1:2444846/70‑1 (MQ=255) aaaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGt < 1:2495726/70‑1 (MQ=255) aaaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGAGAAGCGTGCGCGGtt > 1:5911194/1‑71 (MQ=255) aaTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGttt < 1:3468013/71‑1 (MQ=255) aTGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtc < 1:5544257/71‑1 (MQ=255) tGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtct > 1:4208070/1‑71 (MQ=255) tATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtctc < 1:3383255/70‑1 (MQ=255) tGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCtcatc < 1:5336890/71‑1 (MQ=255) tGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCtcat > 1:5426026/1‑70 (MQ=255) ggTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtctc > 1:5845911/1‑67 (MQ=255) tttCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAAt < 1:2160281/38‑1 (MQ=255) tttCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGa > 1:1773583/1‑47 (MQ=255) tttCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGa > 1:748859/1‑47 (MQ=255) tCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTg < 1:4484582/70‑1 (MQ=255) cATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCGTCAAGTGaa > 1:3154912/1‑71 (MQ=255) aTCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGttt > 1:129530/1‑57 (MQ=255) aTCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCtca > 1:4718575/1‑61 (MQ=255) tCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGaa < 1:6762619/43‑1 (MQ=255) tCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGa < 1:7375454/42‑1 (MQ=255) tCTGATTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg > 1:5529879/1‑71 (MQ=255) cTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGt < 1:2626952/68‑1 (MQ=255) cTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGt < 1:1523903/68‑1 (MQ=255) cTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTc < 1:327880/71‑1 (MQ=255) tAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTg > 1:7262553/1‑71 (MQ=255) aaGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTgc > 1:35165/1‑71 (MQ=255) | TATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAT‑TTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGC > W3110S.gb/234888‑235024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |