Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 2176983–2178180 2178181 2–1199 156 [0] [0] 158 [gatA]–[gatA] [gatA],insH,[gatA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 21769820 (0.000)110 (1.500) 44/88 NT 100% coding (71/321 nt) gatA galactitol‑specific enzyme IIA component of PTS
?W3110S.gb 2178182 = 0 (0.000)coding (202/315 nt) gatA galactitol‑specific enzyme IIA component of PTS

Only 100 of 162 total aligned reads displayed.

CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176908‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  W3110S.gb/2178182‑2178250
                                                                                                                                                
CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGAC                                                                           >  1:5734559/1‑71
 GTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                          <  1:1117675/71‑1
 GTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                          <  1:5156007/71‑1
 GTCGTAATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                          <  1:3614032/71‑1
       ATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCA                                                                     >  1:391766/1‑70
        TCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    <  1:7337380/70‑1
        TCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    <  1:4654524/70‑1
          ATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAA                                                                 <  1:1943542/71‑1
             ATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCG                                                              <  1:6625040/71‑1
              TCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCG                                                              <  1:2157830/70‑1
                CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                           <  1:6576084/71‑1
                CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                           <  1:3313271/71‑1
                CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                           <  1:1421842/71‑1
                    TGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        >  1:403090/1‑70
                      CTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAAT                                                     >  1:13595/1‑71
                      CTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                      <  1:3339871/70‑1
                        GAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                   >  1:5410735/1‑71
                        GAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAAAGC                                                   >  1:5446061/1‑71
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                  >  1:5893020/1‑71
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                  >  1:252050/1‑71
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                    <  1:858912/69‑1
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:5001407/1‑53
                           AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 >  1:2848509/1‑70
                            ATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                               >  1:2052345/1‑71
                            ATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGCTA                                               <  1:3414923/71‑1
                             TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                               <  1:4898125/70‑1
                              GAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATT                                             >  1:2460521/1‑71
                              GAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATT                                             >  1:3954937/1‑71
                               AACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                    <  1:5928830/63‑1
                               AACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                  <  1:5389708/49‑1
                                ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                           <  1:7748883/71‑1
                                 CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                           <  1:2204072/70‑1
                                   TTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:177426/1‑43
                                    TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         <  1:6956012/69‑1
                                    TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         <  1:3679343/69‑1
                                    TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTAT                                              >  1:2611980/1‑64
                                    TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                   >  1:7488123/1‑59
                                     ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    <  1:5278869/41‑1
                                     ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    <  1:3166059/41‑1
                                      TTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    <  1:7318446/40‑1
                                       TTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                               >  1:2311910/1‑60
                                        TGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                  >  1:5526570/1‑40
                                        TGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                  >  1:6030691/1‑40
                                          TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTG                                            >  1:7820050/1‑60
                                           TGGCCTTAACAGATAAATGGCTGCCGACTTAGCATGAATCGCCTCACAATGCGG                                                 >  1:2263558/1‑54
                                               CTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                   >  1:6834967/1‑48
                                                 TAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCA                           >  1:2753002/1‑70
                                                 TAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                            >  1:5485277/1‑69
                                                 TAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                       <  1:7026588/58‑1
                                                 TAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGAATAGCATGC                                 >  1:3591968/1‑63
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         >  1:7267822/1‑71
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         >  1:2031306/1‑71
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         >  1:6439099/1‑71
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                           >  1:3795437/1‑37
                                                   ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                           >  1:776949/1‑52
                                                   ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 >  1:3504850/1‑46
                                                      aATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                               <  1:2641688/44‑1
                                                      GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATT                     <  1:4476768/71‑1
                                                       ATAAATGGCTGACGACTTAGCATGAATCGCCTCAAAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTA                    >  1:6893087/1‑71
                                                        TAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTAT                   <  1:516053/71‑1
                                                        TAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTAT                   <  1:4131765/71‑1
                                                          AATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCC                 <  1:3195869/71‑1
                                                           ATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAAT                                        <  1:4051789/47‑1
                                                            TGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCG               <  1:3304935/71‑1
                                                             GGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG              >  1:1254708/1‑71
                                                             GGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:4555661/1‑44
                                                             GGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:5695615/1‑44
                                                             GGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:3526367/1‑44
                                                              GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGT             <  1:5235045/71‑1
                                                              GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGT             <  1:7108582/71‑1
                                                              GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGT             <  1:6771465/71‑1
                                                              GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG              >  1:6773869/1‑70
                                                              GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:2441162/1‑43
                                                               CTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATACCGTGCTGCTCAAGCATTATCCCGGTA            <  1:6149086/71‑1
                                                                TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                      <  1:3463718/60‑1
                                                                TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                      <  1:1933844/60‑1
                                                                TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                      <  1:7905514/44‑1
                                                                TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                      <  1:926686/44‑1
                                                                TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                      <  1:1379614/44‑1
                                                                TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                      <  1:2139515/44‑1
                                                                 GACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAAT                                        >  1:7328422/1‑41
                                                                 GACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAAT                                        >  1:3411823/1‑41
                                                                 GACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAAT                                        >  1:7515678/1‑41
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGG         <  1:5623060/71‑1
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGG          >  1:6554437/1‑70
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCG                                    >  1:3996213/1‑44
                                                                    GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCC                <  1:6427079/62‑1
                                                                       TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGT                                   <  1:2925293/40‑1
                                                                       TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGT                                   <  1:3922387/40‑1
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:1955313/1‑71
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:4300252/1‑71
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:3563295/1‑71
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCT   <  1:4935681/70‑1
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCC                 <  1:3690672/56‑1
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         >  1:4074018/1‑48
                                                                          GCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  <  1:536923/70‑1
                                                                          GCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  <  1:6157666/70‑1
                                                                          GCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTA            <  1:2366144/60‑1
                                                                          GCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTA            <  1:1534462/60‑1
                                                                          GCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTA            <  1:6479364/60‑1
                                                                                                                                                
CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176908‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  W3110S.gb/2178182‑2178250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.