New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 4121956 =134 (1.650)4 (0.050) 4/92 NT 3.4% coding (25/1089 nt) aroB 3‑dehydroquinate synthase
?W3110S.gb 4121973 = 104 (1.360)coding (42/1089 nt) aroB 3‑dehydroquinate synthase
Rejected: Frequency below/above cutoff threshold.

ATTTCAGCGGTAAGAATGAAGCTGGTTCATTAAACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4122028‑4121956
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cccAATTACCATCGCATCTGGTTTGTTTAATGAACCAGCTTCATTCTTACCGCTGAAATCGGGCGAGCAGG  >  W3110S.gb/4121973‑4122040
                                                                                                                                             
ATTTCAGCGGTAAGAATGAAGCTGGTTCATTAAACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCC                                                                        <  1:5934987/71‑1
 ttcCAGCGGTAAGAATGAAGCTGGTTCATTAAACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCC                                                                       >  1:1200355/4‑71
  TTCAGCGGTAAGAATGAAGCTGGTTCATTAAACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      >  1:4231682/1‑71
   TCAGCGGTAAGAATGAAGCTGGTTCATTAAACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      <  1:6811964/70‑1
   TCAGCGGTAAGAATGAAGCTGGTTCATTAAACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      >  1:4158842/1‑70
   TCAGCGGTAAGAATGAAGCTGGTTCATTAAACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      <  1:4871556/70‑1
     AGCGGTAAGAATGAAGCTGGTTCATTAAACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      >  1:4800441/1‑68
                      TGGTTCATTAAACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCC                                                                       <  1:3543415/50‑1
                                AACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      <  1:1618910/41‑1
                                AACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      <  1:2944479/41‑1
                                AACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      <  1:3825322/41‑1
                                AACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      <  1:4973407/41‑1
                                 ACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      >  1:4712047/1‑40
                                  CAAACCAGATGCGATGGTAATTGGGTAACTACGTTCC                                                                        >  1:5451996/1‑37
                                     ACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC                                                                      >  1:7498171/1‑36
                                     ACCAGATGCGATGGTAATTGGGTAACTACGTTCCC                                                                       >  1:198746/1‑35
                                                    AATTGGGTAACTACGTTCCCCAATTACCATCGCATCTGGTTTGTTTAATGAACCAGCTTCATTCT                          <  1:1316450/65‑1
                                                     ATTGGGTAACTACGTTCCCCAATTACCATCGCATCTGGTTTGT                                               >  1:2928078/1‑43
                                                      TTGGGTAACTACGTTCCCCAATTACCATCGCATCTGGTTTGTTTAA                                           <  1:786986/46‑1
                                                        GGGTAACTACGTTCCCAATTACCATCGCATCTGGTTTGTTTAATGAACCA                                    >  1:7750920/1‑50
                                                                       CCAATTACCATCGCATCTGGTTTGTTTAATGAACCAGCTTCATTCTTACCGCTGAAATCGGGCGAGCAGG  <  1:4663421/70‑1
                                                                                                                                             
ATTTCAGCGGTAAGAATGAAGCTGGTTCATTAAACAAACCAGATGCGATGGTAATTGGGTAACTACGTTCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4122028‑4121956
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cccAATTACCATCGCATCTGGTTTGTTTAATGAACCAGCTTCATTCTTACCGCTGAAATCGGGCGAGCAGG  >  W3110S.gb/4121973‑4122040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.