New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 4315117 =157 (1.930)4 (0.060) 3/84 NT 3.2% coding (544/1533 nt) alsA fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily
?W3110S.gb 4315147 = 106 (1.520)coding (514/1533 nt) alsA fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily
Rejected: Frequency below/above cutoff threshold.

CCAAAACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4315191‑4315117
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gaggtggGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCT  >  W3110S.gb/4315147‑4315216
                                                                                                                                                 
CCAAAACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAG                                                                            <  1:7572352/71‑1
 CAAAACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGG                                                                           >  1:5038500/1‑71
 CAAAACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAG                                                                              >  1:878062/1‑68
  AAAACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGT                                                                          >  1:7094690/1‑71
  AAAACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGT                                                                          >  1:4055686/1‑71
   AAACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGTG                                                                         >  1:6217595/1‑71
   AAACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGTG                                                                         >  1:3089857/1‑71
   AAACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGTG                                                                         <  1:4834406/71‑1
    AACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGTGG                                                                        <  1:3738470/71‑1
     ACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGTG                                                                         >  1:462140/1‑69
                 GATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGTG                                                                         >  1:3561968/1‑57
                             ATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGTG                                                                         <  1:1125158/45‑1
                                       ATGAACCCACCTCCTCACTCACCAATAAAGAGGTG                                                                         >  1:4718870/1‑35
                                              CACCTCCTCACTCACCAATAAAGAGGTGGGTTCATCCATGATGATGACTTTGGCAT                                             >  1:7197933/1‑56
                                              CACCTCCTCACTCACAATAAAGAGGTGGGTTCATCCATGATGATGACTTTGGCATC                                            >  1:2143382/1‑56
                                                 CTCCTCACTCACCAATAAAGAGGTGGGTTCTTCCATGATGATGACTTT                                                  <  1:2647755/48‑1
                                                   CCTCACTCACCAATAAAGAGGTGGGTTCATCCATGATGATGACTTTGG                                                >  1:237184/1‑48
                                                                    GAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTT                              >  1:113297/1‑49
                                                                      GGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGT      >  1:4486783/1‑71
                                                                        TGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTT                              <  1:5443086/45‑1
                                                                         GGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGC   >  1:4772622/1‑71
                                                                         GGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGC   >  1:3236261/1‑71
                                                                         GGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGC   >  1:7809396/1‑71
                                                                         GGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTA                  >  1:6533003/1‑56
                                                                          GGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCT  <  1:3535213/71‑1
                                                                                                                                                 
CCAAAACGCTGATGCTCGATGCCAAAGTCATCATCATGGATGAACCCACCTCCTCACTCACCAATAAAGAGGTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4315191‑4315117
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gaggtggGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCT  >  W3110S.gb/4315147‑4315216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.