New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 4425133 =123 (1.510)5 (0.070) 4/90 NT 4.6% coding (472/1398 nt) ulaA L‑ascorbate‑specific enzyme IIC component of PTS
?W3110S.gb 4425159 = 96 (1.280)coding (498/1398 nt) ulaA L‑ascorbate‑specific enzyme IIC component of PTS
Rejected: Frequency below/above cutoff threshold.

TGAGTCGGCTTGTACATCATGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAATGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4425206‑4425133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggtTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGT  >  W3110S.gb/4425159‑4425228
                                                                                                                                                
TGAGTCGGCTTGTACATCATGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAAT                                                                           >  1:1621440/1‑71
TGAGTCGGCTTGTACATCATGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAAT                                                                           >  1:1630640/1‑71
TGAGTCGGCTTGTACATCATGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAAT                                                                           >  1:1923486/1‑71
 GAGTCGGCTTGTACATCATGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAATG                                                                          >  1:6670024/1‑71
  AGTCGGCTTGTACATCATGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAATG                                                                          <  1:7485224/70‑1
       GCTTGTACATCATGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAATG                                                                          >  1:3481854/1‑65
           GTACATCATGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAATG                                                                          >  1:3022354/1‑61
           GTACATCATGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAATG                                                                          >  1:7299033/1‑61
                   TGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAATGGT                                                                        >  1:4488821/1‑55
                   TGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAATGGT                                                                        >  1:4798821/1‑55
                           GTGATGCCCCAGTAGAGCGAAACCAGAATCGGGTACAGATAATGG                                                                         >  1:5953916/1‑45
                                               AACCAGAATCGCGGTACAGATAATGGTTTCGCTCTACTGGGGCAT                                                      <  1:1861073/45‑1
                                               AACCAGAATCGCGGTACAGATAATGGTTTCGCTCTACTGGGGCAT                                                      <  1:1851816/45‑1
                                                ACCAGAATCGCGGTACAGATAATGGTTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGAC                           >  1:2791461/1‑71
                                                 CCAGAATCGCGGTACAGATAATGGTTTCGCTCTACTGGGGCATCA                                                    >  1:6993836/1‑45
                                                    GAATCGCGGTACAGATAATGGTTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAG                       <  1:3412663/71‑1
                                                                      TGGTTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGA                            >  1:5736895/1‑48
                                                                       GGTTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTG    >  1:6190145/1‑71
                                                                       GGTTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTG    >  1:6568640/1‑71
                                                                         TTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGT  <  1:1409790/71‑1
                                                                         TTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGT  <  1:6623616/71‑1
                                                                         TTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGT  <  1:5021512/71‑1
                                                                         TTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGT  <  1:4185691/71‑1
                                                                         TTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGT  >  1:4163043/1‑71
                                                                         TTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACG              <  1:543300/59‑1
                                                                         TTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAA                    <  1:4901255/53‑1
                                                                                                                                                
TGAGTCGGCTTGTACATCATGTTGGAAGTGATGCCCCAGTAGAGCGAAACCAGAATCGCGGTACAGATAATGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4425206‑4425133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggtTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGT  >  W3110S.gb/4425159‑4425228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.