New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 4431590133 (1.630)7 (0.090) 4/90 NT 4.8% coding (515/795 nt) yjfZ hypothetical protein
?W3110S.gb = 4431610 158 (2.110)coding (495/795 nt) yjfZ hypothetical protein
Rejected: Frequency below/above cutoff threshold.

TGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4431517‑4431590
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑attgCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAGGCGTTTCTAA  <  W3110S.gb/4431610‑4431542
                                                                                                                                               
TGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGA                                                                          >  1:2084049/1‑71
  CAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATT                                                                        <  1:5698567/71‑1
   AGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTG                                                                       <  1:1449822/71‑1
   AGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTG                                                                       <  1:4790373/71‑1
   AGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTG                                                                       <  1:581602/71‑1
                     GGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTG                                                                       <  1:120487/53‑1
                     GGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTG                                                                       <  1:2328964/53‑1
                         TTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGCTGGTCATCTGAACTACCAACA                                                 <  1:2321627/71‑1
                         TTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGAT                                                                         <  1:7508495/47‑1
                           AGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGCTGGTCATCTGAACTACCAACAAT                                               >  1:6103734/1‑71
                                               GAAGTTGGTCGATATAAAGATCGATTGCTGGTCATCTGAACTACCAACA                                                 <  1:6156572/49‑1
                                               GAAGTTGGTCGATATAAAGATCGATTGCTGGTCATCTGAACTACCAAC                                                  <  1:3136514/48‑1
                                                 AGTTGGTCGATATAAAGATCGATTGCTGGTCATCTGAACTACCAACAA                                                >  1:3482837/1‑48
                                                 AGTTGGTCGATATAAAGATCGATTGCTGGTCATCTGAACTACCAACAA                                                >  1:4552982/1‑48
                                                 AGTTGGTCGATATAAAGATCGATTGCTGGTCATCTGAACTACCAACAA                                                >  1:4226385/1‑48
                                                                      ATTGCTGGTCCTCTGAACTACCAACAATCGATCTTTATATCGA                                >  1:236022/1‑43
                                                                      ATTGCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAGGCGTTTCT    <  1:1372288/71‑1
                                                                      ATTGCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACT                          <  1:435887/49‑1
                                                                      ATTGCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGAC                               >  1:7345519/1‑44
                                                                      ATTGCTGGTCATCTGAACTACCAACAATCGATCTTTATATCG                                 >  1:2469263/1‑42
                                                                       TTGCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAGGCGTTTCT    >  1:7276826/1‑70
                                                                       TTGCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAG            <  1:4716386/62‑1
                                                                       TTGCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAG            <  1:3034927/62‑1
                                                                       TTGCTGGTCATCTGAACTACCAACAATCGATCTTT                                       <  1:4597879/35‑1
                                                                       TTGCTGGTCATCTGAACTACCAACAATCGATCTTT                                       <  1:5590914/35‑1
                                                                       TTGCTGGTCATCTGAACTACCAACAATCGATCTTT                                       <  1:3029776/35‑1
                                                                       TTGCTGGTCATCTGAACTACCAACAATCGATCTTT                                       <  1:2032040/35‑1
                                                                       TTGCTGGTCATCTGAACTACCAACAATCGATCTTT                                       <  1:820022/35‑1
                                                                        TGCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAGGC          >  1:4139583/1‑63
                                                                        TGCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAGGC          >  1:3362145/1‑63
                                                                         GCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAGGCGTTTCTAA  <  1:461191/70‑1
                                                                         GCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAGGCGTTTCTAA  <  1:4034731/70‑1
                                                                         GCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAGGCGTTTCTAA  <  1:7567446/70‑1
                                                                                                                                               
TGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4431517‑4431590
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑attgCTGGTCATCTGAACTACCAACAATCGATCTTTATATCGACCAACTTCGCACTGTTACAGGCGTTTCTAA  <  W3110S.gb/4431610‑4431542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.