New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	W3110S.gb	4463110 =	99 (1.220)	4 (0.050)	3/96	NT	3.6%	coding (470/1353 nt)	pmbA	predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17
	?	W3110S.gb	4463147 =	117 (1.470)					coding (507/1353 nt)	pmbA	predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17
Rejected: Frequency below/above cutoff threshold.

TGACACCGTAGTGGCTGTTAAAGCTGCCACCTTCGGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4463148‑4463110 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGT  >  W3110S.gb/4463147‑4463216                                                                                                               TGACACCGTAGTGGCTGTTAAAGCTGCCACCTTCGGTATCAAAGTTTTTGGCAACAGCCACGGCATGTTG                                         >  1:1758559/1‑70         TAGTGGCTGTTAAAGCTGCCACCTTCGGTATCAAAGTTTTTGGC                                                           <  1:4096586/44‑1          AGTGGCTGTTAAAGCTGCCACCTTCGGTATCAAAGTTTTTGGCAA                                                         >  1:1906706/1‑45          AGTGGCTGTTAAAGCTGCCACCTTCGGTATCAAAGTTTTTGGCAA                                                         >  1:910260/1‑45                                       TCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGT  >  1:1089655/1‑71                                       TCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGT  >  1:74149/1‑71                                                                                                               TGACACCGTAGTGGCTGTTAAAGCTGCCACCTTCGGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4463148‑4463110 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGT  >  W3110S.gb/4463147‑4463216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.