New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 4590442117 (1.440)5 (0.070) 4/88 NT 4.3% coding (1056/3567 nt) hsdR endonuclease R
?W3110S.gb = 4590457 115 (1.570)coding (1041/3567 nt) hsdR endonuclease R
Rejected: Frequency below/above cutoff threshold.

CTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4590370‑4590442
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tatcgCTACAGTAAATGTTTCGATAATGGCTTCCTGCGGGAAACCTTGCTTGAGCACTACTCACCGGATGAAGTG  <  W3110S.gb/4590457‑4590388
                                                                                                                                               
CTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTAT                                                                          >  1:54467/1‑71
CTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTAT                                                                          >  1:4010097/1‑71
CTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTA                                                                           <  1:6434199/70‑1
  CTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATC                                                                         >  1:277827/1‑70
                  CACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCG                                                                        <  1:4410878/55‑1
                       CATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGCTACAGTAAATGTTTCGATA                                                    <  1:763249/70‑1
                        ATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGCTACAGTAAATGTTTCGATAA                                                   <  1:1258644/70‑1
                        ATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGCTACAGTAAATGTTTCGATAA                                                   <  1:2124185/70‑1
                           CGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGCTACAGTAAATGTTTCGATAAT                                                  >  1:1285324/1‑68
                                        TCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGCTACAGTAAATGT                                                           <  1:3825518/46‑1
                                                                     ATCGCTACAGTAAATGTTTCGATAATGGCTTCCTGCGGGAAAACTTGCTTGAGCACTACTCACCGGATG       >  1:5841177/1‑69
                                                                     ATCGCTACAGTAAATGTTTCGATAATGGCTTCCTGCGGGAAA                                  >  1:4989350/1‑42
                                                                      TCGCTACAGTAAATGTTTCGATAATGGCTTCCTGCGGGAAACCTTGCTTGAGCACTACTCACCGGATGAAG    >  1:4944950/1‑71
                                                                      TCGCTACAGTAAATGTTTCGATAATGGCTTCCTGCGGGAAACCTTGCTTGAGCACTACTCACC            <  1:3259614/63‑1
                                                                      TCGCTACAGTAAATGTTTCGATAATGGCTTCCTGCGGGAAACCTTGCTTGAGCAC                    <  1:4977145/55‑1
                                                                       CGCTACAGTAAATGTTTCGATAATGGCTTCCTGCGGGAAACCTTGCTTGAGCACTACTCACCGGATGAAGT   >  1:2068655/1‑71
                                                                        GCTACAGTAAATGTTTCGATAATGGCTTCCTGCGGGAAACCTTGCTTGAGCACTACTCACCGGATGAAGTG  <  1:7829127/71‑1
                                                                                                                                               
CTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4590370‑4590442
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tatcgCTACAGTAAATGTTTCGATAATGGCTTCCTGCGGGAAACCTTGCTTGAGCACTACTCACCGGATGAAGTG  <  W3110S.gb/4590457‑4590388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.