Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 70,434 C→T 79.4% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb70,4340CT79.4% 844.9 / 217.2 394N16N (AAC→AATaraCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base C (33/48);  new base T (161/152);  total (194/200)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 395 total aligned reads displayed.

AATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGC  >  W3110S.gb/70366‑70504
                                                                    |                                                                      
aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                      >  1:1130653/1‑71 (MQ=255)
aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                      >  1:7524674/1‑71 (MQ=255)
aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                      >  1:5927750/1‑71 (MQ=255)
aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                      >  1:2084864/1‑71 (MQ=255)
aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACg                                                                       <  1:6112535/70‑1 (MQ=255)
  tggtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGTGATACTCGTTTAATGccc                                                                    <  1:372795/71‑1 (MQ=255)
    gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCAt                                                                  <  1:7198774/71‑1 (MQ=255)
    gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCAt                                                                  >  1:3834159/1‑71 (MQ=255)
    gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCAt                                                                  <  1:7601172/71‑1 (MQ=255)
    gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCAt                                                                  <  1:6912927/71‑1 (MQ=255)
       ggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtg                                                               <  1:2322934/71‑1 (MQ=255)
        gAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtgg                                                              <  1:2876954/71‑1 (MQ=255)
           tATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggtgg                                                           <  1:668885/71‑1 (MQ=255)
            aTGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGcc                                                                     <  1:4763134/60‑1 (MQ=255)
             tGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGc                                                          <  1:5390941/70‑1 (MQ=255)
             tGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCg                                                         <  1:4709689/71‑1 (MQ=255)
               aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                       >  1:1060459/1‑71 (MQ=255)
               aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGACACTCGTTTAATGCCCATCTGGTGGCggg                                                       >  1:3101986/1‑71 (MQ=255)
                aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                       <  1:4670963/70‑1 (MQ=255)
                aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGt                                                      <  1:1869505/71‑1 (MQ=255)
                 aaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGtt                                                     <  1:1979390/71‑1 (MQ=255)
                  aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                    <  1:906414/71‑1 (MQ=255)
                  aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                    <  1:5971561/71‑1 (MQ=255)
                   gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTa                                                   <  1:2571474/71‑1 (MQ=255)
                   gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTa                                                   <  1:3877064/71‑1 (MQ=255)
                      tGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACg                                                >  1:681101/1‑71 (MQ=255)
                      tGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACg                                                >  1:795751/1‑71 (MQ=255)
                       ggCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACg                                                >  1:6417974/1‑70 (MQ=255)
                          tGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCtgg                                                       <  1:1720473/60‑1 (MQ=255)
                            acgcgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          <  1:2469155/69‑1 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                      >  1:6174663/1‑43 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCg                                                         <  1:3169445/56‑1 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          >  1:103328/1‑71 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          >  1:1200592/1‑71 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACg                                                >  1:7789830/1‑65 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACg                                                >  1:768776/1‑65 (MQ=255)
                             aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtg                                                               >  1:6271393/1‑49 (MQ=255)
                             aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtg                                                               >  1:828981/1‑49 (MQ=255)
                             aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         <  1:4135919/71‑1 (MQ=255)
                             aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         <  1:7367197/71‑1 (MQ=255)
                              gcgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          >  1:7023004/1‑69 (MQ=255)
                              gcgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                        <  1:3962568/71‑1 (MQ=255)
                               cgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                      <  1:2841961/40‑1 (MQ=255)
                                 cAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         <  1:5878475/67‑1 (MQ=255)
                                 cAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGc                                     >  1:169432/1‑71 (MQ=255)
                                 cAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGc                                     >  1:4426384/1‑71 (MQ=255)
                                  aaaaTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                    <  1:4506870/55‑1 (MQ=255)
                                    aaTGATCCCCTGCTGCCGTGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCa                                   >  1:6757778/1‑70 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCa                                                                   >  1:1433280/1‑38 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtgg                                                              >  1:4375626/1‑43 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                                  >  1:7546750/1‑71 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                                  <  1:1814103/71‑1 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                                  >  1:4283136/1‑71 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                                  >  1:6036665/1‑71 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                                  <  1:3337576/71‑1 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                                  >  1:7124295/1‑71 (MQ=255)
                                     aTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAc                                 >  1:571533/1‑71 (MQ=255)
                                     aTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAc                                 <  1:6833296/71‑1 (MQ=255)
                                      tGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCg                                                         >  1:4604826/1‑46 (MQ=255)
                                        aTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                        >  1:1507022/1‑61 (MQ=255)
                                        aTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACgg                               <  1:4879348/70‑1 (MQ=255)
                                         tCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                                  <  1:5093194/66‑1 (MQ=255)
                                            ccTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGc                                               >  1:1657772/1‑50 (MQ=255)
                                            ccTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGc                                               >  1:3037196/1‑50 (MQ=255)
                                            ccTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGt                                                      <  1:2672337/43‑1 (MQ=255)
                                             ctgctgCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtct                         >  1:405310/1‑71 (MQ=255)
                                               gctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGa                                            >  1:6533443/1‑50 (MQ=255)
                                               gctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtct                         >  1:6985356/1‑69 (MQ=255)
                                                ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                             >  1:7159108/1‑64 (MQ=255)
                                                ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTa                            >  1:1448393/1‑65 (MQ=255)
                                                 tgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGtt                                                     <  1:3441516/39‑1 (MQ=255)
                                                   ccGGGATACTCTTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACg                                <  1:7617845/58‑1 (MQ=255)
                                                   ccGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAt                     <  1:1092229/69‑1 (MQ=255)
                                                      ggATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttttt                <  1:1364610/71‑1 (MQ=255)
                                                       gATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt               <  1:4799149/71‑1 (MQ=255)
                                                        aTACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttt                  >  1:2193376/1‑67 (MQ=255)
                                                        aTACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACgg                               <  1:499666/54‑1 (MQ=255)
                                                         tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt               <  1:2375786/69‑1 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGc                                               >  1:490328/1‑35 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcg           <  1:2770793/71‑1 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc            >  1:4842944/1‑70 (MQ=255)
                                                           cTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAt                           <  1:5605906/55‑1 (MQ=255)
                                                            tCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGa                                            >  1:5310762/1‑37 (MQ=255)
                                                            tCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGa                                            >  1:2052116/1‑37 (MQ=255)
                                                            tCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa              <  1:5512327/67‑1 (MQ=255)
                                                            tCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa              <  1:6722497/67‑1 (MQ=255)
                                                            tCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcga          >  1:1228789/1‑71 (MQ=255)
                                                             cGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                        >  1:5243555/1‑40 (MQ=255)
                                                              gTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttt                 >  1:2547991/1‑62 (MQ=255)
                                                              gTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgacc        >  1:4566512/1‑71 (MQ=255)
                                                               tttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGt                              <  1:4545674/48‑1 (MQ=255)
                                                               tttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccg       <  1:6853654/71‑1 (MQ=255)
                                                               tttAACGCCCATCTGGTGGCGTGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttt                  <  1:2975737/60‑1 (MQ=255)
                                                                ttAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttt                  <  1:2257405/59‑1 (MQ=255)
                                                                 tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTa                            >  1:1937129/1‑48 (MQ=255)
                                                                 tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTa                            >  1:3600137/1‑48 (MQ=255)
                                                                 tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt               >  1:6288225/1‑61 (MQ=255)
                                                                 tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac     >  1:3479461/1‑71 (MQ=255)
                                                                   aCGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtct                         >  1:3287738/1‑49 (MQ=255)
                                                                   aCGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtct                         >  1:850398/1‑49 (MQ=255)
                                                                    |                                                                      
AATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGC  >  W3110S.gb/70366‑70504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: