Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,915,568 A→T 8.9% P321P (CCT→CCA prc ← carboxy‑terminal protease for penicillin‑binding protein 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,915,5680AT 8.9% 214.9 / 11.9 79P321P (CCT→CCAprccarboxy‑terminal protease for penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base A (45/27);  new base T (3/4);  total (48/31)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

CCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACG  >  W3110S.gb/1915498‑1915636
                                                                      |                                                                    
ccGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACcagca                                                                      <  1:4759905/71‑1 (MQ=255)
  gcgcgGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAgg                                                                    >  1:4581313/1‑71 (MQ=255)
   cgcgGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGt                                                                   <  1:6794073/71‑1 (MQ=255)
      ggTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTaaa                                                                <  1:5626757/71‑1 (MQ=255)
          ttCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTaaaa                                                               >  1:6008694/1‑68 (MQ=255)
          ttCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAAtt                                                             <  1:1364996/70‑1 (MQ=255)
           tCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTc                                                           >  1:7278703/1‑71 (MQ=255)
           tCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTc                                                           >  1:5856756/1‑71 (MQ=255)
            cGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAAttt                                                            <  1:4433836/69‑1 (MQ=255)
            cGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAAttt                                                            <  1:5684671/69‑1 (MQ=255)
            cGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAAtt                                                             <  1:1870301/68‑1 (MQ=255)
            cGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTc                                                           <  1:3544833/70‑1 (MQ=255)
            cGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTc                                                           >  1:2802344/1‑70 (MQ=255)
            cGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTc                                                           >  1:1344837/1‑70 (MQ=255)
            cGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTc                                                           <  1:7786055/70‑1 (MQ=255)
            agagaCGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTc                                                           <  1:3850739/69‑1 (MQ=255)
             gagaCGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTc                                                           >  1:103381/1‑69 (MQ=255)
               gaCGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGa                                                       >  1:3640766/1‑71 (MQ=255)
                aCGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGAc                                                      >  1:2902382/1‑71 (MQ=255)
                   aaTACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGAc                                                      <  1:3701433/68‑1 (MQ=255)
                    aTACGTTCACGGGTCAACGTTACAGTACGGTTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAAc                                                  >  1:3905848/1‑71 (MQ=255)
                    aTACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAAc                                                  >  1:6906668/1‑71 (MQ=255)
                    aTACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAAc                                                  >  1:469840/1‑71 (MQ=255)
                    aTACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAAc                                                  >  1:4629640/1‑71 (MQ=255)
                     tACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTc                                                           >  1:6849067/1‑61 (MQ=255)
                     tACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGa                                                    >  1:5592574/1‑68 (MQ=255)
                      aCGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTcc                                                          >  1:6471452/1‑61 (MQ=255)
                       cGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGa                                                       >  1:6243500/1‑63 (MQ=255)
                        gTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACtt                                                <  1:822352/69‑1 (MQ=255)
                           cACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTa                                              >  1:3177351/1‑68 (MQ=255)
                              gggTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCTGCTGGTaaa                                                                <  1:1010443/47‑1 (MQ=38)
                              gggTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCTGCTGGTaaa                                                                <  1:7683082/47‑1 (MQ=38)
                              gggTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCTGCTGGTaaa                                                                <  1:5802499/47‑1 (MQ=38)
                              gggTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGccc                                        <  1:861821/71‑1 (MQ=255)
                               ggTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCTGCTGGTaaagg                                                              >  1:1434925/1‑46 (MQ=37)
                               ggTCAACGTTACAGTACGGGTCTTGGACCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCt                                       >  1:5434987/1‑71 (MQ=255)
                                gTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCa                                                         <  1:4083313/52‑1 (MQ=255)
                                 tCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAAtt                                                             <  1:4453830/47‑1 (MQ=255)
                                 tCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAAtt                                                             <  1:5534709/47‑1 (MQ=255)
                                   aaCGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAAttt                                                            <  1:5551158/46‑1 (MQ=255)
                                    aCGTTACAGTACGGGTCTTGGTCCCTTTACCTGCTGGTaaa                                                                <  1:3222496/41‑1 (MQ=38)
                                     cGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAAttt                                                            >  1:3838273/1‑44 (MQ=255)
                                     cGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTa                                              >  1:1483035/1‑58 (MQ=255)
                                     cGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTa                                              >  1:2187122/1‑58 (MQ=255)
                                       ttACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACTAACTTTACTGCCCTTCGGCCCt                               >  1:6295622/1‑71 (MQ=255)
                                       ttACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCt                               <  1:6256827/71‑1 (MQ=255)
                                       ttACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCt                               <  1:7692392/71‑1 (MQ=255)
                                         aCAGTACGGGTCTTGGTCCCTTTTCCAGCAGGTAAAATTTCCAGACGAACt                                                 >  1:1651740/1‑51 (MQ=255)
                                         aCAGTACGGGTCTTGGTCCCTTTACCTGCTGGTaaag                                                               >  1:4305381/1‑36 (MQ=25)
                                         aCAGTACGGGTCTTGGTCCCTTTACCTGCTGGTaaag                                                               >  1:7894096/1‑36 (MQ=25)
                                         aCAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAAc                                                  >  1:7247021/1‑50 (MQ=255)
                                         aCAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACt                                                 >  1:6528861/1‑51 (MQ=255)
                                         aCAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACt                                                 >  1:2043267/1‑51 (MQ=255)
                                         aCAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACt                                                 >  1:7895572/1‑51 (MQ=255)
                                          cAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGaa                                                   >  1:3802421/1‑48 (MQ=255)
                                               cGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCa                                                         >  1:3011906/1‑37 (MQ=255)
                                               cGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCa                                                         >  1:7284497/1‑37 (MQ=255)
                                               cGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaatta                        <  1:4678095/70‑1 (MQ=255)
                                               cGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaatta                        <  1:7439589/70‑1 (MQ=255)
                                                gggTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaatt                         <  1:2423524/68‑1 (MQ=255)
                                                  gTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAgg                     >  1:2936139/1‑70 (MQ=255)
                                                  gTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAgg                     >  1:5403676/1‑70 (MQ=255)
                                                   tCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaatt                         <  1:5209566/65‑1 (MQ=255)
                                                   tCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGc                    >  1:5493691/1‑70 (MQ=255)
                                                   tCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCa                   <  1:5239173/71‑1 (MQ=255)
                                                    cTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaattaa                       >  1:4676377/1‑66 (MQ=255)
                                                     ttGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCt                               >  1:329843/1‑57 (MQ=255)
                                                       ggTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaatt                         >  1:6014657/1‑61 (MQ=255)
                                                       ggTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAAc                 <  1:6852087/69‑1 (MQ=255)
                                                          cccTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACcaca             >  1:5887956/1‑70 (MQ=255)
                                                           ccTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCAcatc           <  1:134903/71‑1 (MQ=255)
                                                             tttACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGc                                  >  1:6298256/1‑46 (MQ=255)
                                                              ttACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCAcatcat         >  1:3243204/1‑70 (MQ=255)
                                                              ttACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCAcatcat         >  1:3664307/1‑70 (MQ=255)
                                                              ttACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCAcatcat         >  1:2680384/1‑70 (MQ=255)
                                                               tACCAGCAGGTAAAATTTCCAGACGAACTTTACTGcc                                         >  1:3681338/1‑37 (MQ=255)
                                                               tACCAGCAGGTAAAATTTCCAGACGAACTTTACTGcc                                         >  1:7268442/1‑37 (MQ=255)
                                                               tACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCAcatcat         <  1:4784655/69‑1 (MQ=255)
                                                               tACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACatcatc        >  1:7288374/1‑70 (MQ=255)
                                                                 ccAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCaa      <  1:2161745/70‑1 (MQ=255)
                                                                   agcagGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGAc   >  1:6296517/1‑71 (MQ=255)
                                                                    gcagGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACg  >  1:6232600/1‑71 (MQ=255)
                                                                    gcagGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACg  >  1:3423657/1‑71 (MQ=255)
                                                                      |                                                                    
CCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACG  >  W3110S.gb/1915498‑1915636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: