Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,988,855 | T→A | 10.6% | intergenic (‑590/‑207) | araF ← / → yecI | L‑arabinose transporter subunit/predicted ferritin‑like protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,988,855 | 0 | T | A | 10.6% | 172.1 / 10.5 | 65 | intergenic (‑590/‑207) | araF/yecI | L‑arabinose transporter subunit/predicted ferritin‑like protein |
Reads supporting (aligned to +/- strand): ref base T (28/30); new base A (4/3); total (32/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
AATTCATTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGTTA > W3110S.gb/1988786‑1988923 | aattcaTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCATTTACATTGAttt > 1:3915502/1‑70 (MQ=255) aattcaTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGAttt > 1:6748882/1‑70 (MQ=255) aattcaTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGAttt < 1:6423912/70‑1 (MQ=255) attcaTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtca < 1:4821808/71‑1 (MQ=255) ttcaTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcat < 1:2785145/71‑1 (MQ=255) aTTGATTAATATCAACAATATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcatca < 1:4147477/70‑1 (MQ=255) aTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCaa < 1:583636/71‑1 (MQ=255) tatCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTTCATTGATGAAATCAATgt < 1:2909417/65‑2 (MQ=37) tatCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTTCATTGATGAAATCAATgt < 1:3396385/65‑2 (MQ=37) tatCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTTCATTGATGAAATCAATgt < 1:3433587/65‑2 (MQ=37) tCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTaa > 1:7240681/1‑70 (MQ=255) acaaGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATg < 1:1686930/59‑1 (MQ=255) acaaGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATg < 1:1313080/59‑1 (MQ=255) caaGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcatc > 1:6644610/1‑54 (MQ=255) aaGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAatat < 1:2628983/71‑1 (MQ=255) gATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcat < 1:1078834/50‑1 (MQ=255) gATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCaa < 1:7366718/53‑1 (MQ=255) tACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtc < 1:1265744/46‑1 (MQ=255) aCAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcat > 1:3978604/1‑47 (MQ=255) aCAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATaaa > 1:927336/1‑71 (MQ=255) cAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcat > 1:549573/1‑46 (MQ=255) cAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcat > 1:2947485/1‑46 (MQ=255) aaaaaGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACt > 1:1796490/1‑71 (MQ=255) aaaaGCACTATCATTAAAATTCATTGCAGTTACATTGATTtc < 1:4844123/42‑1 (MQ=255) tATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTtgat < 1:5920947/66‑1 (MQ=255) tATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTtgat < 1:6055041/66‑1 (MQ=255) tATCATTAAAATTCAGTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAtt > 1:7333167/1‑70 (MQ=255) tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAatatat > 1:90617/1‑55 (MQ=255) tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt < 1:6177518/69‑1 (MQ=255) tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt < 1:5779494/69‑1 (MQ=255) tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt < 1:43423/69‑1 (MQ=255) tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt > 1:6355649/1‑69 (MQ=255) tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt > 1:1043037/1‑69 (MQ=255) ttAAAATTCATTGCAGTTACATTGATGAAATCAATGtaa > 1:2475063/1‑39 (MQ=17) ttAAAATTCATTGCAGTTACATTGATGAAATCAATGtaa > 1:5600409/1‑39 (MQ=17) ttAAAATTCATTGCAGTTACATTGATGAAATCAATGtaa > 1:2875776/1‑39 (MQ=17) tAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTaa > 1:574555/1‑67 (MQ=255) tAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTaa > 1:1873836/1‑67 (MQ=255) aaaaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAtt > 1:2626850/1‑71 (MQ=255) aaaaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAt < 1:6214544/70‑1 (MQ=255) aaaaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAt < 1:4739972/70‑1 (MQ=255) aaaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAttt < 1:6761248/71‑1 (MQ=255) aaaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAttt > 1:7135564/1‑71 (MQ=255) aaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAttt < 1:6751978/70‑1 (MQ=255) aTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTc < 1:6975636/71‑1 (MQ=255) ttCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAttt < 1:3435670/68‑1 (MQ=255) ttCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCt < 1:1712902/71‑1 (MQ=255) tCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCt > 1:3645358/1‑70 (MQ=255) cATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt > 1:5197090/1‑58 (MQ=255) cATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAg > 1:6867325/1‑61 (MQ=255) cATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAg > 1:4925055/1‑61 (MQ=255) cATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAg > 1:3089435/1‑61 (MQ=255) gttGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACtt < 1:5184520/48‑1 (MQ=255) aTTGCAGTTACATTGATTTCATCAATGAAATGTAAAAatatat < 1:4192547/43‑1 (MQ=255) aTTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTta < 1:6049523/70‑1 (MQ=255) gCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATAcc > 1:4642655/1‑71 (MQ=255) cAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACtt > 1:2160600/1‑45 (MQ=255) aGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCg > 1:7755164/1‑71 (MQ=255) gTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGt < 1:7180501/71‑1 (MQ=255) ttACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTta < 1:2984784/63‑1 (MQ=255) ttACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt < 1:79622/71‑1 (MQ=255) ttACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt < 1:1296585/71‑1 (MQ=255) ttcATTGATGAAATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt > 1:4796742/3‑70 (MQ=255) tACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt < 1:6101742/70‑1 (MQ=255) tACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt < 1:1697480/70‑1 (MQ=255) aCATTGATTTCATCAATGAAATGTAAAAATATATAAATTTGATGATTTAAGCATTTTCTTATACCCGtt < 1:2539198/69‑1 (MQ=255) aCATTGATTTCATCAATGAAATGTAAAAATATATAAACTtga > 1:4868374/1‑42 (MQ=255) aCATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTta > 1:6915065/1‑61 (MQ=255) aCATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt > 1:1927094/1‑69 (MQ=255) aTTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGa > 1:2205713/1‑71 (MQ=255) aTTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGa > 1:5187191/1‑71 (MQ=255) tGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAtttt > 1:5118266/1‑53 (MQ=255) tGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCt > 1:4740237/1‑55 (MQ=255) tGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACg > 1:7358207/1‑71 (MQ=255) aTTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAtttt < 1:6432335/51‑1 (MQ=255) aTTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGtt < 1:4728103/71‑1 (MQ=255) aTTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGt < 1:4727784/70‑1 (MQ=255) aTTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGt > 1:4390581/1‑70 (MQ=255) tttCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGTTa < 1:5074592/71‑1 (MQ=255) tcatcaATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCt < 1:7162021/50‑1 (MQ=255) | AATTCATTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGTTA > W3110S.gb/1988786‑1988923 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |