Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,988,855 T→A 10.6% intergenic (‑590/‑207) araF ← / → yecI L‑arabinose transporter subunit/predicted ferritin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,988,8550TA10.6% 172.1 / 10.5 65intergenic (‑590/‑207)araF/yecIL‑arabinose transporter subunit/predicted ferritin‑like protein
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base A (4/3);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AATTCATTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGTTA  >  W3110S.gb/1988786‑1988923
                                                                     |                                                                    
aattcaTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCATTTACATTGAttt                                                                      >  1:3915502/1‑70 (MQ=255)
aattcaTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGAttt                                                                      >  1:6748882/1‑70 (MQ=255)
aattcaTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGAttt                                                                      <  1:6423912/70‑1 (MQ=255)
 attcaTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtca                                                                    <  1:4821808/71‑1 (MQ=255)
  ttcaTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcat                                                                   <  1:2785145/71‑1 (MQ=255)
     aTTGATTAATATCAACAATATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcatca                                                                 <  1:4147477/70‑1 (MQ=255)
     aTTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCaa                                                                <  1:583636/71‑1 (MQ=255)
              tatCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTTCATTGATGAAATCAATgt                                                             <  1:2909417/65‑2 (MQ=37)
              tatCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTTCATTGATGAAATCAATgt                                                             <  1:3396385/65‑2 (MQ=37)
              tatCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTTCATTGATGAAATCAATgt                                                             <  1:3433587/65‑2 (MQ=37)
                tCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTaa                                                      >  1:7240681/1‑70 (MQ=255)
                   acaaGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATg                                                              <  1:1686930/59‑1 (MQ=255)
                   acaaGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATg                                                              <  1:1313080/59‑1 (MQ=255)
                    caaGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcatc                                                                  >  1:6644610/1‑54 (MQ=255)
                     aaGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAatat                                                <  1:2628983/71‑1 (MQ=255)
                       gATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcat                                                                   <  1:1078834/50‑1 (MQ=255)
                       gATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCaa                                                                <  1:7366718/53‑1 (MQ=255)
                         tACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtc                                                                     <  1:1265744/46‑1 (MQ=255)
                          aCAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcat                                                                   >  1:3978604/1‑47 (MQ=255)
                          aCAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATaaa                                           >  1:927336/1‑71 (MQ=255)
                           cAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcat                                                                   >  1:549573/1‑46 (MQ=255)
                           cAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTtcat                                                                   >  1:2947485/1‑46 (MQ=255)
                            aaaaaGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACt                                         >  1:1796490/1‑71 (MQ=255)
                             aaaaGCACTATCATTAAAATTCATTGCAGTTACATTGATTtc                                                                     <  1:4844123/42‑1 (MQ=255)
                                     tATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTtgat                                     <  1:5920947/66‑1 (MQ=255)
                                     tATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTtgat                                     <  1:6055041/66‑1 (MQ=255)
                                     tATCATTAAAATTCAGTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAtt                                 >  1:7333167/1‑70 (MQ=255)
                                       tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAatatat                                              >  1:90617/1‑55 (MQ=255)
                                       tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt                                <  1:6177518/69‑1 (MQ=255)
                                       tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt                                <  1:5779494/69‑1 (MQ=255)
                                       tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt                                <  1:43423/69‑1 (MQ=255)
                                       tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt                                >  1:6355649/1‑69 (MQ=255)
                                       tCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt                                >  1:1043037/1‑69 (MQ=255)
                                          ttAAAATTCATTGCAGTTACATTGATGAAATCAATGtaa                                                           >  1:2475063/1‑39 (MQ=17)
                                          ttAAAATTCATTGCAGTTACATTGATGAAATCAATGtaa                                                           >  1:5600409/1‑39 (MQ=17)
                                          ttAAAATTCATTGCAGTTACATTGATGAAATCAATGtaa                                                           >  1:2875776/1‑39 (MQ=17)
                                           tAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTaa                              >  1:574555/1‑67 (MQ=255)
                                           tAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTaa                              >  1:1873836/1‑67 (MQ=255)
                                            aaaaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAtt                         >  1:2626850/1‑71 (MQ=255)
                                            aaaaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAt                          <  1:6214544/70‑1 (MQ=255)
                                            aaaaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAt                          <  1:4739972/70‑1 (MQ=255)
                                             aaaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAttt                        <  1:6761248/71‑1 (MQ=255)
                                             aaaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAttt                        >  1:7135564/1‑71 (MQ=255)
                                              aaTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAttt                        <  1:6751978/70‑1 (MQ=255)
                                               aTTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTc                      <  1:6975636/71‑1 (MQ=255)
                                                ttCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAttt                        <  1:3435670/68‑1 (MQ=255)
                                                ttCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCt                     <  1:1712902/71‑1 (MQ=255)
                                                 tCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCt                     >  1:3645358/1‑70 (MQ=255)
                                                  cATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGAttt                                >  1:5197090/1‑58 (MQ=255)
                                                  cATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAg                             >  1:6867325/1‑61 (MQ=255)
                                                  cATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAg                             >  1:4925055/1‑61 (MQ=255)
                                                  cATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAg                             >  1:3089435/1‑61 (MQ=255)
                                                   gttGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACtt                                        <  1:5184520/48‑1 (MQ=255)
                                                   aTTGCAGTTACATTGATTTCATCAATGAAATGTAAAAatatat                                              <  1:4192547/43‑1 (MQ=255)
                                                   aTTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTta                   <  1:6049523/70‑1 (MQ=255)
                                                      gCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATAcc               >  1:4642655/1‑71 (MQ=255)
                                                       cAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACtt                                        >  1:2160600/1‑45 (MQ=255)
                                                        aGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCg             >  1:7755164/1‑71 (MQ=255)
                                                         gTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGt            <  1:7180501/71‑1 (MQ=255)
                                                          ttACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTta                   <  1:2984784/63‑1 (MQ=255)
                                                          ttACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt           <  1:79622/71‑1 (MQ=255)
                                                          ttACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt           <  1:1296585/71‑1 (MQ=255)
                                                           ttcATTGATGAAATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt           >  1:4796742/3‑70 (MQ=255)
                                                           tACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt           <  1:6101742/70‑1 (MQ=255)
                                                           tACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt           <  1:1697480/70‑1 (MQ=255)
                                                            aCATTGATTTCATCAATGAAATGTAAAAATATATAAATTTGATGATTTAAGCATTTTCTTATACCCGtt           <  1:2539198/69‑1 (MQ=255)
                                                            aCATTGATTTCATCAATGAAATGTAAAAATATATAAACTtga                                      >  1:4868374/1‑42 (MQ=255)
                                                            aCATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTta                   >  1:6915065/1‑61 (MQ=255)
                                                            aCATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGtt           >  1:1927094/1‑69 (MQ=255)
                                                              aTTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGa       >  1:2205713/1‑71 (MQ=255)
                                                              aTTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGa       >  1:5187191/1‑71 (MQ=255)
                                                                tGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAtttt                       >  1:5118266/1‑53 (MQ=255)
                                                                tGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCt                     >  1:4740237/1‑55 (MQ=255)
                                                                tGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACg     >  1:7358207/1‑71 (MQ=255)
                                                                  aTTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCAtttt                       <  1:6432335/51‑1 (MQ=255)
                                                                  aTTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGtt   <  1:4728103/71‑1 (MQ=255)
                                                                  aTTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGt    <  1:4727784/70‑1 (MQ=255)
                                                                  aTTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGt    >  1:4390581/1‑70 (MQ=255)
                                                                   tttCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGTTa  <  1:5074592/71‑1 (MQ=255)
                                                                     tcatcaATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCt                     <  1:7162021/50‑1 (MQ=255)
                                                                     |                                                                    
AATTCATTGATTAATATCAACAAGATACAAAAAGCACTATCATTAAAATTCATTGCAGTTACATTGATTTCATCAATGAAATGTAAAAATATATAAACTTGATGATTTAAGCATTTTCTTATACCCGTTCAGACGTTA  >  W3110S.gb/1988786‑1988923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: