| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,087,556 | A→G | 7.3% | V36A (GTG→GCG) | yeeE ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,087,556 | 0 | A | G | 7.3% | 303.9 / 13.2 | 110 | V36A (GTG→GCG) | yeeE | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (61/41); new base G (2/6); total (63/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
ACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAG > W3110S.gb/2087488‑2087625 | aCGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCAc < 1:7238085/70‑1 (MQ=255) cGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGa < 1:818230/71‑1 (MQ=255) cGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGa > 1:5542931/1‑71 (MQ=255) cGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGa > 1:4309517/1‑71 (MQ=255) cGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGa > 1:2077549/1‑71 (MQ=255) gCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGa > 1:5233608/1‑70 (MQ=255) cAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGata > 1:6024802/1‑71 (MQ=255) cAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGata > 1:5452548/1‑71 (MQ=255) cAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGata > 1:5199975/1‑71 (MQ=255) cAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATATTTCAc < 1:1020355/67‑1 (MQ=255) aaaaaCCCCCACGCTTTTTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGata > 1:4722941/1‑70 (MQ=255) aaaaaCCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGatat < 1:654266/71‑1 (MQ=255) aaaaaCCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGatat < 1:5923954/71‑1 (MQ=255) aaaaCCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGAtata < 1:4453449/71‑1 (MQ=255) aaCCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGTCGTAAAACATGCGATTATTTTTCACGATATACa > 1:5651195/1‑71 (MQ=255) aCCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACat < 1:7046234/71‑1 (MQ=255) cACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATcgc > 1:2102776/1‑71 (MQ=255) cACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATcgc < 1:1378156/71‑1 (MQ=255) cACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATcgc > 1:2537823/1‑71 (MQ=255) aCGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCGCGATATGTatat > 1:1396766/1‑67 (MQ=255) aCGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCGCGATATGTatat > 1:5030156/1‑67 (MQ=255) cTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa > 1:6692485/1‑70 (MQ=255) ttGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACg > 1:7046486/1‑52 (MQ=255) ttGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGa > 1:2484093/1‑53 (MQ=255) ttGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGa > 1:7921280/1‑53 (MQ=255) ttGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGa > 1:7828697/1‑53 (MQ=255) ttGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGa > 1:3263731/1‑53 (MQ=255) gTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAacca > 1:4169128/1‑71 (MQ=255) aCCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaaa > 1:1939149/1‑65 (MQ=255) aCCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaaa > 1:5124327/1‑65 (MQ=255) ccGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAaccacc < 1:6845692/70‑1 (MQ=255) cGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATcg < 1:7459437/58‑1 (MQ=255) cGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATcg < 1:2817635/58‑1 (MQ=255) cGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTg > 1:5736145/1‑71 (MQ=255) cGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTg > 1:257454/1‑71 (MQ=255) cGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTg < 1:3774565/71‑1 (MQ=255) gAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGat > 1:7194921/1‑47 (MQ=255) gAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGt > 1:7199134/1‑71 (MQ=255) gAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGt > 1:2819325/1‑71 (MQ=255) aaaTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATc > 1:5146042/1‑55 (MQ=255) aaaTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATc > 1:1767247/1‑55 (MQ=255) aaaTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATc > 1:3364101/1‑55 (MQ=255) aaaTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaaaa > 1:3039067/1‑62 (MQ=255) aaaTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaaaa > 1:4520103/1‑62 (MQ=255) aaaTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGt > 1:3811929/1‑70 (MQ=255) aaaTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc < 1:2018744/71‑1 (MQ=255) aaaTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc < 1:2883667/71‑1 (MQ=255) aaTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAacca > 1:6778944/1‑64 (MQ=255) aaTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCa < 1:7014190/71‑1 (MQ=255) aTTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCa > 1:1299336/1‑40 (MQ=255) aaTCAGCAAGGCGTAAAACATGCGATTATTTTTCa > 1:328272/1‑35 (MQ=255) aaTCAGCAAGGCGTAAAACATGCGATTATTTTTCa > 1:7039742/1‑35 (MQ=255) aaTCAGCAAGGCGTAAAACATGCGATTATTTTTCGCGATATGTATATCGTGag < 1:3432846/53‑2 (MQ=21) aaTCAGCAAGGCGTAAAACATGCGATTATTTTTCGCGATATGTATATCGTGaa < 1:3580094/53‑1 (MQ=21) aTCAGCAAGGCGTAAAACATGCGATTATTTTTCGCGatat < 1:5908502/40‑1 (MQ=255) aTCAGCAAGGCGTAAAACATGCGATTATTTTTCGCGatat < 1:4936624/40‑1 (MQ=255) aTCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCaaaa < 1:4779374/71‑1 (MQ=255) tCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc < 1:2446845/62‑1 (MQ=255) tCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCaaaaa > 1:2683292/1‑71 (MQ=255) tCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCaaaaa > 1:6795968/1‑71 (MQ=255) tCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCaaaaa > 1:6970015/1‑71 (MQ=255) cAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCt > 1:3692620/1‑58 (MQ=255) cAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCt > 1:7471150/1‑58 (MQ=255) cAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCt > 1:171007/1‑58 (MQ=255) cAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCa > 1:4315821/1‑62 (MQ=255) aGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACg > 1:1553480/1‑71 (MQ=255) aGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACg > 1:1116944/1‑71 (MQ=255) cAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGcc < 1:5422811/71‑1 (MQ=255) aaGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc < 1:7001691/57‑1 (MQ=255) aaGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc < 1:1005448/57‑1 (MQ=255) aaGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc < 1:3805575/57‑1 (MQ=255) aaGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGcc > 1:6686646/1‑70 (MQ=255) ggCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCa > 1:1677079/1‑70 (MQ=255) gCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACg < 1:1190533/71‑1 (MQ=255) cGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGc < 1:5652204/71‑1 (MQ=255) gTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACg < 1:2887553/63‑1 (MQ=255) gTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACg < 1:367258/63‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGaaaa < 1:7292087/42‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGaaaa < 1:3020556/42‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTg < 1:6234848/71‑1 (MQ=255) aaaaCATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTg > 1:6437121/1‑70 (MQ=255) aaaCATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCaaaaa > 1:7536008/1‑58 (MQ=255) aaaCATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGc < 1:3350900/70‑1 (MQ=255) aaaCATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCa > 1:2224482/1‑71 (MQ=255) aaCATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTg > 1:5061069/1‑46 (MQ=255) aaCATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTg > 1:5052103/1‑46 (MQ=255) cATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGa > 1:2107564/1‑71 (MQ=255) cATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGa > 1:7353353/1‑71 (MQ=255) tGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCa > 1:7088930/1‑45 (MQ=255) tGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCa > 1:2122344/1‑45 (MQ=255) tGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCa > 1:2558825/1‑45 (MQ=255) cGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCaaa < 1:2456208/49‑1 (MQ=255) gATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCAc < 1:6348460/57‑1 (MQ=255) attTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATcc > 1:6677578/1‑69 (MQ=255) tttttCACGATATACATATCGCGAAAACCACCTGTCAGGCaaaa > 1:597213/1‑44 (MQ=255) tttttCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcag < 1:5533037/71‑1 (MQ=255) ttttCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcag < 1:3738604/70‑1 (MQ=255) tCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcagcag < 1:569328/70‑1 (MQ=255) cACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCagag > 1:6675131/1‑71 (MQ=255) aCGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCagag < 1:1774273/70‑1 (MQ=255) | ACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAG > W3110S.gb/2087488‑2087625 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |