| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,166,086 | C→G | 9.6% | F358L (TTC→TTG) | baeS → | sensory histidine kinase in two‑component regulatory system with BaeR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,166,086 | 0 | C | G | 9.6% | 204.7 / 9.7 | 73 | F358L (TTC→TTG) | baeS | sensory histidine kinase in two‑component regulatory system with BaeR |
| Reads supporting (aligned to +/- strand): ref base C (33/33); new base G (4/3); total (37/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
GAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAATCTCTGCCGGGCAGC > W3110S.gb/2166017‑2166156 | gAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTc < 1:935868/70‑1 (MQ=255) aaaCTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTc < 1:2483291/69‑1 (MQ=255) aaCTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCaa > 1:4618909/1‑70 (MQ=255) aaCTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCaa > 1:2192413/1‑70 (MQ=255) aaCTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAAt < 1:3347698/71‑1 (MQ=255) cAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACt > 1:6239856/1‑70 (MQ=255) cAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACt > 1:5360162/1‑70 (MQ=255) ttttCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACt > 1:3967901/1‑71 (MQ=255) ttttCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACt > 1:5318924/1‑71 (MQ=255) ttCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTg > 1:4730050/1‑70 (MQ=255) ttCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTg > 1:7030763/1‑70 (MQ=255) ttCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTg > 1:5121087/1‑70 (MQ=255) tCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTgg < 1:3668325/70‑1 (MQ=255) tCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTgg < 1:5399495/70‑1 (MQ=255) ccTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTGAATAACt < 1:1014351/62‑1 (MQ=255) ccTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTGAATAACt < 1:4955852/62‑1 (MQ=255) ccTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTGAATAACt < 1:1132138/62‑1 (MQ=255) tGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTGAATAACtgc > 1:7824385/1‑60 (MQ=255) tGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTGAATAACtgc > 1:1522469/1‑60 (MQ=255) tGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTGAATAACtgc > 1:1166638/1‑60 (MQ=255) tGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaaaa > 1:4771210/1‑70 (MQ=255) tGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaa < 1:7904657/68‑1 (MQ=255) gCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAAc > 1:4057548/1‑58 (MQ=255) ccAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAg > 1:1695420/1‑71 (MQ=255) cAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGc < 1:7597285/71‑1 (MQ=255) gACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCt > 1:1091781/1‑71 (MQ=255) cAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTc > 1:35355/1‑46 (MQ=255) tATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaa < 1:7170704/58‑1 (MQ=255) ttACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTa < 1:5664580/70‑1 (MQ=255) ttACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTa > 1:3376327/1‑70 (MQ=255) tACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaa < 1:2755632/55‑1 (MQ=255) tACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaa < 1:6097063/55‑1 (MQ=255) gTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACt > 1:6815972/1‑69 (MQ=255) tttGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACt < 1:6726948/40‑1 (MQ=255) tttGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaaaa < 1:4218827/50‑1 (MQ=255) tttGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaaa > 1:6126082/1‑49 (MQ=255) tttGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACa < 1:628714/70‑1 (MQ=255) ttGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGATACACTGACAgc > 1:5134065/1‑71 (MQ=255) tGGCGATCGCGACCGTTTAATGCAGTTATTGAATAACTTa > 1:7412472/1‑40 (MQ=255) tGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTa > 1:2446147/1‑40 (MQ=255) tGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAgc < 1:5702678/70‑1 (MQ=255) ggCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTTGAAAACAGCCTGCGCTACACTGACAgcg < 1:874031/70‑1 (MQ=255) gCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAgcgg < 1:2335044/70‑1 (MQ=255) cGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaaaa > 1:7420997/1‑45 (MQ=255) cGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaaaa > 1:35130/1‑45 (MQ=255) cGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaaaa > 1:2528989/1‑45 (MQ=255) cGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaaaa > 1:3806206/1‑45 (MQ=255) cGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAgcggc > 1:5217066/1‑70 (MQ=255) gATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaaaa < 1:1175674/44‑1 (MQ=255) gATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaaaa < 1:3757411/44‑1 (MQ=255) gATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaa < 1:6864803/42‑1 (MQ=255) gATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaa < 1:5054033/42‑1 (MQ=255) gATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGaa < 1:2695043/42‑1 (MQ=255) gATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAg > 1:3855255/1‑65 (MQ=255) gATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGcggcgg < 1:6847980/71‑1 (MQ=255) cgcgACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACa < 1:5824865/43‑1 (MQ=255) cgcgACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCa < 1:4593389/70‑1 (MQ=255) cgcgACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAg > 1:7338904/1‑71 (MQ=255) cgACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGcc < 1:323712/71‑1 (MQ=255) gACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGcc < 1:3073831/43‑1 (MQ=255) gACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCt > 1:1963251/1‑71 (MQ=255) ccGTTTAATGCAGTTATTCAATAACTTACTGGaaaa > 1:74614/1‑36 (MQ=255) tttAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGcc < 1:1825160/65‑1 (MQ=255) tttAATGCAGTTATTCAATAACTTACTGGAAAACAGCCCGCGCTACACTGACAGCGGCGGCAGcc < 1:3264339/65‑1 (MQ=255) ttAATGCAGTTATTCAATAACTTACTGGAAAACAGc > 1:2035617/1‑36 (MQ=255) ttAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTaca > 1:1414423/1‑46 (MQ=255) ttAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTaca > 1:961388/1‑46 (MQ=255) ttAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAAt > 1:3589579/1‑71 (MQ=255) tAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTg > 1:3236194/1‑65 (MQ=255) aaTGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAAtc < 1:2508034/70‑1 (MQ=255) tGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAATctct < 1:6532683/71‑1 (MQ=255) ttATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAg > 1:4698342/1‑53 (MQ=255) aTTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAATCTCTGCCggg < 1:2765074/70‑1 (MQ=255) ttCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCaa < 1:2262789/57‑1 (MQ=255) ttCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAATCTCTGCCGGGc < 1:2552376/70‑1 (MQ=255) ttCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAATCTCTGCCGGGc < 1:7357825/70‑1 (MQ=255) cAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAATCTCTGCCGGGCAgc > 1:348559/1‑71 (MQ=255) cAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAATCTCTGCCGGGCAg > 1:3073350/1‑70 (MQ=255) | GAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAATCTCTGCCGGGCAGC > W3110S.gb/2166017‑2166156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |