| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,217,253 | T→C | 8.0% | E242E (GAA→GAG) | yehU ← | predicted sensory kinase in two‑component system with YehT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,217,253 | 0 | T | C | 8.0% | 342.4 / 17.8 | 125 | E242E (GAA→GAG) | yehU | predicted sensory kinase in two‑component system with YehT |
| Reads supporting (aligned to +/- strand): ref base T (75/40); new base C (4/6); total (79/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
TCGTCACCAATTCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGGCGCAAAATGCCTTCCG > W3110S.gb/2217185‑2217323 | tcgtcACCAATTCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTc > 1:1813033/1‑71 (MQ=255) aCCAATTCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGa > 1:603787/1‑70 (MQ=255) aCCAATTCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGa > 1:1890812/1‑70 (MQ=255) cAATTCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATa > 1:3244684/1‑70 (MQ=255) cAATTCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATa > 1:4740637/1‑70 (MQ=255) aaTTCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCTCCAATATCCAGtt < 1:2585905/62‑1 (MQ=255) aaTTCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGtt < 1:3606338/62‑1 (MQ=255) aTTCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAg > 1:5041161/1‑71 (MQ=255) aTTCCGGTAAAGGCCAGCAATTTATCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAg < 1:7302657/71‑1 (MQ=255) ttCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAg > 1:3120764/1‑70 (MQ=255) ccGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCAc > 1:2372314/1‑71 (MQ=255) gTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTg < 1:7279707/71‑1 (MQ=255) tAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCa < 1:1447816/66‑1 (MQ=255) tAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCt > 1:670636/1‑69 (MQ=255) aaaGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAg > 1:5958256/1‑71 (MQ=255) aaGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAg < 1:5463008/70‑1 (MQ=255) aaGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGc > 1:3931341/1‑71 (MQ=255) aaGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGc > 1:625193/1‑71 (MQ=255) aGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGcc < 1:5505816/71‑1 (MQ=255) ttaTTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCa < 1:2061122/69‑1 (MQ=255) taTTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCa < 1:10305/69‑1 (MQ=255) aaTTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCAt > 1:767633/1‑71 (MQ=255) aTTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATg > 1:7689989/1‑71 (MQ=255) tttCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGc > 1:1635648/1‑71 (MQ=255) tttCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGc > 1:7359481/1‑71 (MQ=255) tctctcGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCt < 1:6750056/70‑1 (MQ=255) ctctcGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGt > 1:1161772/1‑71 (MQ=255) tctcGATCGGTAATCGCGACTGCACCAATATCCAGCTCCTGa < 1:4123526/42‑1 (MQ=255) tctcGATCGGTAATCGCGACTGCACCAATATCCAGCTCCTGa < 1:5086546/42‑1 (MQ=255) tctcGATCGGTAATCGCGACTGCACCAATATCCAGCTCCTGa < 1:733400/42‑1 (MQ=255) tctcGATCGGTAATCGCGACTGCACCAATATCCAGCTCCTGa < 1:21986/42‑1 (MQ=255) tcGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAg < 1:796348/45‑1 (MQ=255) tcGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAg < 1:350041/45‑1 (MQ=255) tcGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGt < 1:7121721/68‑1 (MQ=255) tcGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGt < 1:4332926/68‑1 (MQ=255) cGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGtt < 1:3821016/68‑1 (MQ=255) gATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTg > 1:3650076/1‑65 (MQ=255) gATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACt < 1:1997412/71‑1 (MQ=255) gATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACt > 1:4572052/1‑71 (MQ=255) aTCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACttt < 1:6531653/57‑1 (MQ=255) tCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCAcc > 1:488890/1‑45 (MQ=255) tCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCAcc > 1:6172753/1‑45 (MQ=255) cGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTc < 1:430950/70‑1 (MQ=255) cGGTAATCGCGACTGCACCAATATCCAGCTCCTGATACAGCACCTGTGCCACTTTCATGCTg > 1:462523/1‑62 (MQ=255) cGGTAATCGCGACTGCACCAATATCCAGCTCCTGATACAGCACCTGTGCCACTTTCATGCTg > 1:1058258/1‑62 (MQ=255) cGGTAATCGCGACTGCACCAATATCCAGCTCCTGATACAGCACCTGTGCCACTTTCATGCTg > 1:1769786/1‑62 (MQ=255) cGGTAATCGCGACTGCACCAATATACAGCTCCTGATACAGCACCTGTGCCACTTTCATGCTg > 1:6520749/1‑62 (MQ=255) ggTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCg < 1:3087625/70‑1 (MQ=255) tAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTa < 1:5401149/71‑1 (MQ=255) tAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTa < 1:3211117/71‑1 (MQ=255) tAATCGCGACTGCACCAATATCCAGTTCCTGATACAGAACCTGAGCCACTTTCATg > 1:7906952/1‑56 (MQ=255) aaTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTc > 1:3339328/1‑52 (MQ=255) aaTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCa > 1:3917569/1‑53 (MQ=255) aaTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCa > 1:6219009/1‑53 (MQ=255) aaTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTa > 1:7197640/1‑70 (MQ=255) aaTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTa > 1:2890137/1‑70 (MQ=255) aaTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTa > 1:7083546/1‑70 (MQ=255) aTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAg > 1:1695732/1‑43 (MQ=255) aTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAg > 1:6800760/1‑43 (MQ=255) aTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAg > 1:6395375/1‑43 (MQ=255) aTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCa > 1:1975178/1‑52 (MQ=255) aTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGt > 1:4921835/1‑67 (MQ=255) aTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGt > 1:6594163/1‑67 (MQ=255) aTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGt > 1:1397022/1‑67 (MQ=255) aTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTaa < 1:6404503/70‑1 (MQ=255) aTCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTa < 1:4923266/69‑1 (MQ=255) cgcgACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAAcc > 1:14680/1‑71 (MQ=255) cgACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGtt > 1:5719055/1‑64 (MQ=255) cgACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAAcccc < 1:7176832/71‑1 (MQ=255) cgACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAAcccc < 1:2490012/71‑1 (MQ=255) gACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACt > 1:2837958/1‑43 (MQ=255) gACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTaaa > 1:4588513/1‑66 (MQ=255) gACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTaaa > 1:5582890/1‑66 (MQ=255) gACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAAccc > 1:5571301/1‑69 (MQ=255) gACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCt > 1:2192917/1‑71 (MQ=255) gCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCAt > 1:4753777/1‑44 (MQ=255) gCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCAt > 1:5705828/1‑44 (MQ=255) gCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCAt > 1:7848454/1‑44 (MQ=255) gCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCAt > 1:6663266/1‑44 (MQ=255) cACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGgcgc < 1:5721043/71‑1 (MQ=255) cAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCt < 1:4760772/43‑1 (MQ=255) cAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACtt > 1:730761/1‑51 (MQ=255) cAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACtt > 1:4036935/1‑51 (MQ=255) cAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCg < 1:3625304/53‑1 (MQ=255) cAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCg < 1:1663141/53‑1 (MQ=255) cAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGGCGCaaa < 1:4669912/71‑1 (MQ=255) aaTATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTa < 1:2006658/55‑1 (MQ=255) aaTATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGGCGCaaaa < 1:2331057/71‑1 (MQ=255) atatCCATTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGGCGCAAAAt > 1:1897197/1‑71 (MQ=255) atatCCAGTTCCTGATACAGCACCTGAGCCACttt > 1:559249/1‑35 (MQ=255) atatCCAGTTCCTGATACAGCACCTGAGCCACttt > 1:2655556/1‑35 (MQ=255) atatCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGt > 1:2602197/1‑43 (MQ=255) atatCAAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGt > 1:2991984/1‑43 (MQ=255) tCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACt > 1:4962950/1‑45 (MQ=255) cAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGGCGCAAAATGCCtt < 1:2426592/71‑1 (MQ=255) cAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGGCGCAAAATGCCt < 1:2708085/70‑1 (MQ=255) aGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGGCGCAAAATGCCTTc > 1:5120398/1‑71 (MQ=255) ttCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCg < 1:7078196/43‑1 (MQ=255) ttCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGGCGCAAAATGCCTTCCg < 1:792608/71‑1 (MQ=255) ttCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGGCGCAAAATGCCTTCCg < 1:1167782/71‑1 (MQ=255) | TCGTCACCAATTCCGGTAAAGGCCAGCAATTTCTCTCGATCGGTAATCGCGACTGCACCAATATCCAGTTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGGCGCAAAATGCCTTCCG > W3110S.gb/2217185‑2217323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |