| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,264,702 | G→A | 6.6% | R15C (CGC→TGC) | fruA ← | fused fructose‑specific PTS enzyme IIB'BC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,264,702 | 0 | G | A | 6.6% | 238.4 / 4.2 | 75 | R15C (CGC→TGC) | fruA | fused fructose‑specific PTS enzyme IIB'BC components |
| Reads supporting (aligned to +/- strand): ref base G (35/35); new base A (2/3); total (37/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGATTTCCAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAGTTAAAA > W3110S.gb/2264640‑2264770 | gatgatTTCCAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGa < 1:4141775/70‑1 (MQ=255)gatgatTTCCAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGAc > 1:3653201/1‑71 (MQ=255)gatgatTTCCAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGAc < 1:7439794/71‑1 (MQ=255) atgatTTCCAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGAcc > 1:4492506/1‑71 (MQ=255) tgatTTCCAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGAcc > 1:6100317/1‑70 (MQ=255) gatTTCCAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGAcc > 1:5902456/1‑69 (MQ=255) gatTTCCAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGAcc > 1:2441641/1‑69 (MQ=255) tttCCAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCgaga < 1:5473554/71‑1 (MQ=255) ccAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGAt > 1:4311285/1‑69 (MQ=255) cAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAg > 1:4729483/1‑71 (MQ=255) aGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGc < 1:4969249/71‑1 (MQ=255) ttttGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCg > 1:1980398/1‑70 (MQ=255) tGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCaa > 1:1663509/1‑71 (MQ=255) tGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCa < 1:3316384/70‑1 (MQ=255) gCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCaa > 1:1603696/1‑70 (MQ=255) cTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCaata < 1:1693363/71‑1 (MQ=255) cTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCaata < 1:1877984/71‑1 (MQ=255) tCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATc < 1:1239412/70‑1 (MQ=255) tCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATc < 1:170256/70‑1 (MQ=255) tCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATc < 1:5217976/70‑1 (MQ=255) ccgccgcGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGCCCGAGATTAGCGTCAATAATCagcagc > 1:6771098/1‑71 (MQ=255) cgccgcGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCg < 1:93309/71‑1 (MQ=255) ccgcGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGtt > 1:2635851/1‑71 (MQ=255) gcgcCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGttt > 1:1563770/1‑70 (MQ=255) gcCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCa < 1:3221659/71‑1 (MQ=255) cccAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCa < 1:7123068/51‑1 (MQ=255) agcagGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTAt > 1:5985691/1‑71 (MQ=255) agcagGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTAt < 1:3186630/71‑1 (MQ=255) agcagGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTAt < 1:690113/71‑1 (MQ=255) gcagGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTAt > 1:5691386/1‑70 (MQ=255) agGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTc > 1:5042578/1‑63 (MQ=255) gggTCTTCGCCATATAGGCGCGTGCCTGACCgaga > 1:5427990/1‑35 (MQ=255) gggTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGtttt < 1:6771731/61‑1 (MQ=255) gggTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCct > 1:3560422/1‑71 (MQ=255) gggTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCct > 1:1630486/1‑71 (MQ=255) ggTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGttt < 1:7921804/59‑1 (MQ=255) ggTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCctc > 1:2599440/1‑71 (MQ=255) tCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGc > 1:1041301/1‑37 (MQ=255) tCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGc > 1:966661/1‑37 (MQ=255) tCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGc > 1:7182977/1‑37 (MQ=255) tCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGc > 1:1060389/1‑37 (MQ=255) ttCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCaataat > 1:1338264/1‑44 (MQ=255) ttCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATca > 1:161799/1‑46 (MQ=255) ttCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATc < 1:110724/45‑1 (MQ=255) ttCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGttt < 1:2213014/55‑1 (MQ=255) ttCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGc > 1:1986659/1‑64 (MQ=255) ttCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGc > 1:2734940/1‑64 (MQ=255) cGCCATCTAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTAATTATGCCTCTCctgc > 1:1021289/1‑71 (MQ=255) cGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTcc < 1:6795009/68‑1 (MQ=255) cGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCctgc > 1:2939422/1‑71 (MQ=255) cGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCctg < 1:2846680/70‑1 (MQ=255) gCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTcc < 1:5485598/67‑1 (MQ=255) gCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTcc < 1:7844130/67‑1 (MQ=255) cATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCctg < 1:2727164/67‑1 (MQ=255) atatAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGt > 1:7714674/1‑70 (MQ=255) atatAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGt > 1:3499175/1‑70 (MQ=255) atatAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATCATGCCTCTCCTGCTGt > 1:5404315/1‑70 (MQ=255) tataGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTa < 1:6143712/54‑1 (MQ=255) tataGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGt > 1:6015544/1‑69 (MQ=255) tataGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCa < 1:4717328/71‑1 (MQ=255) tataGGCGCATGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGttt < 1:3983888/48‑1 (MQ=255) tataGGCGCATGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGttt < 1:3611015/48‑1 (MQ=255) tataGGCGCATGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGttt < 1:3486722/48‑1 (MQ=255) ataGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAg > 1:5902590/1‑71 (MQ=255) taGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCAt < 1:6109418/50‑1 (MQ=255) taGGCGCATGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTc > 1:1419169/1‑48 (MQ=255) taGGCGCATGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTc > 1:7774525/1‑48 (MQ=255) aGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCAt < 1:7205686/49‑1 (MQ=255) aGGCGCGCGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCAt < 1:5615971/49‑1 (MQ=255) ggCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAGTTa < 1:3397454/71‑1 (MQ=255) ggCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAGTTa < 1:1472563/71‑1 (MQ=255) ggCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAGTTa < 1:3599954/71‑1 (MQ=255) gcgcGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCctgctg < 1:7753159/63‑1 (MQ=255) gcgcGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTc < 1:7177319/65‑1 (MQ=255) gcgcGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAGTTaa < 1:432147/71‑1 (MQ=255) gcgTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAg > 1:4971842/1‑65 (MQ=255) gcgTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAg > 1:1768344/1‑65 (MQ=255) gcgTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAg > 1:6638726/1‑65 (MQ=255) cgTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCAt < 1:5316446/44‑1 (MQ=255) gTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAGTTaaaa > 1:7668538/1‑69 (MQ=255) | GATGATTTCCAGTTTTGCTTTTCGCGCCGCCGCGCCCAGCAGGGTCTTCGCCATATAGGCGCGTGCCTGACCGAGATTAGCGTCAATAATCAGCAGCGTTTTCATTATGCCTCTCCTGCTGTCAGTTAAAA > W3110S.gb/2264640‑2264770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |