| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 143,730 | A→G | 8.1% | K10R (AAA→AGA) | yadH → | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 143,730 | 0 | A | G | 8.1% | 237.0 / 11.0 | 86 | K10R (AAA→AGA) | yadH | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (37/42); new base G (3/4); total (40/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
TTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCA > W3110S.gb/143664‑143798 | tttgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaaaa < 1:2682988/69‑1 (MQ=255)tttgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaaaa < 1:2689941/69‑1 (MQ=255)tttgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaaaa < 1:6524649/69‑1 (MQ=255)tttgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaaaa < 1:2929072/69‑1 (MQ=255) ttgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaaaa > 1:6341075/1‑68 (MQ=255) ttgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCa > 1:2466149/1‑71 (MQ=255) tgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaaaa > 1:880725/1‑67 (MQ=255) tgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaaaa > 1:1590277/1‑67 (MQ=255) tgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaaaa > 1:514614/1‑67 (MQ=255) tgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaaa > 1:5056231/1‑66 (MQ=255) tgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCa < 1:3419128/70‑1 (MQ=255) tgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCa < 1:3729989/70‑1 (MQ=255) tCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTg < 1:2415642/70‑1 (MQ=255) cACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTg < 1:847099/69‑1 (MQ=255) aCTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaa < 1:2909784/59‑1 (MQ=255) cTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAATGCATCt < 1:1027684/66‑1 (MQ=255) gTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGaaa < 1:5384600/71‑1 (MQ=255) gTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGaaa < 1:7068453/71‑1 (MQ=255) gTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGa > 1:4136993/1‑69 (MQ=255) gTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGa > 1:4079042/1‑69 (MQ=255) ttAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCg < 1:3618231/67‑1 (MQ=255) ttAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAag < 1:7213145/71‑1 (MQ=255) aTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGaaa > 1:5582665/1‑67 (MQ=255) aTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGaaa > 1:1036304/1‑67 (MQ=255) aTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGaaa > 1:1746213/1‑67 (MQ=255) aTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGaaa > 1:505351/1‑67 (MQ=255) aTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAagag < 1:2183928/70‑1 (MQ=255) aTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAagag < 1:32712/70‑1 (MQ=255) aTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAagag < 1:2908273/70‑1 (MQ=255) tGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTaaa < 1:4690591/50‑1 (MQ=255) aaaaaCAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATc > 1:3373581/1‑70 (MQ=255) aaaaCAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATc < 1:6095515/54‑1 (MQ=255) aaaaCAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATc < 1:5373668/54‑1 (MQ=255) aaaaCAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATcc < 1:2475385/70‑1 (MQ=255) aCAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCAt < 1:4929932/69‑1 (MQ=255) gagaTCGCGCATGATGCATCTTTACTGGGTGGCGCTaaa < 1:1909776/39‑1 (MQ=255) agaTCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCTCTTTAt < 1:1016655/71‑1 (MQ=255) cgcgCATGATGCCTCTTTACTGGGTGGCGCTAAAAAGCATc > 1:5533824/1‑41 (MQ=255) cgcgCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATc > 1:6460151/1‑41 (MQ=255) cgcgCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTa > 1:2013333/1‑66 (MQ=255) gcgcATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCAt < 1:6969702/58‑1 (MQ=255) gcgcATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCAt < 1:7116199/58‑1 (MQ=255) cgcATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTAt > 1:5341487/1‑71 (MQ=255) cgcATGATGCATATTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTa > 1:6717321/1‑70 (MQ=255) cacATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTAt > 1:4476485/3‑71 (MQ=255) gcATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATc > 1:163868/1‑71 (MQ=255) tgatgCATCTTTACTGGGTGGCGCTAAAAAGCATCTgg < 1:4751188/38‑1 (MQ=255) gCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGa < 1:3546780/38‑1 (MQ=255) gCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGa < 1:5070044/38‑1 (MQ=255) gCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGa < 1:1718563/38‑1 (MQ=255) gCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGa < 1:6813956/38‑1 (MQ=255) gCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCtt < 1:4984478/54‑1 (MQ=255) gCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTa > 1:2836473/1‑56 (MQ=255) cATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTg > 1:309890/1‑69 (MQ=255) cATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTg > 1:7626071/1‑69 (MQ=255) cATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCa < 1:3128150/71‑1 (MQ=255) cATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATAGCTTTATGCGTATCTGGGTg > 1:7377436/1‑69 (MQ=255) aTCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTg > 1:3837023/1‑68 (MQ=255) tCTTTACTGGGTGGCGCTAAGAAGCATCTGGGCGAAAGAGATCCATCGCtt < 1:4383092/51‑1 (MQ=255) tCTTTACTGGGTGGCGCTAAGAAGCATCTGGGCGAAAGAGATCCATCGCtt < 1:1894642/51‑1 (MQ=255) tCTTTACTGGGTGGCGCTAAGAAGCATCTGGGCGAAAGAGATCCATCGCtt < 1:5101532/51‑1 (MQ=255) tCTTTACTGGGTGGCGCTAAGAAGCATCTGGGCGAAAGAGATCCATCGCtt < 1:5110746/51‑1 (MQ=255) cTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGAc > 1:6712684/1‑71 (MQ=255) tttACTGGGTGGCGCTAAGAAGCATCTGGGCGAAAGAGATCCATCGCTTTa > 1:7248957/1‑51 (MQ=255) tttACTGGGTGGCGCTAAGAAGCATCTGGGCGAAAGAGATCCATCGCTTTa > 1:822162/1‑51 (MQ=255) tttACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGGGATCCATCGCTTTATGCGTATCTGGGt > 1:532521/1‑64 (MQ=255) tttACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACg > 1:3876858/1‑71 (MQ=255) tttACTAGGTGGCGCTAAGAAGCATCTGGGCGAAAGAGATCCATCGCTTTa > 1:16563/1‑51 (MQ=255) tACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAgaga > 1:4147366/1‑37 (MQ=255) tACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCttt < 1:3708205/48‑1 (MQ=255) tACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACg < 1:1597836/69‑1 (MQ=255) tACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGc < 1:6789449/70‑1 (MQ=255) tACTGGGTGGCGCTAAAAAGCATATGGGCGAAAGAGATCCATCGc > 1:389442/1‑45 (MQ=255) aCTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCt < 1:636502/70‑1 (MQ=255) tGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCt < 1:1162060/43‑1 (MQ=255) ggTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTa < 1:3843966/50‑1 (MQ=255) ggTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGt < 1:4762774/69‑1 (MQ=255) ggTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTg > 1:235508/1‑70 (MQ=255) tGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCg < 1:2758321/46‑1 (MQ=255) tGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATc > 1:801379/1‑50 (MQ=255) tGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATc > 1:1865502/1‑50 (MQ=255) tGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATc > 1:1289934/1‑50 (MQ=255) tGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTgg < 1:2932357/53‑1 (MQ=255) gcgcTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGc < 1:388240/43‑1 (MQ=255) gcgcTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTgccgc > 1:3452936/1‑70 (MQ=255) gcTAAAAAGCATCTGGGCGAAAGAGATCCATCGCttt < 1:430559/37‑1 (MQ=255) cTAAAAAGCATCTGGGCGAAAGAGATCCATCGCttt < 1:2592065/36‑1 (MQ=255) cTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCt < 1:2229205/46‑1 (MQ=255) cTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCt < 1:215461/46‑1 (MQ=255) cTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGt > 1:699209/1‑71 (MQ=255) cTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGt > 1:685111/1‑71 (MQ=255) cTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGt > 1:910462/1‑71 (MQ=255) tAAAAAGCATCTGGGCGAAAGAGATCCATCGCttt > 1:5654395/1‑35 (MQ=255) tAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGt > 1:3805310/1‑70 (MQ=255) aaaaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGtca > 1:1412364/1‑71 (MQ=255) aaaaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGtca > 1:6381994/1‑71 (MQ=255) aaaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCa > 1:6454940/1‑66 (MQ=255) aaaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCa > 1:2052823/1‑66 (MQ=255) aaaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGtca < 1:7388809/70‑1 (MQ=255) aaaaGCATCAGGGCGAAAGAGATCCATCGCTTTATGCGt > 1:3683582/1‑39 (MQ=255) | TTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCA > W3110S.gb/143664‑143798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |