Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,274,044 G→T 6.2% intergenic (+165/‑16) spr → / → rtn predicted peptidase, outer membrane lipoprotein/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,274,0440GT 6.2% 315.0 / 3.4 96intergenic (+165/‑16)spr/rtnpredicted peptidase, outer membrane lipoprotein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (36/54);  new base T (2/4);  total (38/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01

Only 100 of 101 total aligned reads displayed.

TGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTG  >  W3110S.gb/2273977‑2274114
                                                                   |                                                                      
tgTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGaa                                                                     <  1:6115366/71‑1 (MQ=255)
  tttCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAt                                                                   <  1:2286003/71‑1 (MQ=255)
    tCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAtt                                                                  <  1:6842769/70‑1 (MQ=255)
    tCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAtt                                                                  <  1:1994763/70‑1 (MQ=255)
     ccGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTa                                                                 <  1:4470531/70‑1 (MQ=255)
     ccGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTa                                                                 <  1:5046213/70‑1 (MQ=255)
      cgcgTCTTGATCCAGGCTATAGTCCTGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCCTGAAATTac                                                                <  1:7439503/70‑2 (MQ=255)
      cgcgTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCCTGAAATTac                                                                <  1:6295872/70‑2 (MQ=255)
      cgcgTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTaa                                                                >  1:4597470/1‑70 (MQ=255)
      cgcgTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTaa                                                                >  1:4758412/1‑70 (MQ=255)
        cgTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACgg                                                             <  1:2369278/71‑1 (MQ=255)
              gATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCAt                                                       >  1:4156849/1‑71 (MQ=255)
                tCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGt                                                     <  1:3764148/71‑1 (MQ=255)
                 ccAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGtt                                                    >  1:513698/1‑71 (MQ=255)
                  cAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGtt                                                    <  1:5773777/70‑1 (MQ=255)
                  cAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTc                                                   <  1:319221/71‑1 (MQ=255)
                  cAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTc                                                   <  1:3514873/71‑1 (MQ=255)
                   aGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                                                  <  1:5424570/71‑1 (MQ=255)
                   aGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                                                  <  1:7483148/71‑1 (MQ=255)
                   aGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                                                  <  1:6949277/71‑1 (MQ=255)
                    ggCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCAt                                                 <  1:2711209/71‑1 (MQ=255)
                    ggCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCAt                                                 <  1:4389391/71‑1 (MQ=255)
                    ggCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATCTTCa                                                  >  1:3123887/1‑70 (MQ=255)
                          aGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCATGAAATTAACGGAATCATGTTCATACgcgc                                           >  1:6629142/1‑71 (MQ=255)
                          aGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACgcgc                                           >  1:5285612/1‑71 (MQ=255)
                           gTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                                                        >  1:1163228/1‑41 (MQ=255)
                           gTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGa                                                                      >  1:7204043/1‑43 (MQ=255)
                           gTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCt                                          >  1:620130/1‑71 (MQ=255)
                             ccGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCt                                          >  1:1959410/1‑69 (MQ=255)
                              cGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTccc                                       <  1:789447/71‑1 (MQ=255)
                                gTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCaa                                     <  1:3174955/71‑1 (MQ=255)
                                  cATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                                                  <  1:7649458/56‑1 (MQ=255)
                                  cATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                                                  <  1:2885322/56‑1 (MQ=255)
                                  cATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                                                  <  1:620117/56‑1 (MQ=255)
                                  cATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc                                               <  1:5576686/59‑1 (MQ=255)
                                  cATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt                                   >  1:5259627/1‑71 (MQ=255)
                                   aTTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc                                               >  1:2124259/1‑58 (MQ=255)
                                   aTTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc                                               >  1:2114238/1‑58 (MQ=255)
                                   aTTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc                                               >  1:7839092/1‑58 (MQ=255)
                                   aTTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt                                   <  1:6920075/70‑1 (MQ=255)
                                    ttGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                 <  1:5683586/71‑1 (MQ=255)
                                    ttGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                 <  1:1447719/71‑1 (MQ=255)
                                     tGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAt                                                                   >  1:4253634/1‑36 (MQ=255)
                                     tGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAt                                                                   >  1:6784979/1‑36 (MQ=255)
                                      gTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTg                                <  1:3183999/70‑1 (MQ=255)
                                       ttATCTTTTAAATGTTGTCGTAATTTCAGGAAATTaa                                                                >  1:4729600/1‑37 (MQ=255)
                                         aTCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                 >  1:6845675/1‑66 (MQ=255)
                                         aTCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                 >  1:2881823/1‑66 (MQ=255)
                                         aTCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                 >  1:5170401/1‑66 (MQ=255)
                                         aTCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAc                             <  1:7472594/70‑1 (MQ=255)
                                         aTCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAc                             <  1:767917/70‑1 (MQ=255)
                                         aTCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAc                             <  1:2598593/70‑1 (MQ=255)
                                          tCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGt                                                     <  1:5769194/45‑1 (MQ=255)
                                          tCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGt                                                     <  1:476813/45‑1 (MQ=255)
                                          tCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACg                            >  1:3594337/1‑70 (MQ=255)
                                           cTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGt                                                     <  1:876478/44‑1 (MQ=255)
                                           cTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt                                   <  1:3707800/62‑1 (MQ=255)
                                           cTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGt                           >  1:3672922/1‑70 (MQ=255)
                                             tttAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                 >  1:5177136/1‑62 (MQ=255)
                                              ttAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGt                           <  1:2629087/67‑1 (MQ=255)
                                              ttAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGt                           <  1:7611228/67‑1 (MQ=255)
                                                 aaTGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTcc                    <  1:6400536/71‑1 (MQ=255)
                                                 aaTGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTcc                    <  1:4545987/71‑1 (MQ=255)
                                                 aaTGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTc                     <  1:4599784/70‑1 (MQ=255)
                                                   tgttgtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTg                  <  1:4922830/71‑1 (MQ=255)
                                                    gttgtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt                                   <  1:1730648/53‑1 (MQ=255)
                                                    gttgtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt                                   <  1:4806729/53‑1 (MQ=255)
                                                    gttgtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt                                   <  1:4565540/53‑1 (MQ=255)
                                                     ttgtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAt                                    <  1:4106027/51‑1 (MQ=255)
                                                     ttgtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCt                <  1:820237/71‑1 (MQ=255)
                                                     ttgtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCt                <  1:7734828/71‑1 (MQ=255)
                                                      tgtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTc                     >  1:6196153/1‑65 (MQ=255)
                                                      tgtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCt                >  1:821747/1‑70 (MQ=255)
                                                      tgtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCt                >  1:3936243/1‑70 (MQ=255)
                                                       gtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                 <  1:3116915/52‑1 (MQ=255)
                                                       gtCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                 <  1:3343401/52‑1 (MQ=255)
                                                        tCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGc                       <  1:792386/61‑1 (MQ=255)
                                                        tCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCtttc             >  1:6421323/1‑69 (MQ=255)
                                                         gttAATTTCCTGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGc                 >  1:2604341/3‑66 (MQ=255)
                                                         cGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgcg                                            >  1:3824091/1‑39 (MQ=255)
                                                         cGTAATTTCAGGAAATTAACGGAATCATGTTCATACgcgc                                           >  1:6991760/1‑40 (MQ=255)
                                                         cGTAATTTCAGGAAATTAACGGAATCATGTTCATACgcgc                                           >  1:7896573/1‑40 (MQ=255)
                                                          ttAATTTCCTGAAATTAACGGAATCATGTTCATACGCGCTccc                                       <  1:5507426/42‑1 (MQ=37)
                                                          ttAATTTCCTGAAATTAACGGAATCATGTTCATACGCGCTccc                                       <  1:3590521/42‑1 (MQ=37)
                                                          gTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCt           >  1:3949825/1‑71 (MQ=255)
                                                            aaTTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTg          >  1:2131222/1‑70 (MQ=255)
                                                             aTTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCa        >  1:7781477/1‑71 (MQ=255)
                                                              tttCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCt                <  1:4911819/62‑1 (MQ=255)
                                                                 cAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                 <  1:3108198/42‑1 (MQ=255)
                                                                 cAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgt    >  1:734124/1‑71 (MQ=255)
                                                                 cAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgt    >  1:356540/1‑71 (MQ=255)
                                                                  aGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCt                <  1:7504315/58‑1 (MQ=255)
                                                                  aGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCt                <  1:5450715/58‑1 (MQ=255)
                                                                  aGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCt                <  1:3463445/58‑1 (MQ=255)
                                                                  aGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgtt   >  1:3888754/1‑71 (MQ=255)
                                                                  aGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgt    <  1:4010593/70‑1 (MQ=255)
                                                                   ggAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTg                                <  1:6785784/41‑1 (MQ=255)
                                                                   ggAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgttg  <  1:3371763/71‑1 (MQ=255)
                                                                   ggAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgttg  >  1:6707604/1‑71 (MQ=255)
                                                                   ggAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCATGCTTACCTGCAttgttg  <  1:6292138/71‑1 (MQ=255)
                                                                   |                                                                      
TGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTG  >  W3110S.gb/2273977‑2274114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: