Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,489,358 T→A 6.7% V53D (GTC→GAC)  evgA → DNA‑binding response regulator in two‑component regulatory system with EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,489,3580TA 6.7% 338.1 / 13.5 119V53D (GTC→GAC) evgADNA‑binding response regulator in two‑component regulatory system with EvgS
Reads supporting (aligned to +/- strand):  ref base T (50/61);  new base A (6/2);  total (56/63)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 127 total aligned reads displayed.

GAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTAT  >  W3110S.gb/2489288‑2489427
                                                                      |                                                                     
gagTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                       >  1:1273165/1‑71 (MQ=255)
 agTTGACTGAAGGCGGAAGTGACGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                      <  1:5626881/71‑1 (MQ=255)
  gTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACg                                                                     <  1:464722/71‑1 (MQ=38)
   ttGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGa                                                                    <  1:2064304/71‑1 (MQ=255)
     gACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGata                                                                  >  1:4677162/1‑71 (MQ=255)
     gACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGat                                                                   <  1:7409986/70‑1 (MQ=38)
          aaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATcccc                                                             >  1:7634015/1‑71 (MQ=255)
           aGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCg                                                                     >  1:7915152/1‑62 (MQ=255)
           aGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                            <  1:6153389/71‑1 (MQ=255)
           aGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                            <  1:763777/71‑1 (MQ=255)
            ggCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                      >  1:3596596/1‑60 (MQ=255)
            ggCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                      >  1:889143/1‑60 (MQ=255)
              cGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                         >  1:725192/1‑71 (MQ=255)
               ggAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGt                                                        >  1:3653675/1‑71 (MQ=255)
                gAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGtt                                                       <  1:3577328/71‑1 (MQ=255)
                  aGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTaa                                                     <  1:2397142/71‑1 (MQ=255)
                    tGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACg                                                   >  1:2229180/1‑71 (MQ=255)
                    tGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACg                                                   >  1:6678226/1‑71 (MQ=255)
                    tGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACg                                                   <  1:7065737/71‑1 (MQ=255)
                      ccGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                       >  1:6779751/1‑49 (MQ=255)
                      ccGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACgg                                                  >  1:81930/1‑70 (MQ=255)
                      ccGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACgg                                                  >  1:4015880/1‑70 (MQ=255)
                       cGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTa                                                <  1:3988601/71‑1 (MQ=255)
                        gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTAt                                               >  1:7688568/1‑71 (MQ=255)
                         ttCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                            <  1:2204666/57‑1 (MQ=255)
                         ttCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATc                                              >  1:1408298/1‑71 (MQ=255)
                          tCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTAt                                               >  1:1282143/1‑69 (MQ=255)
                           cAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATc                                              >  1:4874795/1‑69 (MQ=255)
                            aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCa                                            <  1:6643950/70‑1 (MQ=255)
                            aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCa                                            <  1:243593/70‑1 (MQ=255)
                            aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAg                                           <  1:5480913/71‑1 (MQ=255)
                            aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAg                                           <  1:6780816/71‑1 (MQ=255)
                            aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAg                                           <  1:2965078/71‑1 (MQ=255)
                               gggTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                       <  1:39638/40‑1 (MQ=255)
                               gggTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                       <  1:5513309/40‑1 (MQ=255)
                               gggTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                       <  1:7502574/40‑1 (MQ=255)
                                ggTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGatat                                                                 >  1:56107/1‑45 (MQ=255)
                                ggTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtgt                                       <  1:504805/71‑1 (MQ=255)
                                 gTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAg                                           >  1:2384557/1‑66 (MQ=255)
                                 gTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGtt                                      <  1:3721548/71‑1 (MQ=255)
                                    gAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                      <  1:4837573/36‑1 (MQ=255)
                                     aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATcccc                                                             >  1:5641688/1‑44 (MQ=255)
                                     aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCgg                                                           <  1:7188100/46‑1 (MQ=255)
                                     aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCgg                                                           <  1:1364801/46‑1 (MQ=255)
                                     aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCgg                                                           <  1:5787609/46‑1 (MQ=255)
                                     aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                            <  1:7429456/45‑1 (MQ=255)
                                     aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                            <  1:5819089/45‑1 (MQ=255)
                                     aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                            <  1:5996564/45‑1 (MQ=255)
                                     aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                            <  1:7326475/45‑1 (MQ=255)
                                     aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGa                                   >  1:4886824/1‑70 (MQ=255)
                                     aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGa                                   >  1:2845669/1‑70 (MQ=255)
                                         acTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtgt                                       <  1:5570303/62‑1 (MQ=255)
                                         acTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGc                              <  1:3055505/71‑1 (MQ=255)
                                          cTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACg                               <  1:951027/69‑1 (MQ=255)
                                           ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTa                                     >  1:5010148/1‑62 (MQ=255)
                                           ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTa                                     >  1:4937528/1‑62 (MQ=255)
                                           ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaa                                  >  1:1451671/1‑65 (MQ=255)
                                           ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCt                             >  1:1925953/1‑70 (MQ=255)
                                             aaGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaa                                  >  1:1513941/1‑63 (MQ=255)
                                             aaGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaa                                  >  1:1892889/1‑63 (MQ=255)
                                              aGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTg                            <  1:835661/68‑1 (MQ=255)
                                              aGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGa                           <  1:676632/69‑1 (MQ=255)
                                                ccTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGaa                       <  1:6162183/71‑1 (MQ=255)
                                                  tGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGa                        >  1:6300412/1‑68 (MQ=255)
                                                  tGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgc                     >  1:4332248/1‑71 (MQ=255)
                                                   gATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcg                    >  1:6556906/1‑71 (MQ=255)
                                                     tatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcgc                   <  1:6412764/70‑1 (MQ=255)
                                                        cGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGtt                                      >  1:3457096/1‑48 (MQ=255)
                                                         gacatcaaTGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaaa                                 >  1:3257074/3‑52 (MQ=37)
                                                         gacatcaaTGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaaa                                 >  1:1221272/3‑52 (MQ=37)
                                                         gacatcaaTGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaaa                                 >  1:3898764/3‑52 (MQ=37)
                                                         gTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGa                           >  1:515342/1‑58 (MQ=255)
                                                          tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaa                                  >  1:6394470/1‑50 (MQ=255)
                                                          tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCt                             <  1:4590220/55‑1 (MQ=255)
                                                          tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCt                             <  1:7001987/55‑1 (MQ=255)
                                                          tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCt                             <  1:2962130/55‑1 (MQ=255)
                                                          tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGaa                       <  1:6397533/61‑1 (MQ=255)
                                                          tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGaa                       <  1:2403540/61‑1 (MQ=255)
                                                          tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAatat             <  1:77391/71‑1 (MQ=255)
                                                          tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAatat             <  1:2229998/71‑1 (MQ=255)
                                                           catcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAgg                                          >  1:6106238/1‑41 (MQ=255)
                                                            atcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGa                           >  1:2620765/1‑55 (MQ=255)
                                                            atcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGa                           >  1:1694073/1‑55 (MQ=255)
                                                            atcaaTGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAg           >  1:5787090/1‑71 (MQ=255)
                                                             tcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaa                                  <  1:7007780/47‑1 (MQ=255)
                                                             tcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaa                                  <  1:925203/47‑1 (MQ=255)
                                                             tcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaa                                  <  1:4793160/47‑1 (MQ=255)
                                                             tcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaa                                  <  1:5367272/47‑1 (MQ=255)
                                                               atTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTa                                     >  1:5002453/1‑42 (MQ=255)
                                                                  gATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAat     >  1:4316088/1‑71 (MQ=255)
                                                                   aTGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                        <  1:198185/35‑1 (MQ=255)
                                                                   aTGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                        <  1:2354974/35‑1 (MQ=255)
                                                                   aTGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                        <  1:7824298/35‑1 (MQ=255)
                                                                   aTGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                        <  1:6963171/35‑1 (MQ=255)
                                                                   aTGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGa                        <  1:1697688/51‑1 (MQ=255)
                                                                   aTGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGa                        <  1:3240407/51‑1 (MQ=255)
                                                                    tGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAatta   <  1:1023153/71‑1 (MQ=255)
                                                                     gTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattat  >  1:7244480/1‑71 (MQ=255)
                                                                      tCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCGATATAGCGGAattat  <  1:5839880/70‑1 (MQ=255)
                                                                      tCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattat  >  1:439515/1‑70 (MQ=255)
                                                                      |                                                                     
GAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTAT  >  W3110S.gb/2489288‑2489427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: