Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,939,937 T→A 5.3% L117F (TTA→TTT ygdD ← conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,939,9370TA 5.3% 317.5 / 7.1 112L117F (TTA→TTTygdDconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (67/39);  new base A (2/4);  total (69/43)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 120 total aligned reads displayed.

CCGGACGGCACAGCAATACAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCCACAAACGCAGAT  >  W3110S.gb/2939869‑2940007
                                                                    |                                                                      
ccGGACGGCACAGCAATACAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTa                                                                       <  1:6437131/70‑1 (MQ=255)
          caGCAATACAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGcc                                                            >  1:2770408/1‑71 (MQ=255)
           aGCAATACAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACAt                                                                   <  1:7616013/63‑1 (MQ=255)
           aGCAATACAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGcc                                                            >  1:985096/1‑70 (MQ=255)
           aGCAATACAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGcc                                                            >  1:5378455/1‑70 (MQ=255)
           aGCAATACAACCTTATTCATGACTTAAGCCCTTGCGCTTTAAACGGATAGCACCAACTAAc                                                                     >  1:576051/1‑61 (MQ=255)
            gCAATACAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCa                                                          <  1:7932910/71‑1 (MQ=255)
              aaTACAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTa                                                                       <  1:2519703/56‑1 (MQ=255)
               aTACAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGcc                                                       >  1:4371019/1‑71 (MQ=255)
                  cAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACa                                                                    >  1:4327297/1‑55 (MQ=255)
                  cAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCcgc                                                    <  1:2204634/71‑1 (MQ=255)
                   aaCCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCcgcg                                                   <  1:7419710/71‑1 (MQ=255)
                   aaCCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCcg                                                     >  1:3850617/1‑69 (MQ=255)
                     ccTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACa                                                                    <  1:6996534/52‑1 (MQ=255)
                     ccTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAg                                                 >  1:7270183/1‑71 (MQ=255)
                       ttATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACAtt                                                                  >  1:841542/1‑52 (MQ=255)
                       ttATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACAtt                                                                  >  1:4865014/1‑52 (MQ=255)
                          ttCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACAtt                                                                  <  1:2650744/49‑1 (MQ=255)
                          ttCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACAtt                                                                  <  1:3163542/49‑1 (MQ=255)
                          ttCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACAtt                                                                  <  1:534584/49‑1 (MQ=255)
                          ttCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACAtt                                                                  <  1:1372428/49‑1 (MQ=255)
                           tCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCt                                           >  1:6741357/1‑71 (MQ=255)
                            cATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACt                                                                        <  1:7153733/41‑1 (MQ=255)
                            cATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACt                                                                        <  1:7162757/41‑1 (MQ=255)
                            cATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCt                                           >  1:4609000/1‑70 (MQ=255)
                             aTGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTaa                                                                      >  1:5744693/1‑42 (MQ=255)
                             aTGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTaa                                                                      >  1:5757097/1‑42 (MQ=255)
                             aTGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCa                                         <  1:4523910/71‑1 (MQ=255)
                              tGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAAc                                                                     >  1:3261073/1‑42 (MQ=255)
                              tGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAAc                                                                     >  1:4808558/1‑42 (MQ=255)
                              tGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAAc                                                                     >  1:2262374/1‑42 (MQ=255)
                              tGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAAc                                                                     >  1:4284101/1‑42 (MQ=255)
                              tGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGa                                               >  1:7612086/1‑64 (MQ=255)
                              tGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCAc                                        <  1:5477496/71‑1 (MQ=255)
                               gACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCAcg                                       >  1:6613842/1‑71 (MQ=255)
                                 cTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCAcgc                                      >  1:2838495/1‑70 (MQ=255)
                                   tACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTaa                                                                >  1:3211484/1‑42 (MQ=255)
                                   tACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTaa                                                                >  1:2441242/1‑42 (MQ=255)
                                   tACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTaa                                                                >  1:4254693/1‑42 (MQ=255)
                                   tACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTaa                                                                >  1:4203845/1‑42 (MQ=255)
                                   tACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTaa                                                                >  1:4092612/1‑42 (MQ=255)
                                   tACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTa                                                                 >  1:3338436/1‑41 (MQ=255)
                                    aCGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGa                                               >  1:7730104/1‑58 (MQ=255)
                                    aCGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCAcgccgc                                   >  1:6864718/1‑70 (MQ=255)
                                    aCGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCAcgccgc                                   >  1:3655310/1‑70 (MQ=255)
                                    aCGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCAcgccgc                                   >  1:2815713/1‑70 (MQ=255)
                                    aCGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCAcgccgc                                   >  1:1024105/1‑70 (MQ=255)
                                     cGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCa                                 <  1:2476561/71‑1 (MQ=255)
                                         cTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCg                             <  1:5631728/71‑1 (MQ=255)
                                         cTTGCGCTTTAAACGGATAGCACCAACAAACATTAACGCCCAGccc                                                      <  1:3422548/46‑1 (MQ=255)
                                         cTTGCGCTTTAAACGGATAGCACCAACAAACATTAACGCCCAGccc                                                      <  1:6783747/46‑1 (MQ=255)
                                         cTTGCGCTTTAAACGGATAGCACCAACAAACATTAACGCCCAGccc                                                      <  1:6121211/46‑1 (MQ=255)
                                         cTTGCGCTTTAAACGGATAGCACCAACAAACATTAACGCCCAGccc                                                      <  1:3242457/46‑1 (MQ=255)
                                          ttGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAg                                             <  1:7047548/54‑1 (MQ=255)
                                           tGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCAggccg                                    >  1:4423890/1‑62 (MQ=255)
                                           tGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCAc                                        >  1:6293844/1‑58 (MQ=255)
                                           tGCGCTTTAAACGGATAGCACCAACAAACATTAACGCCCAGCCcgc                                                    >  1:819987/1‑46 (MQ=255)
                                           tGCGCTTTAAACGGATAGCACCAACAAACATTAACGCCCAGCCcgc                                                    >  1:810473/1‑46 (MQ=255)
                                               cTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTc                                          <  1:2970292/52‑1 (MQ=255)
                                                tttAAACGGATAGCACCAACTAACATTAACGCCCAGCCcgc                                                    <  1:2934840/41‑1 (MQ=255)
                                                tttAAACGGATAGCACCAACTAACATTAACGCCCAGCCcgc                                                    <  1:2071152/41‑1 (MQ=255)
                                                 ttAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCt                                           <  1:341217/49‑1 (MQ=255)
                                                 ttAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGAc                      >  1:1354377/1‑70 (MQ=255)
                                                  tAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAgg                                                >  1:142709/1‑43 (MQ=255)
                                                  tAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAgg                                                >  1:7882586/1‑43 (MQ=255)
                                                  tAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGTAAGCTCACGCCGCCAACCGGAGTGACa                     >  1:5769152/1‑70 (MQ=255)
                                                  tAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTg                        >  1:2782321/1‑67 (MQ=255)
                                                  tAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACa                     <  1:4503394/70‑1 (MQ=255)
                                                   aaaCGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACaa                    <  1:6247473/70‑1 (MQ=255)
                                                     aCGGATAGCACCAACTAACATAAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACg                 >  1:562017/1‑71 (MQ=255)
                                                      cGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAgg                                                >  1:5305420/1‑39 (MQ=255)
                                                      cGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAgg                                                >  1:2268798/1‑39 (MQ=255)
                                                       ggATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCa                                         >  1:6189629/1‑45 (MQ=255)
                                                       ggATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCa                                         >  1:3889596/1‑45 (MQ=255)
                                                       ggATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCa                                         >  1:7310620/1‑45 (MQ=255)
                                                       ggATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGcc               >  1:3985508/1‑71 (MQ=255)
                                                       ggATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGcc               >  1:5629966/1‑71 (MQ=255)
                                                       ggATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGcc               >  1:70361/1‑71 (MQ=255)
                                                          tAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCcac            <  1:5050419/71‑1 (MQ=255)
                                                          tAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCcac            <  1:1501804/71‑1 (MQ=255)
                                                          tAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCca             >  1:5587645/1‑70 (MQ=255)
                                                            gCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACa                     <  1:3805908/60‑1 (MQ=255)
                                                            gCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCcaca           <  1:5134199/70‑1 (MQ=255)
                                                            gCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCcaca           <  1:2123516/70‑1 (MQ=255)
                                                             cACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCgg                            <  1:263577/52‑1 (MQ=255)
                                                             cACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCgg                            <  1:4480899/52‑1 (MQ=255)
                                                             cACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCgg                            <  1:3613308/52‑1 (MQ=255)
                                                               ccAACTAACATTAACGCCCAGCCCGCGAGGAAGGTCACGCCGCCAACCGGAGTGACAAACGCCCACaa          <  1:7786162/68‑1 (MQ=255)
                                                                cAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCgg                            <  1:2525724/49‑1 (MQ=255)
                                                                cAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCCACAAACg       >  1:2582828/1‑70 (MQ=255)
                                                                cAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCCACAAACg       >  1:2696998/1‑70 (MQ=255)
                                                                  aCTAACATTAACGCCCAGCCCGCGAGGAAGCTCAcgccg                                    >  1:3456425/1‑39 (MQ=255)
                                                                  aCTAACATTAACGCCCAGCCCGCGAGGAAGCTCAcgccg                                    >  1:2877926/1‑39 (MQ=255)
                                                                  aCTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCCACAAACg       >  1:7454303/1‑68 (MQ=255)
                                                                  aCTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCCACAAACGCa     <  1:4618683/70‑1 (MQ=255)
                                                                   cTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCCACAAACg       >  1:6913039/1‑67 (MQ=255)
                                                                    tAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAAc                               <  1:4014599/42‑1 (MQ=255)
                                                                    tAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAAc                               <  1:7201484/42‑1 (MQ=255)
                                                                    tAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACg                 >  1:7452327/1‑56 (MQ=255)
                                                                    tAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCCACAAACGCAGAt  >  1:6044706/1‑71 (MQ=255)
                                                                    |                                                                      
CCGGACGGCACAGCAATACAACCTTATTCATGACTTACGCCCTTGCGCTTTAAACGGATAGCACCAACTAACATTAACGCCCAGCCCGCGAGGAAGCTCACGCCGCCAACCGGAGTGACAAACGCCCACAAACGCAGAT  >  W3110S.gb/2939869‑2940007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: