| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,041,067 | C→A | 9.0% | intergenic (+118/+78) | ygfZ → / ← yqfA | predicted folate‑dependent regulatory protein/predicted oxidoreductase, inner membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,041,067 | 0 | C | A | 9.0% | 204.8 / 4.8 | 66 | intergenic (+118/+78) | ygfZ/yqfA | predicted folate‑dependent regulatory protein/predicted oxidoreductase, inner membrane subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/36); new base A (2/4); total (26/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01 | |||||||||||
ATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAA > W3110S.gb/3041002‑3041133 | aTCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCAc < 1:1399987/68‑1 (MQ=255)aTCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCAc < 1:997683/68‑1 (MQ=255) cATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCa > 1:1507099/1‑65 (MQ=255) aTGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGaa < 1:2396033/71‑1 (MQ=255) tGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAAt < 1:6561896/71‑1 (MQ=255) tGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAAt < 1:7439787/71‑1 (MQ=255) tGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATcc < 1:6353216/69‑1 (MQ=255) tGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCgg > 1:2600312/1‑71 (MQ=255) aTTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATcc < 1:6018907/65‑1 (MQ=255) aTTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGt < 1:5419677/71‑1 (MQ=255) tttGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGtt < 1:661138/71‑1 (MQ=255) tttGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGtt > 1:6758347/1‑71 (MQ=255) tGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGcc > 1:5625290/1‑56 (MQ=37) aTGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGc < 1:6216130/71‑1 (MQ=255) tGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCt > 1:7198019/1‑71 (MQ=255) tGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCt < 1:6972597/71‑1 (MQ=255) tGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGAGTTCACGCCGAATCCGGCAGTtgtg > 1:4866994/1‑69 (MQ=255) ccTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCa > 1:643948/1‑47 (MQ=255) cTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCa > 1:2513226/1‑60 (MQ=255) cTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCa > 1:7731168/1‑60 (MQ=255) cTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCa > 1:653681/1‑60 (MQ=255) tATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATg > 1:1539361/1‑71 (MQ=255) tATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTAACGCCGAATCCGGCAGTTGTGCTCCGATg > 1:1494849/1‑71 (MQ=255) acaacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTg < 1:6239846/71‑1 (MQ=255) acaacaGGGACGAGGCCCGTAGATCAGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCt < 1:7365896/70‑1 (MQ=255) caacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGcc < 1:5229617/68‑1 (MQ=255) caacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGcc < 1:5909706/68‑1 (MQ=255) caacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGcc < 1:2517497/68‑1 (MQ=255) caacaGGGACGAGGACCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGa > 1:4316130/1‑64 (MQ=255) aacaGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGcct < 1:1932087/42‑2 (MQ=18) aacaGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGcct < 1:1873169/42‑2 (MQ=18) aacaGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGcct < 1:1859930/42‑2 (MQ=18) aacaGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGcct < 1:5838786/42‑2 (MQ=18) aacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGaa < 1:2839773/44‑1 (MQ=255) caGGGACGAGGCCCGTAGATCGGATAAGGCGTTCa > 1:6849127/1‑35 (MQ=255) caGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAAt < 1:6754047/43‑1 (MQ=255) caGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAAt < 1:3116404/43‑1 (MQ=255) caGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAAt < 1:491414/43‑1 (MQ=255) caGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGc > 1:5286024/1‑71 (MQ=255) aGGGACGAGGCCCGTAGATCGGATAAGGCGTTCAc < 1:5069377/35‑1 (MQ=255) gggACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGtt > 1:4486976/1‑50 (MQ=255) gACGAGTCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACg > 1:1014840/1‑71 (MQ=255) cGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCt > 1:6197303/1‑71 (MQ=255) gCCCGTCGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc < 1:7732961/70‑1 (MQ=255) gCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTcc < 1:7079246/49‑1 (MQ=255) ccGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCt < 1:4223146/65‑1 (MQ=255) ccGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCt < 1:2614173/65‑1 (MQ=255) ccGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGcgcg < 1:6229392/71‑1 (MQ=255) ccGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGcgcg > 1:6686788/1‑71 (MQ=255) gTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc > 1:2554602/1‑71 (MQ=255) gTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGAGCGTc > 1:2493260/1‑71 (MQ=255) tAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc > 1:2753443/1‑65 (MQ=255) gATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCtt < 1:2160858/70‑1 (MQ=255) gATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTa > 1:3436937/1‑71 (MQ=255) gATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTa > 1:3445948/1‑71 (MQ=255) ggATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAg < 1:5409450/71‑1 (MQ=255) ggATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAg < 1:4108870/71‑1 (MQ=255) aTAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGc < 1:5090092/71‑1 (MQ=255) ggCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgcgc < 1:2445158/55‑1 (MQ=255) gCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTgg > 1:3686811/1‑51 (MQ=255) gCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAc > 1:3731967/1‑70 (MQ=255) gCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa < 1:353770/71‑1 (MQ=255) cGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaa < 1:7340001/71‑1 (MQ=255) cGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaa < 1:5180625/71‑1 (MQ=255) ttCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTAt < 1:161036/58‑1 (MQ=255) tCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTAt < 1:7729320/57‑1 (MQ=255) | ATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAA > W3110S.gb/3041002‑3041133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |