| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,065,979 | A→T | 6.1% | intergenic (‑150/+17) | ygfI ← / ← yggE | predicted DNA‑binding transcriptional regulator/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,065,979 | 0 | A | T | 6.1% | 181.1 / 3.7 | 65 | intergenic (‑150/+17) | ygfI/yggE | predicted DNA‑binding transcriptional regulator/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (35/26); new base T (2/2); total (37/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
TCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGG > W3110S.gb/3065913‑3066047 | tCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTc < 1:1677033/71‑1 (MQ=255) ggTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTc > 1:825624/1‑67 (MQ=255) ttttACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGt < 1:450925/64‑1 (MQ=255) ttttACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTa < 1:7843969/71‑1 (MQ=255) acagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACaa < 1:6990938/70‑1 (MQ=255) aaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAatat > 1:7698886/1‑70 (MQ=255) aaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAtatta > 1:156363/1‑71 (MQ=255) ttacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtg < 1:7009463/71‑1 (MQ=255) tacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtgt > 1:6126835/1‑71 (MQ=255) aaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgctg > 1:2637/1‑69 (MQ=255) aaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCa < 1:3675236/71‑1 (MQ=255) aaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAg > 1:5978788/1‑71 (MQ=255) aggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGtt < 1:1879033/71‑1 (MQ=255) gCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCggg > 1:1560559/1‑41 (MQ=25) gCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTc < 1:787196/38‑1 (MQ=255) aaTATCGGGTAAAGGCATTAGCCCGACGAATACGTCgg > 1:2887482/1‑38 (MQ=255) aaTATCGGGTAAAGGCATTAGCCCGACGAATACGTCgg > 1:1052574/1‑38 (MQ=255) atatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGtttt < 1:7057984/68‑1 (MQ=255) atatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGc < 1:512349/71‑1 (MQ=255) atatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGc > 1:4442299/1‑71 (MQ=255) tatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg > 1:6797741/1‑71 (MQ=255) atCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgc < 1:1671475/54‑1 (MQ=255) atCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCgg > 1:5195344/1‑71 (MQ=255) cGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCggg > 1:7243138/1‑70 (MQ=255) gggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgtt < 1:3890399/71‑1 (MQ=255) ggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg < 1:5532412/66‑1 (MQ=255) ggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgtt < 1:7095456/70‑1 (MQ=255) aGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGa > 1:3736102/1‑70 (MQ=255) aGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGAt > 1:6921970/1‑71 (MQ=255) gCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgt < 1:2932787/50‑1 (MQ=255) gCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc < 1:2162935/71‑1 (MQ=255) gCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATc > 1:7622053/1‑70 (MQ=255) aTTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtgt < 1:1914890/50‑1 (MQ=255) ttAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg < 1:1040417/48‑1 (MQ=255) ttAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAg < 1:4912364/54‑1 (MQ=255) tAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAg > 1:5083827/1‑71 (MQ=255) aGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCgg > 1:6378654/1‑55 (MQ=255) gCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAgg < 1:3881366/70‑1 (MQ=255) cccGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcac < 1:6333582/66‑1 (MQ=255) ccGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGa < 1:2794715/60‑1 (MQ=39) cgacgtATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg > 1:306314/1‑50 (MQ=38) acgaATACGTCGGGCTACAAATATTATTGTGCTGCAGgt > 1:630953/1‑39 (MQ=255) acgaATACGTCGGGCTACAAATATTATTGTGCTGCAGgt > 1:3934027/1‑39 (MQ=255) acgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGtttt > 1:4081943/1‑44 (MQ=255) acgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTaa > 1:1195218/1‑71 (MQ=255) acgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTaa > 1:796768/1‑71 (MQ=255) acgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTaa > 1:4834639/1‑71 (MQ=255) acgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTaa > 1:3664949/1‑71 (MQ=255) cgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAg > 1:1970609/1‑45 (MQ=255) cgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAg > 1:4711786/1‑45 (MQ=255) cgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAg > 1:1961341/1‑45 (MQ=255) cgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcac < 1:98553/61‑1 (MQ=255) cgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcac < 1:1448706/61‑1 (MQ=255) cgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcac < 1:6135580/61‑1 (MQ=255) cgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAAc > 1:7191213/1‑71 (MQ=255) gaATACGTCGGTCTACAAATATTATTGTGCTGCAGGTGtt < 1:6935673/40‑1 (MQ=255) gaATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg > 1:4330746/1‑38 (MQ=255) gaATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg > 1:2589931/1‑38 (MQ=255) gaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCgg > 1:7717649/1‑47 (MQ=255) aTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTa > 1:3887123/1‑41 (MQ=255) aTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTa > 1:5072666/1‑41 (MQ=255) tACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttgt < 1:1919862/49‑1 (MQ=255) tACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGtt < 1:1418581/62‑1 (MQ=255) tACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTgg > 1:3790581/1‑70 (MQ=255) tACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGAGGGTTGTTGATCCACAGGTTCTAACTgg > 1:2313190/1‑70 (MQ=255) | TCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGG > W3110S.gb/3065913‑3066047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |