| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,066,112 | C→T | 5.1% | V209M (GTG→ATG) | yggE ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,066,112 | 0 | C | T | 5.1% | 257.7 / 2.5 | 78 | V209M (GTG→ATG) | yggE | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (46/28); new base T (2/2); total (48/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
AACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGAAACATGGTAGCGCAC > W3110S.gb/3066042‑3066181 | aaCTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGacac > 1:5949065/1‑71 (MQ=255) aCTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACAcc > 1:5447762/1‑71 (MQ=255) tGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCgg > 1:2167098/1‑71 (MQ=255) gAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcg > 1:4483357/1‑71 (MQ=255) ccACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCa < 1:138852/71‑1 (MQ=255) caTCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGAGACCGGCGCGGCATCg > 1:2684668/1‑71 (MQ=255) caTCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCg > 1:7354735/1‑71 (MQ=255) caTCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCg > 1:3708566/1‑71 (MQ=255) aTCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCgg > 1:1729876/1‑71 (MQ=255) tCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATc > 1:7167843/1‑68 (MQ=255) tCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGc < 1:536930/71‑1 (MQ=255) cGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCt > 1:1874731/1‑71 (MQ=255) cGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCt > 1:452080/1‑71 (MQ=255) tGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCttt < 1:3033223/68‑1 (MQ=255) tGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTtc < 1:7320798/69‑1 (MQ=255) gATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCTGACACCGGCGCGGCATCGGCTTtc < 1:6039943/68‑1 (MQ=255) atcatcAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCttt > 1:5397582/1‑66 (MQ=255) atcatcAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTtcatc < 1:4046131/70‑1 (MQ=255) tcatcaAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTcatcat > 1:6514024/1‑71 (MQ=255) catcaAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTtcatca < 1:6848660/69‑1 (MQ=255) catcaAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCatcatc > 1:3576331/1‑71 (MQ=255) atcaAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACATCGGCGCGGCATCGGCt < 1:2450602/61‑1 (MQ=255) atcaAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACATCGGCGCGGCATCGGCt < 1:1895903/61‑1 (MQ=255) atcaAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATcc < 1:310311/71‑1 (MQ=255) tcaAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGc < 1:4595001/59‑1 (MQ=255) caAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGacac > 1:6894521/1‑43 (MQ=255) caAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACATCGGCGCGGCATCGGCttt > 1:5889973/1‑61 (MQ=255) caAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACATCGGCGCGGCATCGGCttt > 1:4082449/1‑61 (MQ=255) aaCTGAATAGCGGCCTGCTCGTATGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCAc < 1:4757592/71‑1 (MQ=255) aaCTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCAt < 1:6918812/52‑1 (MQ=255) aaCTGAATAGCGGCCTGCTCGTAAGTTTCATGGGCGGACACCGGCGCGGCAt < 1:1804362/52‑1 (MQ=255) tGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCAt > 1:2355072/1‑71 (MQ=255) gAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCAtt > 1:3288532/1‑71 (MQ=255) gAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCAtt > 1:4328047/1‑71 (MQ=255) aTAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATtgg > 1:7683322/1‑71 (MQ=255) tAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATtggg < 1:5767750/71‑1 (MQ=255) aGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATtgggc > 1:242075/1‑71 (MQ=255) aGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATtgggc > 1:4367993/1‑71 (MQ=255) gCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCttt > 1:5875236/1‑50 (MQ=255) gCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCttt > 1:3231404/1‑50 (MQ=255) gCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATtgggct > 1:1427028/1‑71 (MQ=255) cGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATtgggctg < 1:37517/71‑1 (MQ=255) gCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATtgggctgg < 1:2692694/70‑1 (MQ=255) ccTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATtgggctggg > 1:889246/1‑70 (MQ=255) gCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCgg < 1:3628511/39‑1 (MQ=255) cTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATc > 1:5295292/1‑36 (MQ=255) cTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCgg < 1:4953917/38‑1 (MQ=255) cTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGa > 1:6636344/1‑70 (MQ=255) gTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATtg > 1:2637943/1‑56 (MQ=255) gTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGt > 1:3331620/1‑71 (MQ=255) tAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATcc > 1:3212297/1‑46 (MQ=255) tAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCAt > 1:5023387/1‑53 (MQ=255) tAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGt > 1:3488566/1‑70 (MQ=255) tAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGt < 1:531436/70‑1 (MQ=255) aaGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTg > 1:7644070/1‑71 (MQ=255) aaGTTTCCTGGGCGGACACCGCAGCGGCATCGGCTTTCATCATCCGc > 1:3453240/1‑47 (MQ=39) aaGTTTCCTGG‑‑GGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGtt > 1:329318/1‑68 (MQ=255) aGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTtca > 1:5768664/1‑38 (MQ=255) aGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTtc > 1:3931305/1‑37 (MQ=255) aGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTg > 1:1434307/1‑70 (MQ=255) aGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTg > 1:67108/1‑70 (MQ=255) gTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGa < 1:6377449/71‑1 (MQ=255) tttCCTGGGCGGACACCGGTGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATa < 1:6445514/64‑1 (MQ=255) tttCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATa < 1:3823751/64‑1 (MQ=255) ttCCTGGGCGGACACCGGCGCGGCATCGGCTTTcatcat > 1:7397183/1‑39 (MQ=255) ttCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGaaa > 1:7463104/1‑71 (MQ=255) ttCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGa < 1:484046/69‑1 (MQ=255) tCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGaaa > 1:948689/1‑70 (MQ=255) tCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGaaa > 1:2404069/1‑70 (MQ=255) tCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGaaa > 1:2021206/1‑70 (MQ=255) ccTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGAAAc < 1:7762477/70‑1 (MQ=255) cTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATcc > 1:806671/1‑38 (MQ=255) cTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATcc > 1:108098/1‑38 (MQ=255) cTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGAAAc < 1:294461/69‑1 (MQ=255) gggCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCAATGGGCTGGGCTGATAGTTgg > 1:7148665/1‑63 (MQ=255) ggCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGAAACATgg < 1:3619193/70‑1 (MQ=255) gCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGt < 1:6117803/58‑1 (MQ=255) gCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGt < 1:4573813/58‑1 (MQ=255) gCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTgg > 1:3573719/1‑61 (MQ=255) ggACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGAAACATg < 1:2083924/66‑1 (MQ=255) gACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGAAACATGGTAgcg > 1:1223150/1‑71 (MQ=255) acCGGCGCGGCATCGGCTTTCATCATCCGCACCATtgggctgggc < 1:948562/45‑1 (MQ=255) acCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATa < 1:268568/50‑1 (MQ=255) acCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGAAACATGGTAGCGCAc > 1:3791740/1‑71 (MQ=255) | AACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCTTTCATCATCCGCACCATTGGGCTGGGCTGATAGTTGGAAACATGGTAGCGCAC > W3110S.gb/3066042‑3066181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |