| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,127,723 | G→T | 5.2% | Y383* (TAC→TAA) | yghO ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,127,723 | 0 | G | T | 5.2% | 374.6 / 3.3 | 114 | Y383* (TAC→TAA) | yghO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (57/51); new base T (4/2); total (61/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
ATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGAT > W3110S.gb/3127654‑3127791 | aTTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCgg > 1:786499/1‑70 (MQ=255)aTTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt < 1:5101136/71‑1 (MQ=255)aTTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt < 1:5225316/71‑1 (MQ=255)aTTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt < 1:1888859/71‑1 (MQ=255)aTTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt < 1:6929190/71‑1 (MQ=255) cGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCgg > 1:7938908/1‑67 (MQ=255) cTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc > 1:7310257/1‑71 (MQ=255) tCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCt > 1:6530646/1‑71 (MQ=255) gcggTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGt < 1:4451706/71‑1 (MQ=255) tGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACgg > 1:2249890/1‑71 (MQ=255) gCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGc > 1:138554/1‑71 (MQ=255) ccGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTaa < 1:7274116/58‑1 (MQ=255) ccGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTaa < 1:344141/58‑1 (MQ=255) cGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc < 1:7200442/60‑1 (MQ=255) cTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCg > 1:6638683/1‑71 (MQ=255) gagaTGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGccc > 1:2395606/1‑71 (MQ=255) gagaTGAACCTGAGATAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGc < 1:2719397/69‑1 (MQ=255) agaTGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcccc < 1:1621728/71‑1 (MQ=255) gaTGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt < 1:3660572/48‑1 (MQ=255) gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTa < 1:5976551/46‑1 (MQ=255) gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACgg < 1:83994/58‑1 (MQ=255) gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACgg < 1:2565791/58‑1 (MQ=255) gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa > 1:4720803/1‑69 (MQ=255) gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa > 1:1155967/1‑69 (MQ=255) gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATg > 1:6242560/1‑71 (MQ=255) aaCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcccc < 1:6228381/66‑1 (MQ=255) aaCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATg < 1:2115059/70‑1 (MQ=255) aCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAAc > 1:3115904/1‑46 (MQ=255) aCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTAc < 1:4866484/54‑1 (MQ=255) aCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTcc > 1:2172797/1‑60 (MQ=255) aCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCg > 1:4929465/1‑71 (MQ=255) ccTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCgg > 1:5900339/1‑41 (MQ=255) tGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTc > 1:2156115/1‑71 (MQ=255) gAGTTAGCTGGTATTAAATCTGCTTTTCGTACAAGCGTTACCGATTGt > 1:6367841/1‑48 (MQ=18) gAGTTAGCTGGTATTAAATCTGCTTTTCGTACAAGCGTTACCGATTGt > 1:4547950/1‑48 (MQ=18) gAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTc > 1:2193449/1‑70 (MQ=255) aGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTc < 1:5369322/69‑1 (MQ=255) ttAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGc < 1:2872702/71‑1 (MQ=255) ttAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGc < 1:4911416/71‑1 (MQ=255) ttAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGc < 1:3282429/71‑1 (MQ=255) tAGCTGGTTTTAAATCTGCTTTTCATACAATCGGTAACGCTTGTa < 1:4821999/45‑1 (MQ=255) aGCTGGTATTAAATCTGCTTTTCATTCAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCa < 1:1234593/70‑1 (MQ=255) aGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCAt > 1:198396/1‑71 (MQ=255) aGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCAt > 1:1599782/1‑71 (MQ=255) aGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCAt > 1:675269/1‑71 (MQ=255) ggTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa > 1:5589114/1‑71 (MQ=255) gTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc < 1:2224459/49‑1 (MQ=255) gTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCa > 1:7356409/1‑65 (MQ=255) gTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATgaat > 1:2129873/1‑67 (MQ=255) gTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa < 1:6970731/70‑1 (MQ=255) gTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa < 1:4821321/70‑1 (MQ=255) gTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt < 1:3726235/71‑1 (MQ=255) gTAATAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATgaat > 1:2485088/1‑67 (MQ=255) aTTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt > 1:569929/1‑70 (MQ=255) aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc > 1:7836909/1‑44 (MQ=255) aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc > 1:3191050/1‑44 (MQ=255) aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt > 1:2090567/1‑66 (MQ=255) aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg > 1:2588525/1‑71 (MQ=255) aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg > 1:7057650/1‑71 (MQ=255) aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg > 1:1944091/1‑71 (MQ=255) aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg > 1:7277005/1‑71 (MQ=255) aaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGtt > 1:4423325/1‑63 (MQ=255) aTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGtt < 1:3158308/52‑1 (MQ=255) aTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCa < 1:4001639/58‑1 (MQ=255) aTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt < 1:3090043/68‑1 (MQ=255) aTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt < 1:1409335/68‑1 (MQ=255) cTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGc > 1:6623264/1‑68 (MQ=255) cTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTg > 1:3902834/1‑71 (MQ=255) cTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTg > 1:5332625/1‑71 (MQ=255) tGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa > 1:6347583/1‑43 (MQ=255) tGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATga > 1:3809375/1‑66 (MQ=255) gCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa < 1:7311117/59‑1 (MQ=255) gCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa < 1:2815859/59‑1 (MQ=255) gCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa > 1:2561745/1‑71 (MQ=255) cTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa > 1:4886344/1‑58 (MQ=255) cTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGt < 1:7083662/69‑1 (MQ=255) ttttCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGc > 1:1605134/1‑64 (MQ=255) ttttCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGc > 1:3712389/1‑64 (MQ=255) ttCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAtt < 1:1733721/69‑1 (MQ=255) tCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGt > 1:6287222/1‑71 (MQ=255) cATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGt < 1:5706239/70‑1 (MQ=255) gtACAAGCGTTACCGATTGTACGGCTCCGCCCCAATGCGTTCCAg < 1:3854189/44‑1 (MQ=18) gtACAAGCGTTACCGATTGTACGGCTCCGCCCCAATGCGTTCCAg < 1:168167/44‑1 (MQ=18) aTACAATCGGTAACGCTTGTACGGCTCCGCCCCaa > 1:4790179/1‑35 (MQ=255) aTACAATCGGTAACGCTTGTACGGCTCCGCCCCaa > 1:6996176/1‑35 (MQ=255) tACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc < 1:4442199/71‑1 (MQ=255) tACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc < 1:1206496/71‑1 (MQ=255) aCAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc < 1:4039659/70‑1 (MQ=255) aCAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc > 1:2618822/1‑70 (MQ=255) aCAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc < 1:2850708/70‑1 (MQ=255) aCAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc > 1:3178539/1‑70 (MQ=255) aCAAGCGTTACCGATTGTACGGCTCCGCCCCAATGCGTTCCAGCa > 1:193053/1‑45 (MQ=21) aCAAGCGTTACCGATTGTACGGCTCCGCCCCAATGCGTTCCAGCa > 1:3055404/1‑45 (MQ=21) cAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc < 1:4915188/69‑1 (MQ=255) cAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa < 1:2376114/71‑1 (MQ=255) cAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa < 1:1778661/71‑1 (MQ=255) aaTCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAg > 1:2683728/1‑71 (MQ=255) aaTCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAg > 1:2206349/1‑71 (MQ=255) ggTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGAt > 1:107735/1‑70 (MQ=255) gTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt < 1:6235845/45‑1 (MQ=255) | ATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGAT > W3110S.gb/3127654‑3127791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |