Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,127,729 C→A 5.1% K381N (AAG→AAT yghO ← predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,127,7290CA 5.1% 384.3 / 2.9 115K381N (AAG→AATyghOpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (59/50);  new base A (4/2);  total (63/52)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 121 total aligned reads displayed.

CTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGAC  >  W3110S.gb/3127659‑3127798
                                                                      |                                                                     
cTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc                                                                       >  1:7310257/1‑71 (MQ=255)
 tCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCt                                                                      >  1:6530646/1‑71 (MQ=255)
       gTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTg                                                                    <  1:2205063/67‑1 (MQ=255)
        tGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACgg                                                               >  1:2249890/1‑71 (MQ=255)
           cGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc                                                                       <  1:7200442/60‑1 (MQ=255)
             cTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCg                                                          >  1:6638683/1‑71 (MQ=255)
                gagaTGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGccc                                                       >  1:2395606/1‑71 (MQ=255)
                gagaTGAACCTGAGATAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGc                                                         <  1:2719397/69‑1 (MQ=255)
                 agaTGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcccc                                                      <  1:1621728/71‑1 (MQ=255)
                  gaTGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcccc                                                      >  1:5927556/1‑70 (MQ=255)
                   aTGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcccc                                                      <  1:6846173/69‑1 (MQ=255)
                     gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACgg                                                               <  1:83994/58‑1 (MQ=255)
                     gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACgg                                                               <  1:2565791/58‑1 (MQ=255)
                     gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACgg                                                               <  1:4773566/58‑1 (MQ=255)
                     gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa                                                    >  1:4720803/1‑69 (MQ=255)
                     gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa                                                    >  1:1155967/1‑69 (MQ=255)
                     gAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATg                                                  >  1:6242560/1‑71 (MQ=255)
                      aaCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATg                                                  <  1:2115059/70‑1 (MQ=255)
                       aCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTAc                                                                 <  1:4866484/54‑1 (MQ=255)
                       aCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACg                                                                <  1:7320164/55‑1 (MQ=255)
                       aCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTcc                                                           >  1:2172797/1‑60 (MQ=255)
                       aCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCg                                                >  1:4929465/1‑71 (MQ=255)
                           gAGTTAGCTGGTATTAAATCTGCTTTTCGTACAAGCGTTACCGATTGt                                                                   >  1:4547950/1‑48 (MQ=18)
                           gAGTTAGCTGGTATTAAATCTGCTTTTCGTACAAGCGTTACCGATTGt                                                                   >  1:6367841/1‑48 (MQ=18)
                           gAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa                                                    >  1:3789652/1‑63 (MQ=255)
                           gAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTc                                             >  1:2193449/1‑70 (MQ=255)
                            aGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTg                                                                    >  1:2632657/1‑46 (MQ=255)
                            aGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTc                                             <  1:5369322/69‑1 (MQ=255)
                              ttAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGc                                         <  1:3282429/71‑1 (MQ=255)
                              ttAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGc                                         <  1:4911416/71‑1 (MQ=255)
                              ttAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGc                                         <  1:2872702/71‑1 (MQ=255)
                               tAGCTGGTTTTAAATCTGCTTTTCATACAATCGGTAACGCTTGTa                                                                  <  1:4821999/45‑1 (MQ=255)
                                aGCTGGTATTAAATCTGCTTTTCATTCAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCa                                        <  1:1234593/70‑1 (MQ=255)
                                aGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCAt                                       >  1:198396/1‑71 (MQ=255)
                                aGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCAt                                       >  1:1599782/1‑71 (MQ=255)
                                   tGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa                                                    >  1:3268935/1‑55 (MQ=255)
                                    ggTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa                                   >  1:5589114/1‑71 (MQ=255)
                                     gTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATgaat                                   >  1:2129873/1‑67 (MQ=255)
                                     gTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa                                   <  1:4821321/70‑1 (MQ=255)
                                     gTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa                                   <  1:6970731/70‑1 (MQ=255)
                                      tATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt                                  >  1:1763978/1‑70 (MQ=255)
                                       aTTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                 >  1:569929/1‑70 (MQ=255)
                                          aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                        >  1:7836909/1‑44 (MQ=255)
                                          aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt                                  >  1:2090567/1‑66 (MQ=255)
                                          aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg                             >  1:7057650/1‑71 (MQ=255)
                                          aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg                             >  1:2588525/1‑71 (MQ=255)
                                          aaaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg                             >  1:7277005/1‑71 (MQ=255)
                                           aaTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGtt                                    >  1:4423325/1‑63 (MQ=255)
                                            aTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGtt                                              <  1:3158308/52‑1 (MQ=255)
                                            aTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCa                                        <  1:4001639/58‑1 (MQ=255)
                                            aTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt                              <  1:3090043/68‑1 (MQ=255)
                                            aTCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt                              <  1:1409335/68‑1 (MQ=255)
                                              cTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGc                            >  1:6623264/1‑68 (MQ=255)
                                              cTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTg                         >  1:3902834/1‑71 (MQ=255)
                                              cTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTg                         >  1:5332625/1‑71 (MQ=255)
                                               tGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATga                            >  1:3809375/1‑66 (MQ=255)
                                                gCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa                                   <  1:7311117/59‑1 (MQ=255)
                                                gCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa                                   <  1:2815859/59‑1 (MQ=255)
                                                gCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                       >  1:2561745/1‑71 (MQ=255)
                                                gCTTTTCATACAATCGGTAACGCTTCTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                       >  1:2479796/1‑71 (MQ=255)
                                                 cTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa                                   >  1:4886344/1‑58 (MQ=255)
                                                 cTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGt                        <  1:7083662/69‑1 (MQ=255)
                                                  ttttCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGc                            >  1:3712389/1‑64 (MQ=255)
                                                  ttttCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGc                            >  1:1605134/1‑64 (MQ=255)
                                                    ttCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAtt                     <  1:1733721/69‑1 (MQ=255)
                                                     tCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGt                  >  1:6287222/1‑71 (MQ=255)
                                                      cATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGt                  <  1:5706239/70‑1 (MQ=255)
                                                       gtACAAGCGTTACCGATTGTACGGCTCCGCCCCAATGCGTTCCAg                                          <  1:3854189/44‑1 (MQ=18)
                                                       gtACAAGCGTTACCGATTGTACGGCTCCGCCCCAATGCGTTCCAg                                          <  1:168167/44‑1 (MQ=18)
                                                       aTACAATCGGTAACGCTTGTACGGCTCCGCCCCaa                                                    >  1:6996176/1‑35 (MQ=255)
                                                        tACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc               <  1:1206496/71‑1 (MQ=255)
                                                        tACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc               <  1:4246644/71‑1 (MQ=255)
                                                        tACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc               <  1:4442199/71‑1 (MQ=255)
                                                         aCAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc               >  1:2618822/1‑70 (MQ=255)
                                                         aCAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc               <  1:2850708/70‑1 (MQ=255)
                                                         aCAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc               >  1:3178539/1‑70 (MQ=255)
                                                         aCAAGCGTTACCGATTGTACGGCTCCGCCCCAATGCGTTCCAGCa                                        >  1:193053/1‑45 (MQ=21)
                                                         aCAAGCGTTACCGATTGTACGGCTCCGCCCCAATGCGTTCCAGCa                                        >  1:3055404/1‑45 (MQ=21)
                                                          cAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc               <  1:4915188/69‑1 (MQ=255)
                                                          cAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa             <  1:2376114/71‑1 (MQ=255)
                                                          cAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa             <  1:1778661/71‑1 (MQ=255)
                                                          cAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa             <  1:631524/71‑1 (MQ=255)
                                                           aaTCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAg            >  1:2206349/1‑71 (MQ=255)
                                                            aTCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAgg           <  1:204679/71‑1 (MQ=255)
                                                               ggTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGAt         >  1:107735/1‑70 (MQ=255)
                                                                gTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                 <  1:6235845/45‑1 (MQ=255)
                                                                 tAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCa      <  1:924937/71‑1 (MQ=255)
                                                                  aaCGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt     >  1:6365151/1‑71 (MQ=255)
                                                                  aaCGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt     >  1:5286114/1‑71 (MQ=255)
                                                                  aaCGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt     >  1:4784051/1‑71 (MQ=255)
                                                                  aaCGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt     >  1:1749604/1‑71 (MQ=255)
                                                                   aCGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt     <  1:355750/70‑1 (MQ=255)
                                                                   aCGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt     <  1:2347683/70‑1 (MQ=255)
                                                                   aCGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt     <  1:7377682/70‑1 (MQ=255)
                                                                   aCGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt     <  1:1053806/70‑1 (MQ=255)
                                                                    cGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt                                  <  1:5107373/40‑1 (MQ=255)
                                                                     gCTTGTACGGCTACGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt     <  1:2906594/68‑1 (MQ=255)
                                                                      cTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTc                                >  1:4428102/1‑40 (MQ=255)
                                                                      cTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCg              <  1:4135262/58‑1 (MQ=255)
                                                                      cTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGAc  >  1:3247558/1‑70 (MQ=255)
                                                                      |                                                                     
CTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGAC  >  W3110S.gb/3127659‑3127798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: