Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,142,816 C→G 5.6% S2T (AGT→ACT) 
E327D (GAG→GAC
hybB ←
hybA ←
predicted hydrogenase 2 cytochrome b type component
hydrogenase 2 4Fe‑4S ferredoxin‑type component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,142,8160CG 5.6% 499.3 / 12.4 160S2T (AGT→ACT) 
E327D (GAG→GAC
hybB
hybA
predicted hydrogenase 2 cytochrome b type component
hydrogenase 2 4Fe‑4S ferredoxin‑type component
Reads supporting (aligned to +/- strand):  ref base C (72/79);  new base G (3/6);  total (75/85)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 164 total aligned reads displayed.

CGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGC  >  W3110S.gb/3142748‑3142884
                                                                    |                                                                    
    tAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGAc                                                                      >  1:2225890/1‑65 (MQ=255)
    tAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGAc                                                                      >  1:4492166/1‑65 (MQ=255)
    tAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGa                                                                >  1:1604775/1‑71 (MQ=255)
    tAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGa                                                                >  1:347488/1‑71 (MQ=255)
     aaCGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGa                                                                <  1:4800388/70‑1 (MQ=255)
     aaCGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGAt                                                               >  1:3990048/1‑71 (MQ=255)
             aaaaaTCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATc                                                              <  1:7499089/64‑1 (MQ=255)
             aaaaaTCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGtctc                                                         <  1:1479459/69‑1 (MQ=255)
             aaaaaTCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTctcct                                                       <  1:6915697/71‑1 (MQ=255)
              aaaaTCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATAGTctcctc                                                      >  1:2913754/1‑71 (MQ=255)
               aaaTCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGAt                                                               >  1:3837937/1‑61 (MQ=255)
               aaaTCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGAt                                                               >  1:209969/1‑61 (MQ=255)
               aaaTCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCttctcc                                                     >  1:3532847/1‑71 (MQ=255)
               aaaTCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCtcctcc                                                     >  1:1200690/1‑71 (MQ=255)
                   cATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCcgtcg                                                 >  1:317898/1‑71 (MQ=255)
                   cATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCcgtc                                                  >  1:328507/1‑70 (MQ=255)
                     tGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGAGGATCATGACTCATGATCGTCTCCTCcgtcgt                                                <  1:6426060/70‑1 (MQ=255)
                       aCCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGAc                                                                      <  1:3318094/46‑1 (MQ=255)
                       aCCGGTTTACTGATGATTTTGCCGCCCAGCGGTCGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGa                                              <  1:389280/70‑1 (MQ=255)
                       aCCGGTGTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTc                                                                    >  1:1020690/1‑48 (MQ=255)
                        ccGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATg                                            >  1:6173947/1‑71 (MQ=255)
                         cGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCcg                                                    <  1:5119783/62‑1 (MQ=255)
                         cGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATgg                                           <  1:70689/71‑1 (MQ=255)
                          ggTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCcg                                                    <  1:5348116/61‑1 (MQ=255)
                           gTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGa                                              >  1:7587491/1‑66 (MQ=255)
                           gTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTc                                         >  1:473104/1‑71 (MQ=255)
                                cTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGtttt                                    <  1:1044018/71‑1 (MQ=255)
                                cTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGtttt                                    <  1:4886426/71‑1 (MQ=255)
                                cTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGtttt                                    <  1:4820031/71‑1 (MQ=255)
                                cTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGtttt                                    >  1:1386519/1‑71 (MQ=255)
                                cTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGttt                                     <  1:3544172/70‑1 (MQ=255)
                                 tgatgaTTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATg                                            <  1:5969832/62‑1 (MQ=255)
                                   atgatTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATgg                                           <  1:5413737/61‑1 (MQ=255)
                                   atgatTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATgg                                           <  1:5659083/61‑1 (MQ=255)
                                   atgatTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGttttt                                   <  1:1813567/69‑1 (MQ=255)
                                      atTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGttttt                                   <  1:7073498/66‑1 (MQ=255)
                                      atTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGttttt                                   <  1:4142620/66‑1 (MQ=255)
                                       ttttGCGGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGtt                             >  1:1024685/1‑71 (MQ=255)
                                       ttttGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGtt                             >  1:761029/1‑71 (MQ=255)
                                       ttttGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGtt                             <  1:3521574/71‑1 (MQ=255)
                                        tttGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTg                            >  1:4287841/1‑71 (MQ=255)
                                         ttGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGc                           >  1:6038524/1‑71 (MQ=255)
                                         ttGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGc                           >  1:3838572/1‑71 (MQ=255)
                                         ttGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGc                           >  1:6272474/1‑71 (MQ=255)
                                         ttGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGGGATGGTCGGTTTTGGTGTTGc                           >  1:2426247/1‑71 (MQ=255)
                                          tGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGc                           <  1:6487569/70‑1 (MQ=255)
                                          tGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGc                           >  1:584394/1‑70 (MQ=255)
                                          tGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGc                           <  1:7801203/70‑1 (MQ=255)
                                          tGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGc                           <  1:2985320/70‑1 (MQ=255)
                                           gccgccCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCcg                                                    <  1:694540/44‑1 (MQ=255)
                                            ccgccCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGcga                         <  1:426341/70‑1 (MQ=255)
                                              gccCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGcgacga                      <  1:157745/71‑1 (MQ=255)
                                               cccAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGcgac                        >  1:4354846/1‑68 (MQ=255)
                                               cccAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGaa                     >  1:2509158/1‑71 (MQ=255)
                                               cccAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGaa                     >  1:3728009/1‑71 (MQ=255)
                                                ccAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGttttt                                   <  1:6022979/56‑1 (MQ=255)
                                                ccAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGttttt                                   <  1:7220631/56‑1 (MQ=255)
                                                ccAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAAc                    <  1:5669177/71‑1 (MQ=255)
                                                  aGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGttt                                     <  1:3989712/52‑1 (MQ=255)
                                                  aGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTgg                                 >  1:5293951/1‑56 (MQ=255)
                                                   gCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGcga                         >  1:6879054/1‑63 (MQ=255)
                                                     ggTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGcga                         >  1:2110988/1‑61 (MQ=255)
                                                     ggTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCa               <  1:6828515/71‑1 (MQ=255)
                                                        tgtgGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGttttt                                   <  1:4636133/48‑1 (MQ=255)
                                                        tgtgGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTg                                  >  1:172792/1‑49 (MQ=255)
                                                        tgtgGATCATGACTCATGATCGTCTACTCCGTCGTGATGGTCGTTTTTgg                                 <  1:2748909/50‑1 (MQ=255)
                                                         gtgGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGc                >  1:496588/1‑66 (MQ=255)
                                                         gtgGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACg             >  1:6467819/1‑69 (MQ=255)
                                                         gtgGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGt           >  1:7365425/1‑71 (MQ=255)
                                                          tgGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGgtgt                              <  1:6114201/51‑1 (MQ=255)
                                                          tgGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGgtgt                              <  1:1163179/51‑1 (MQ=255)
                                                          tgGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGgtg                               >  1:1300645/1‑50 (MQ=255)
                                                           ggATCATGACTCATGATCATCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAg                 <  1:6138599/63‑1 (MQ=255)
                                                            gATCATGAGTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGgtgt                              <  1:6903901/49‑1 (MQ=255)
                                                            gATCATGAGTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGgtgt                              <  1:4618461/49‑1 (MQ=255)
                                                            gATCATGAGTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGgtgt                              <  1:1463880/49‑1 (MQ=255)
                                                            gATCATGAGTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGtt          >  1:7589827/1‑69 (MQ=255)
                                                            gATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGcgac                        <  1:213452/55‑1 (MQ=255)
                                                            gATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGcgac                        <  1:7817023/55‑1 (MQ=255)
                                                            gATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGcgac                        <  1:4596445/55‑1 (MQ=255)
                                                            gATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGtt          >  1:378140/1‑69 (MQ=255)
                                                            gATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTaa        <  1:1743457/71‑1 (MQ=255)
                                                            gATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTa         <  1:6302712/70‑1 (MQ=255)
                                                             aTCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGtttt                                    <  1:86186/42‑1 (MQ=255)
                                                             aTCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGtttt                                    <  1:3379146/42‑1 (MQ=255)
                                                             aTCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGtttt                                    <  1:1084695/42‑1 (MQ=255)
                                                             aTCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGtttt                                    <  1:6491405/42‑1 (MQ=255)
                                                              tCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGttttt                                   <  1:6814086/42‑1 (MQ=255)
                                                              tCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGc      <  1:67043/71‑1 (MQ=255)
                                                               cATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTg                                  >  1:5069588/1‑42 (MQ=255)
                                                               cATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGtt                             >  1:2671452/1‑47 (MQ=255)
                                                                aTGACTCATGATCGTCTCCTCCGTCTTGATGGTCGTTTTTg                                  >  1:4882145/1‑41 (MQ=255)
                                                                aTGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGtt          <  1:646900/65‑1 (MQ=255)
                                                                 tGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAAcc                   <  1:7175842/55‑1 (MQ=255)
                                                                 tGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCg   >  1:2679680/1‑71 (MQ=255)
                                                                  gACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGcg                          <  1:160377/47‑1 (MQ=255)
                                                                  gACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAg       <  1:7427852/66‑1 (MQ=255)
                                                                  gACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGc      >  1:7550768/1‑67 (MQ=255)
                                                                  gACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGc      >  1:4220592/1‑67 (MQ=255)
                                                                   aCTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGcga                         >  1:3375016/1‑47 (MQ=255)
                                                                    |                                                                    
CGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGC  >  W3110S.gb/3142748‑3142884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: