Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,186,231 A→T 5.4% N59I (AAT→ATT)  yqiG → predicted outer membrane usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,186,2310AT 5.4% 261.3 / 5.8 92N59I (AAT→ATT) yqiGpredicted outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base A (44/43);  new base T (3/2);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 108 total aligned reads displayed.

TAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGT  >  W3110S.gb/3186163‑3186300
                                                                    |                                                                     
tAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaa                                                                       >  1:2379668/1‑69 (MQ=255)
 aaCCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataa                                                                    >  1:3199240/1‑71 (MQ=255)
   ccTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataata                                                                  <  1:7711220/71‑1 (MQ=255)
     tttCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataat                                                                   >  1:7933253/1‑68 (MQ=255)
     tttCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataa                                                                    >  1:1315294/1‑67 (MQ=255)
         ccAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAgg                                                             <  1:1046096/70‑1 (MQ=255)
             tttGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataata                                                                  <  1:4116359/61‑1 (MQ=255)
             tttGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGcc                                                        <  1:2143125/71‑1 (MQ=255)
                gAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaat                                                                      >  1:2685523/1‑54 (MQ=255)
                gAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAAt                                                     >  1:4705210/1‑71 (MQ=255)
                 aaaCTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataat                                                                   <  1:4157522/56‑1 (MQ=255)
                 aaaCTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataat                                                                   <  1:3443754/56‑1 (MQ=255)
                 aaaCTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataat                                                                   <  1:7067891/56‑1 (MQ=255)
                 aaaCTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATc                                                    >  1:5416388/1‑71 (MQ=255)
                 aaaCTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATc                                                    <  1:5825408/71‑1 (MQ=255)
                  aaCTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATcc                                                   <  1:2546832/71‑1 (MQ=255)
                    cTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCAc                                                 >  1:2712744/1‑71 (MQ=255)
                       aTGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCtt                                              >  1:1038863/1‑71 (MQ=255)
                       aTGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCtt                                              >  1:541213/1‑71 (MQ=255)
                       aTGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCt                                               >  1:4539030/1‑70 (MQ=255)
                       aTGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCt                                               <  1:1710835/70‑1 (MQ=255)
                        tGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTg                                             <  1:1730479/71‑1 (MQ=255)
                        tGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTg                                             <  1:565600/71‑1 (MQ=255)
                         ggCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGa                                            >  1:1523487/1‑71 (MQ=255)
                               ttACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAag                                                                <  1:7476501/45‑1 (MQ=255)
                               ttACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGAc                                           >  1:690423/1‑66 (MQ=255)
                                tACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCaa                                     >  1:4563762/1‑71 (MQ=255)
                                  cccGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAAt                                    <  1:5981534/70‑1 (MQ=255)
                                  cccGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATg                                   >  1:2501168/1‑71 (MQ=255)
                                   ccGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATc                                                    <  1:5347267/53‑1 (MQ=255)
                                    cGTCGGCAAATATTCACTAAGCACTCTGATtaataata                                                                  <  1:4393642/38‑1 (MQ=255)
                                     gTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGAc                                                           >  1:2571865/1‑44 (MQ=255)
                                     gTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGAc                                           >  1:6087370/1‑60 (MQ=255)
                                     gTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATggg                                 >  1:5178502/1‑70 (MQ=255)
                                     gTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATggg                                 >  1:7174005/1‑70 (MQ=255)
                                      tCGGCAAATATTCACTAAGCACTCTGATtaataata                                                                  <  1:5860395/36‑1 (MQ=255)
                                      tCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCa                                                  <  1:2669736/52‑1 (MQ=255)
                                        ggCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATcc                                                   >  1:1102946/1‑49 (MQ=255)
                                        ggCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATcc                                                   >  1:4529146/1‑49 (MQ=255)
                                        ggCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTAtt                             <  1:6394192/71‑1 (MQ=255)
                                         gCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTa                            <  1:1655740/71‑1 (MQ=255)
                                          cAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTaa                           >  1:1729809/1‑71 (MQ=255)
                                             atatTCACTAAGCACTCTGATTAATAATAAGAGGACg                                                          >  1:3351133/1‑37 (MQ=255)
                                             atatTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATcc                                                   >  1:6753993/1‑44 (MQ=255)
                                             atatTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAAtt                         >  1:7687288/1‑70 (MQ=255)
                                              tatTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTg                        <  1:5363300/70‑1 (MQ=255)
                                              tatTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTg                        <  1:480247/70‑1 (MQ=255)
                                              tatTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGa                       <  1:6742164/71‑1 (MQ=255)
                                               atTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCAc                                                 >  1:6168038/1‑44 (MQ=255)
                                               atTCACTAAGAACTCTGATTAATAATAAGAGGACGCCAATCCAc                                                 >  1:3632851/1‑44 (MQ=255)
                                                ttCTCTAAGCACTCTGATTAATAATAAGAGGACGCCaa                                                      >  1:4387359/1‑38 (MQ=255)
                                                ttCACTAAGCACTCTGATTAATAATAAGAGGACGCCaa                                                      >  1:3933682/1‑38 (MQ=255)
                                                ttCACTAAGCACTCTGATTAATAATAAGAGGACGCCa                                                       >  1:502430/1‑37 (MQ=255)
                                                ttCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGAt                      >  1:4015155/1‑70 (MQ=255)
                                                ttCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATa                     <  1:2993381/71‑1 (MQ=255)
                                                ttCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATa                     <  1:6281227/71‑1 (MQ=255)
                                                   aCTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCa                                                  >  1:5011144/1‑39 (MQ=255)
                                                   aCTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCa                                                  >  1:1824553/1‑39 (MQ=255)
                                                    cTAAGCACTCTGATTAATAATAAGAGGACGCCAATcc                                                   <  1:4775864/37‑1 (MQ=255)
                                                    cTAAGCACTCTGATTAATAATAAGAGGACGCCAATcc                                                   <  1:1015146/37‑1 (MQ=255)
                                                    cTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCa                 <  1:1728878/71‑1 (MQ=255)
                                                     tAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTaa                           >  1:824630/1‑60 (MQ=255)
                                                     tAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCa                 >  1:3042880/1‑70 (MQ=255)
                                                       aGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCaa                <  1:7094284/69‑1 (MQ=255)
                                                       aGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAAc              <  1:7187802/71‑1 (MQ=255)
                                                       aGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAAc              <  1:6756769/71‑1 (MQ=255)
                                                       aGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAAc              >  1:6785068/1‑71 (MQ=255)
                                                        tcACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCa                 <  1:881609/66‑1 (MQ=255)
                                                        gCACTCTGATTAATAATAAGAGGACGCCAATCCACCt                                               <  1:7333575/37‑1 (MQ=255)
                                                        gCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCt                                         >  1:7804284/1‑43 (MQ=255)
                                                        gCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACt             >  1:3615279/1‑71 (MQ=255)
                                                         cACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTg            <  1:7680377/71‑1 (MQ=255)
                                                         cACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTg            <  1:2283672/71‑1 (MQ=255)
                                                          cctctTATTATTAATCAGAGGACGCCAATCCACCTTGAcc                                          <  1:1910433/39‑1 (MQ=18)
                                                          cctctTATTATTAATCAGAGGACGCCAATCCACCTTGAcc                                          <  1:2488377/39‑1 (MQ=18)
                                                          cctctTATTATTAATCAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTg            >  1:6117206/2‑70 (MQ=38)
                                                          cctctTATTATTAATCAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTg            >  1:5037863/2‑70 (MQ=38)
                                                          aCTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAAt                                    <  1:4408857/46‑1 (MQ=255)
                                                          aCTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTg            <  1:7459359/70‑1 (MQ=255)
                                                          aCTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGc           <  1:3689189/71‑1 (MQ=255)
                                                          aCTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGc           <  1:5188608/71‑1 (MQ=255)
                                                           ctctGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCa                                      <  1:6137558/43‑1 (MQ=255)
                                                           ctctGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTg            >  1:6376082/1‑69 (MQ=255)
                                                            tctGATTAATAATACGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTa                            <  1:6831114/52‑1 (MQ=255)
                                                            tctGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTAt                              <  1:2704093/50‑1 (MQ=255)
                                                            tctGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTAt                              <  1:3189671/50‑1 (MQ=255)
                                                            tctGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTa                            <  1:4174837/52‑1 (MQ=255)
                                                             ctGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGt        >  1:7799744/1‑71 (MQ=255)
                                                              tGATTAATAATAAGAGGACGCCAATCCACCTTGACCt                                         >  1:2771338/1‑37 (MQ=255)
                                                              tGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATgg                                  >  1:4612280/1‑44 (MQ=255)
                                                              tGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATgg                                  >  1:3554267/1‑44 (MQ=255)
                                                              tGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTa                            <  1:7508320/50‑1 (MQ=255)
                                                               gATTAATAATAAGAGGCCGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGttt      >  1:3130600/1‑71 (MQ=255)
                                                               gATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGttt      >  1:6428331/1‑71 (MQ=255)
                                                                aTTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGtt       <  1:3212968/69‑1 (MQ=255)
                                                                aTTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTg     <  1:7917759/71‑1 (MQ=255)
                                                                  taataataaGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCg   >  1:723771/1‑71 (MQ=255)
                                                                   aataataaGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAg         <  1:6521942/64‑1 (MQ=255)
                                                                   aataataaGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGt  >  1:4804719/1‑71 (MQ=255)
                                                                    ataataaGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGt  <  1:3028874/70‑1 (MQ=255)
                                                                    |                                                                     
TAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGT  >  W3110S.gb/3186163‑3186300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: