| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,449,061 | C→T | 5.3% | T757T (ACG→ACA) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,449,061 | 0 | C | T | 5.3% | 430.2 / 8.8 | 131 | T757T (ACG→ACA) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (71/52); new base T (2/5); total (73/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
ACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCTGTGCCG > W3110S.gb/3448992‑3449131 | cGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGt > 1:6594588/1‑71 (MQ=255) aGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:4489252/71‑1 (MQ=255) aGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:211584/71‑1 (MQ=255) gCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTc < 1:1559027/71‑1 (MQ=255) ccGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgat > 1:5696079/1‑71 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga < 1:5618265/71‑1 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga < 1:4805610/71‑1 (MQ=255) gggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGatgatg > 1:3400570/1‑71 (MQ=255) ggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGatgatg < 1:3059763/70‑1 (MQ=255) ttgagaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:3354661/58‑1 (MQ=255) gagaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatg > 1:5402488/1‑63 (MQ=255) agaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCa > 1:2817089/1‑71 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCg < 1:855146/53‑1 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCAt < 1:5055905/71‑1 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCAt < 1:3285446/71‑1 (MQ=255) gaggTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCg > 1:3405396/4‑71 (MQ=255) aacgTACTACAGTAC‑TTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCg > 1:4858885/4‑70 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCg < 1:4834278/70‑1 (MQ=255) gTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCt > 1:2819198/1‑71 (MQ=255) gTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCt < 1:6761310/71‑1 (MQ=255) tACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:7781136/49‑1 (MQ=255) tACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:1727769/49‑1 (MQ=255) tACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:5851906/49‑1 (MQ=255) tACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:4901691/49‑1 (MQ=255) cTGGAGTACGTTCGGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATcc < 1:4065550/64‑1 (MQ=255) cTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:170935/47‑1 (MQ=255) cTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg > 1:5703055/1‑49 (MQ=255) cTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATcc < 1:7416417/64‑1 (MQ=255) gAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGa < 1:5553744/54‑1 (MQ=255) gTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCAt > 1:7883882/1‑70 (MQ=255) tACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGc < 1:2734696/37‑1 (MQ=255) tACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGTGTTTCGATGATGGAGCCATCCGGCTTCGCCAt > 1:3988563/1‑69 (MQ=255) tACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTc > 1:369240/1‑64 (MQ=255) aCGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCAt > 1:1700237/1‑68 (MQ=255) cGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGTGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGa > 1:7932693/1‑71 (MQ=255) cGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCg > 1:2892166/1‑36 (MQ=255) cGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCg > 1:6548703/1‑36 (MQ=255) cGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGtt > 1:3161774/1‑38 (MQ=255) cGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGtt > 1:5380157/1‑38 (MQ=255) gTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCAt < 1:4116256/66‑1 (MQ=255) gTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGa > 1:5278114/1‑70 (MQ=255) ttCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGc < 1:411106/49‑1 (MQ=255) tCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCa > 1:5403461/1‑71 (MQ=255) tCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCa > 1:2338884/1‑71 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCAt < 1:6832132/49‑1 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCAt < 1:7196915/49‑1 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATcc > 1:4764928/1‑51 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGa > 1:2419108/1‑66 (MQ=255) gACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATcc > 1:2851704/1‑50 (MQ=255) gACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATcc > 1:7330932/1‑50 (MQ=255) gACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCtt > 1:4311700/1‑55 (MQ=255) gACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCt > 1:1631124/1‑54 (MQ=255) gACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCAc > 1:321545/1‑69 (MQ=255) gACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCAc > 1:1590097/1‑69 (MQ=255) ccTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCAt < 1:1168661/71‑1 (MQ=255) cTTCACGGAAGTTCGCGGTGATTGGAGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACg < 1:5288200/67‑1 (MQ=255) tCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATAc > 1:6729138/1‑70 (MQ=255) tCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATAc > 1:180041/1‑70 (MQ=255) cACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGTGCCATCCGGCTTCGCCATCa > 1:5533281/1‑57 (MQ=255) cACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCAtaa > 1:6946680/1‑55 (MQ=255) cACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGa > 1:1501321/1‑59 (MQ=255) cACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATAc > 1:4804969/1‑69 (MQ=255) aCGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAg > 1:2069629/1‑37 (MQ=255) aCGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAg > 1:318445/1‑71 (MQ=255) aCGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAg > 1:5846720/1‑71 (MQ=255) ggAAGTTCGCGGTGATTGGTGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCa < 1:4510510/71‑1 (MQ=255) ggAAGTTCGCGGTGATTGGTGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCa < 1:4509588/71‑1 (MQ=255) ggAAGTTCGCGGTGATTGGTGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCa < 1:6199303/71‑1 (MQ=255) ggAAGTTCGCGGTGATTGGTGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCa < 1:3413741/71‑1 (MQ=255) ggAAGTTCGCGGTGATTGGTGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCa < 1:6716236/71‑1 (MQ=255) ggAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGc > 1:7446772/1‑70 (MQ=255) gAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATAc > 1:3106343/1‑65 (MQ=255) gAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCaa < 1:3895425/71‑1 (MQ=255) aaGTTCGCGGTGATTTGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGa > 1:7307000/1‑54 (MQ=255) aGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCgg > 1:5827682/1‑40 (MQ=255) aGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGa > 1:2690202/1‑53 (MQ=255) gTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCt < 1:820396/71‑1 (MQ=255) ttCGCGGTTATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTg < 1:6211507/71‑1 (MQ=255) ttCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTg < 1:5113429/71‑1 (MQ=255) tCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGa > 1:2345178/1‑71 (MQ=255) ggTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCAt < 1:7729207/54‑1 (MQ=255) ggTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGaa > 1:1923901/1‑71 (MQ=255) ggTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGAATACCAGCAAGCTGACGa > 1:3294459/1‑70 (MQ=255) gTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAAt < 1:6571312/71‑1 (MQ=255) gATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCa > 1:2629437/1‑50 (MQ=255) aTTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCAt < 1:2356105/50‑1 (MQ=255) aTTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAATCTg > 1:3048797/1‑62 (MQ=255) aTTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTg > 1:2954820/1‑62 (MQ=255) aTTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTg > 1:3944516/1‑62 (MQ=255) aTTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCtg < 1:3044854/71‑1 (MQ=255) aTTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCtg > 1:738776/1‑71 (MQ=255) aTTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCtg > 1:5667763/1‑71 (MQ=255) aTTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCtg < 1:5144955/71‑1 (MQ=255) aTTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCt < 1:2534179/70‑1 (MQ=255) aTTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCt < 1:5184609/70‑1 (MQ=255) ttGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCa > 1:2430578/1‑44 (MQ=255) ttGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCa > 1:6190551/1‑44 (MQ=255) ttGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCa > 1:4194275/1‑44 (MQ=255) ttGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCtgt > 1:6941356/1‑71 (MQ=255) tGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGc < 1:2107099/54‑1 (MQ=255) | ACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCTGTGCCG > W3110S.gb/3448992‑3449131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |