| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,811,561 | A→G | 5.4% | intergenic (‑89/‑191) | yicE ← / → gltS | predicted transporter/glutamate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,811,561 | 0 | A | G | 5.4% | 318.6 / 7.1 | 111 | intergenic (‑89/‑191) | yicE/gltS | predicted transporter/glutamate transporter |
| Reads supporting (aligned to +/- strand): ref base A (43/62); new base G (3/3); total (46/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
GCAAAGCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGTTCA > W3110S.gb/3811492‑3811629 | gCAAAGCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTaa < 1:5634923/70‑1 (MQ=255) aaaGCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAAt < 1:85224/70‑1 (MQ=255) aaaGCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAAt < 1:5180893/70‑1 (MQ=255) aaGCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATTTAAATATTTAGATga < 1:5690791/71‑1 (MQ=255) aGCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATTTAAATATTTAGATgag < 1:3844468/71‑1 (MQ=255) aGCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAAt > 1:872571/1‑68 (MQ=255) gCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAAt > 1:4441646/1‑67 (MQ=255) ggCATTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATaa < 1:794122/71‑1 (MQ=255) aTTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGAt < 1:1313405/66‑1 (MQ=255) ttttAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAAt > 1:4588579/1‑69 (MQ=255) ttAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTaaa > 1:6850614/1‑57 (MQ=255) ttAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCAc > 1:27716/1‑71 (MQ=255) ttAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCAc > 1:2392714/1‑71 (MQ=255) tAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACt > 1:3267369/1‑71 (MQ=255) cTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACtttt < 1:29647/71‑1 (MQ=255) atGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACtttt < 1:4437269/70‑1 (MQ=255) gACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATTTAAATATTTAGATgagg > 1:349819/1‑55 (MQ=37) aCCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACtttttt > 1:4786131/1‑70 (MQ=255) aCCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACtttttt < 1:1637742/70‑1 (MQ=255) aCCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACtttttt < 1:6855041/70‑1 (MQ=255) aCCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACttttt < 1:2084217/69‑1 (MQ=255) aCCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTta > 1:3583205/1‑71 (MQ=255) ccGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTtat < 1:682212/71‑1 (MQ=255) cGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTtata > 1:7590425/1‑71 (MQ=255) cGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTtata > 1:1369232/1‑71 (MQ=255) agagAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAAc > 1:40938/1‑71 (MQ=255) gagaAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACt < 1:6739864/71‑1 (MQ=255) gagaAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACt < 1:450284/71‑1 (MQ=255) agaAAAGGAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGAt < 1:5584399/48‑1 (MQ=255) agaAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAAt < 1:7373077/43‑1 (MQ=255) agaAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAAt < 1:3766900/43‑1 (MQ=255) agaAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGAt < 1:3410964/48‑1 (MQ=255) aaaaGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATaa > 1:4586311/1‑48 (MQ=255) aaaaGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACtttt < 1:1608759/71‑1 (MQ=255) aaGCAAACGTTTGCCACATCCTCATCTAAATATTTaaa < 1:1623639/38‑1 (MQ=255) aaGCAAACGTTTGCCACATCCTCATCTAAATATTTaaa < 1:364691/38‑1 (MQ=255) aGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACttttt < 1:4123662/56‑1 (MQ=255) gCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTa > 1:2462995/1‑71 (MQ=255) cAAACGTTTGCCACATCCTCATCTAAATATTTAAAt < 1:3688609/36‑1 (MQ=255) aaCGTTTGCCACATCCTCATCTAAATATTTAAATGCGATAAATGCACTTTTTTATATAACTTTTGGTAttt < 1:7090281/71‑1 (MQ=255) aaCGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAttt < 1:1931544/71‑1 (MQ=255) aaCGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAttt < 1:5183817/71‑1 (MQ=255) aaCGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAttt < 1:4962323/71‑1 (MQ=255) aaCGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAttt < 1:4367173/71‑1 (MQ=255) aCGTTTGCCACATCCTCATCTAAATATTTAAATGAGATa < 1:7456184/39‑1 (MQ=255) aCGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAtttt > 1:6464803/1‑71 (MQ=255) aCGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAtttt > 1:7631917/1‑71 (MQ=255) cGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTta > 1:1141670/1‑52 (MQ=255) cGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACttt < 1:2364790/61‑1 (MQ=255) cGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACttt < 1:5808229/61‑1 (MQ=255) tttGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCt > 1:5947568/1‑71 (MQ=255) tGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGc > 1:2227630/1‑71 (MQ=255) ccACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAttt < 1:6236464/63‑1 (MQ=255) ccACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAttt < 1:7744287/63‑1 (MQ=255) cacaTCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACttt < 1:5580297/54‑1 (MQ=255) cacaTCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCtt > 1:2561906/1‑70 (MQ=255) acctCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTa > 1:6723657/4‑70 (MQ=255) aTCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTaaaa < 1:2213675/71‑1 (MQ=255) aTCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTaaaa < 1:6216874/71‑1 (MQ=255) tCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTta > 1:1789673/1‑41 (MQ=255) tCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTta > 1:5992712/1‑41 (MQ=255) tCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTta > 1:3156231/1‑41 (MQ=255) tCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACtttt > 1:3525991/1‑51 (MQ=255) tCCTCATCTAAATATTTAAATGAGATAAATGCAATTTTTta > 1:6573971/1‑41 (MQ=255) cTCATTTAAATATTTAGATGAGATAAATGCACTTTTTtatat > 1:1520324/1‑42 (MQ=25) cTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATc < 1:7094620/70‑1 (MQ=255) tCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTa < 1:7502930/52‑1 (MQ=255) aTCTAAATATTTAAATGAGATAAATGCACTTTTTtat < 1:3543388/37‑1 (MQ=255) aTCTAAATATTTAAATGAGATAAATGCACTTTTTtat > 1:2568558/1‑37 (MQ=255) aTCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAtt > 1:1593414/1‑52 (MQ=255) aTCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCa < 1:494231/69‑1 (MQ=255) aTCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCa < 1:2604623/69‑1 (MQ=255) aTCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCAt < 1:816902/70‑1 (MQ=255) aTCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATg > 1:2183599/1‑71 (MQ=255) cTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTaaaa < 1:5690549/63‑1 (MQ=255) cTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGc > 1:333324/1‑70 (MQ=255) tAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCa > 1:7383380/1‑71 (MQ=255) aaaTATTTAAATGAGATAAATGCACTTTTTTATATAACttt < 1:1061193/41‑1 (MQ=255) aaaTATTTAAATGAGATAAATGCACTTTTTTATATAACttt < 1:1047953/41‑1 (MQ=255) aaaTATTTAAATGAGATAAATGCACTTTTTTATATAACttt < 1:6538357/41‑1 (MQ=255) aaaTATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCAt > 1:3644596/1‑71 (MQ=255) aaaTATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCAt > 1:7538883/1‑71 (MQ=255) aaTATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAttt < 1:2364927/48‑1 (MQ=255) aaTATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCa < 1:309846/69‑1 (MQ=255) aaTATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCAtt > 1:6740363/1‑71 (MQ=255) aaTATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCAt < 1:6829654/70‑1 (MQ=255) atatTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAttt < 1:1578998/47‑1 (MQ=255) atatTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTAttt < 1:2809548/47‑1 (MQ=255) tatTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTg < 1:5120981/71‑1 (MQ=255) tttAGATGAGATAAATGCACTTTTTTATATAACTTTTGGTAttttt > 1:6674992/1‑46 (MQ=255) tttAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGt < 1:3611920/70‑1 (MQ=255) tttAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGt < 1:3779574/70‑1 (MQ=255) tttAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGt < 1:3183542/70‑1 (MQ=255) tttAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGt < 1:1311097/70‑1 (MQ=255) tttAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGt < 1:5678002/70‑1 (MQ=255) tttAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGt < 1:6351045/70‑1 (MQ=255) tAAATGAGATAAATGCACTTTTTTATATAACTTTTg > 1:7061704/1‑36 (MQ=255) tAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGTTc < 1:5892911/70‑1 (MQ=255) aaaTGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGTTc < 1:7818299/69‑1 (MQ=255) aaTGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGTTCa < 1:7673729/69‑1 (MQ=255) | GCAAAGCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAACGTTTGCCACATCCTCATCTAAATATTTAAATGAGATAAATGCACTTTTTTATATAACTTTTGGTATTTTTCTGCTTAAAATCCATGCCATTTGTTCA > W3110S.gb/3811492‑3811629 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |