| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,985,586 | T→A | 5.4% | N50I (AAT→ATT) | yhiF ← | predicted DNA‑binding ranscriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,985,586 | 0 | T | A | 5.4% | 428.3 / 12.5 | 149 | N50I (AAT→ATT) | yhiF | predicted DNA‑binding ranscriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (71/70); new base A (5/3); total (76/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
AGAAAATAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGTAT > W3110S.gb/3985518‑3985656 | agaAAATAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTc < 1:1953217/71‑1 (MQ=255) aaaaTAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCAt < 1:2505885/71‑1 (MQ=255) taatGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTaa > 1:6379511/1‑70 (MQ=255) taatGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTaa > 1:6773452/1‑70 (MQ=255) taatGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAAt < 1:6227926/71‑1 (MQ=255) taatGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAAt < 1:7170197/71‑1 (MQ=255) taatGACATGGACAGGTTTTAATAGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAAt > 1:6322599/1‑71 (MQ=255) atGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATg > 1:1912963/1‑70 (MQ=255) atGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATg > 1:717595/1‑70 (MQ=255) tGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGt > 1:3539366/1‑70 (MQ=255) tGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaat < 1:2852621/71‑1 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTa > 1:501894/1‑58 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaataat < 1:6107411/71‑1 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaataat < 1:5314264/71‑1 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaataat < 1:6283109/71‑1 (MQ=255) cAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATg < 1:6733921/71‑1 (MQ=255) aGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTATTGATACATTAATGa < 1:3201127/71‑1 (MQ=37) ggTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGAc > 1:4307000/1‑71 (MQ=255) gTTTTAATCGTTCAATTTTAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaata < 1:5217415/65‑1 (MQ=255) gTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGAc > 1:5177844/1‑70 (MQ=255) gTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACg < 1:6771886/71‑1 (MQ=255) ttttAATCGTTCAATTTGAGTGAGAAGATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGa < 1:537070/71‑1 (MQ=255) ttttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACg < 1:4667897/70‑1 (MQ=255) ttttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGa < 1:5148182/71‑1 (MQ=255) ttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACattat < 1:1998722/45‑1 (MQ=255) ttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGaa < 1:1774467/70‑1 (MQ=255) ttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAg > 1:3510799/1‑71 (MQ=255) tAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaata < 1:903626/61‑1 (MQ=255) aaTCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCa < 1:3342727/46‑1 (MQ=255) aaTCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAgg > 1:4896443/1‑70 (MQ=255) aTCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCa < 1:7301542/71‑1 (MQ=255) tCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCAt > 1:5919592/1‑71 (MQ=255) tCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCAt < 1:129538/71‑1 (MQ=255) tCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCAt > 1:3079096/1‑71 (MQ=255) tCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCAt > 1:2694633/1‑71 (MQ=255) gTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTc > 1:408861/1‑71 (MQ=255) gTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTc > 1:4675888/1‑71 (MQ=255) ttCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTAt < 1:3932863/50‑1 (MQ=255) cAATTTGAGTGAGAAAATTAGAATGAAATACATTATTc > 1:5662481/1‑38 (MQ=255) aaTTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGa < 1:7844567/70‑1 (MQ=255) aaTTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGAt > 1:3886724/1‑71 (MQ=255) aaTTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGAt > 1:6369991/1‑71 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCAtt < 1:5352809/39‑1 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCAtt < 1:2114608/39‑1 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCAtt < 1:82653/39‑1 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCAtt < 1:4331105/39‑1 (MQ=255) tttGAGTGAGAAAATTAGAATTAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGc < 1:5762774/71‑1 (MQ=255) tttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGaa > 1:3810045/1‑58 (MQ=255) tttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGaa > 1:93267/1‑58 (MQ=255) tttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGaa > 1:5141966/1‑58 (MQ=255) tttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATg > 1:3696619/1‑70 (MQ=255) tttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGc < 1:4040635/71‑1 (MQ=255) ttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaa > 1:3848210/1‑47 (MQ=255) ttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaa > 1:6637949/1‑47 (MQ=255) tgagtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCaa > 1:1623621/1‑71 (MQ=255) gagtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCaa < 1:2940259/70‑1 (MQ=255) agtgagAAAATTAGAATGAAATACATTATTCATTAATGt < 1:5974578/39‑1 (MQ=255) agtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAAc < 1:2149265/70‑1 (MQ=255) agtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACa < 1:2951635/71‑1 (MQ=255) agtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACa < 1:4325264/71‑1 (MQ=255) agtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACa < 1:283733/71‑1 (MQ=255) gtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCaat < 1:1850129/44‑1 (MQ=255) gtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCAtt > 1:3971425/1‑59 (MQ=255) gagaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGa < 1:3637760/50‑1 (MQ=255) agaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTa < 1:6857613/70‑1 (MQ=255) agaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTAc < 1:614123/71‑1 (MQ=255) agaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTAc < 1:5629336/71‑1 (MQ=255) gaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCt < 1:1255461/57‑1 (MQ=255) aaaaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTTATGCAACATTACGt > 1:4562964/1‑71 (MQ=255) aaaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTc < 1:1893446/71‑1 (MQ=255) aaTTAGAATGACATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCg < 1:273478/71‑1 (MQ=255) aaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGaa > 1:939367/1‑46 (MQ=255) aaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCg < 1:5526613/71‑1 (MQ=255) ttAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCTTTCTGATGCAACATTACGTCg > 1:5115844/1‑69 (MQ=255) gAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGa > 1:5767621/1‑51 (MQ=255) tGAAATACATTATTCATTAATGTATCAATAATGACTAAGGCATTCTGATGCAACATTACGTCGATCtcttc > 1:7556475/1‑71 (MQ=255) tGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAt > 1:1722611/1‑65 (MQ=255) tGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttc > 1:5250918/1‑71 (MQ=255) tGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttc > 1:1135501/1‑71 (MQ=255) tgaTACATTAATGAATAATGTATTAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAtctc > 1:1537633/3‑66 (MQ=25) tgaTACATTAATGAATAATGTATTAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAtctc > 1:3338056/3‑66 (MQ=25) tgaTACATTAATGAATAATGTATTAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAtctc > 1:3027315/3‑66 (MQ=25) aaaTACATTATTCATTAATGTATCAATAATGACGAAGGCAt < 1:3125217/41‑1 (MQ=255) gaTACATTAATGAATAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTAc < 1:2383549/55‑1 (MQ=37) aaTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCttcttc > 1:2405044/1‑71 (MQ=255) aaTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCttcttc > 1:7549727/1‑71 (MQ=255) aTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATg > 1:7860438/1‑46 (MQ=255) aTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCg < 1:4009303/71‑1 (MQ=255) aTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCg > 1:5384949/1‑71 (MQ=255) aTACATTAATGAATAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttc < 1:890980/67‑1 (MQ=38) aCATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGtt > 1:6996859/1‑71 (MQ=255) aCATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGt > 1:955632/1‑70 (MQ=255) aCATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGt > 1:389942/1‑70 (MQ=255) cATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGAt < 1:3768597/42‑1 (MQ=255) cATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTc < 1:3716232/55‑1 (MQ=255) cATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTc > 1:7022627/1‑71 (MQ=255) tattCATTAATGTATCAATAATGACGAAGGCATTCt < 1:2200689/36‑1 (MQ=255) ttCATTAATGTATCAATAATGACGAAGGCATTCTGa < 1:2014163/36‑1 (MQ=255) ttCATTAATGTATCAATAATGACGAAGGCATTCTGa < 1:4269505/36‑1 (MQ=255) ttCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGt < 1:5962736/69‑1 (MQ=255) | AGAAAATAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGTAT > W3110S.gb/3985518‑3985656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |