| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,009,464 | G→T | 7.7% | intergenic (+15/‑351) | yhiJ → / → yhiI | hypothetical protein/predicted HlyD family secretion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,009,464 | 0 | G | T | 7.7% | 538.4 / 14.7 | 165 | intergenic (+15/‑351) | yhiJ/yhiI | hypothetical protein/predicted HlyD family secretion protein |
| Reads supporting (aligned to +/- strand): ref base G (80/72); new base T (6/7); total (86/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 166 total aligned reads displayed.
AAACAGAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTGTTCCTGCA > W3110S.gb/4009395‑4009530 | aaaCAGAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGa < 1:4736208/71‑1 (MQ=255)aaaCAGAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGa < 1:7737587/71‑1 (MQ=255) aaCAGAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAAGATGaa > 1:1611544/1‑71 (MQ=255) aaCAGAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGaa > 1:2056058/1‑71 (MQ=255) aaCAGAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGaa > 1:5850432/1‑71 (MQ=255) cAGAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAata > 1:3405204/1‑71 (MQ=255) gAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATTAatat > 1:7605599/1‑70 (MQ=255) gAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAatat < 1:5874715/70‑1 (MQ=255) gAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATAtt < 1:2221465/71‑1 (MQ=255) aaaTGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATTAATAtt > 1:824002/1‑70 (MQ=255) aaaTGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATTAATAtt > 1:3716437/1‑70 (MQ=255) aaaTGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATTAATAtt > 1:2969460/1‑70 (MQ=255) aaTGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATTAATAttca < 1:4327456/71‑3 (MQ=255) aTGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTaa > 1:2041687/1‑70 (MQ=255) aTGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAAt > 1:2499674/1‑71 (MQ=255) tGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATTAATAttca < 1:6666903/69‑3 (MQ=255) tGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATg > 1:556836/1‑71 (MQ=255) gACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGt > 1:2237067/1‑70 (MQ=255) cTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTa < 1:7766044/70‑1 (MQ=255) cTTATGCCTTTGATAAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTa < 1:5175822/70‑1 (MQ=255) ttATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGAGAACATGAATATTAATGTTAc > 1:90540/1‑70 (MQ=255) ttATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGtt < 1:5123091/68‑1 (MQ=255) ttATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGtt < 1:1866587/68‑1 (MQ=255) ttATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGtt < 1:1141426/68‑1 (MQ=255) tGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATc < 1:5646345/71‑1 (MQ=255) cTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGt < 1:6909852/61‑1 (MQ=255) cTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGt < 1:3157293/61‑1 (MQ=255) cTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTgg < 1:4891421/71‑1 (MQ=255) cTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTgg < 1:5219193/71‑1 (MQ=255) tttGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGt > 1:408144/1‑71 (MQ=255) ttGATTAGAAGGCGCACAATGTTACAGCTCTGCACCATAGACAACATGAATATTAATGTTACTATCTGGtt > 1:3940645/1‑71 (MQ=255) ttGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGt > 1:3494408/1‑70 (MQ=255) gATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGtt > 1:6993654/1‑69 (MQ=255) aGAAGGCGCACAATGTTACAGCTCTGAACCTTAGACAACATGAATATTAATGt > 1:1447044/1‑53 (MQ=255) aGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAata > 1:4358054/1‑46 (MQ=255) aGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGt > 1:6226257/1‑53 (MQ=255) aGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGt > 1:1988267/1‑53 (MQ=255) aGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGt > 1:7213509/1‑53 (MQ=255) aGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACt > 1:2764257/1‑71 (MQ=255) gAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTg < 1:1519007/71‑1 (MQ=255) cacaATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATaa < 1:3963578/71‑1 (MQ=255) acaATGTTACAGCTCTGAACCATAGACAACATGAat > 1:346621/1‑36 (MQ=255) acaATGTTACAGCTCTGAACCATAGACAACATGAat > 1:2652070/1‑36 (MQ=255) acaATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTgaga > 1:3308317/1‑60 (MQ=255) acaATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATaa > 1:2179835/1‑70 (MQ=255) acaATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATaa < 1:7928381/70‑1 (MQ=255) aaTGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCt > 1:2982456/1‑50 (MQ=255) aaTGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCt > 1:7509050/1‑50 (MQ=255) aaTGTTACAGCTATGAACCATAGACAACTTTAATATTCATGttg > 1:2967930/1‑43 (MQ=25) aTGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAAc < 1:4809577/70‑1 (MQ=255) aTGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACg < 1:702550/71‑1 (MQ=255) aTGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACg < 1:1149292/71‑1 (MQ=255) gTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGt > 1:4046440/1‑70 (MQ=255) aCAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTga > 1:567133/1‑50 (MQ=255) aCAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTga > 1:4206307/1‑50 (MQ=255) aCAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTga > 1:4196419/1‑50 (MQ=255) aCAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTaaa < 1:5050040/70‑1 (MQ=255) aGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAg < 1:2112765/71‑1 (MQ=255) aGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAg < 1:2470329/71‑1 (MQ=255) aGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAg > 1:1379036/1‑71 (MQ=255) gCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATaaa < 1:3283781/60‑1 (MQ=255) ctctGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAGAAgg > 1:6111063/1‑70 (MQ=255) tctGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTga < 1:822840/45‑1 (MQ=255) tctGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAAc > 1:6582176/1‑60 (MQ=255) tctGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAAc > 1:3287487/1‑60 (MQ=255) tctGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAAc > 1:3059337/1‑60 (MQ=255) ctGAACCATAGACAACATGAATATTAATGTTACTATCTg < 1:3744224/39‑1 (MQ=255) tGAACCATAGAGAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGt < 1:2135693/60‑1 (MQ=255) tGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGt < 1:7586740/60‑1 (MQ=255) aaCCATAGACAACATGAATATTAATGTTACTATCTGGTTgaga > 1:4711421/1‑43 (MQ=255) aaCCATAGACAACATGAATATTAATGTTACTATCTGGTTgaga > 1:6425454/1‑43 (MQ=255) aaCCATAGACAACATGAATATTAATGTTACTATCTGGTTgaga > 1:2382277/1‑43 (MQ=255) aaCCATAGACAACATGAATATTAATGTTACTATCTGGTTgaga > 1:2667995/1‑43 (MQ=255) aaCCATAGACAACATGAATATTAATGTTACTATCTGGTTgag > 1:7318844/1‑42 (MQ=255) aaCCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGt < 1:6051349/47‑1 (MQ=255) aaCCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAAt < 1:6663880/68‑1 (MQ=255) aCCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGctc > 1:2896842/1‑71 (MQ=255) aCCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGct < 1:3025550/70‑1 (MQ=255) ccATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGt > 1:2261211/1‑45 (MQ=255) ccATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGt > 1:1120337/1‑45 (MQ=255) ccATAGACAACATGAATATTAATGTTACTA‑‑TGGTTGAGACTGt > 1:113777/1‑43 (MQ=37) cATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGt > 1:1475652/1‑44 (MQ=255) cATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGt > 1:183375/1‑44 (MQ=255) cATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGt > 1:7056165/1‑44 (MQ=255) cATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGAt < 1:1683803/48‑1 (MQ=255) cATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGAt < 1:4989335/48‑1 (MQ=255) cATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGAt < 1:491387/48‑1 (MQ=255) cATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTg < 1:1535419/71‑1 (MQ=255) cATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTg < 1:2355665/71‑1 (MQ=255) aTAGACAACATTAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTGt < 1:5174878/71‑1 (MQ=255) aTAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTGt > 1:4385791/1‑71 (MQ=255) tAGACAACATTAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTGt > 1:4845021/1‑70 (MQ=255) tAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTGtt < 1:1972826/71‑1 (MQ=255) tAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTGtt < 1:7464048/71‑1 (MQ=255) tAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTGtt < 1:5051978/71‑1 (MQ=255) aGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTGTTc > 1:4094233/1‑71 (MQ=255) acaacaTGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAg < 1:3871570/56‑1 (MQ=255) acaacaTGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTGTTACt > 1:5912935/1‑71 (MQ=255) caTGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTg > 1:1315497/1‑62 (MQ=255) caTGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTg > 1:6467455/1‑62 (MQ=255) | AAACAGAAATGGACTTATGCCTTTGATTAGAAGGCGCACAATGTTACAGCTCTGAACCATAGACAACATGAATATTAATGTTACTATCTGGTTGAGACTGTTGATAAAACGTAAAAAGGAATGCTCTGTTCCTGCA > W3110S.gb/4009395‑4009530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |