| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,012,884 | C→T | 5.9% | T669M (ACG→ATG) | rbbA → | fused ribosome‑associated ATPases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,012,884 | 0 | C | T | 5.9% | 431.4 / 10.4 | 134 | T669M (ACG→ATG) | rbbA | fused ribosome‑associated ATPases |
| Reads supporting (aligned to +/- strand): ref base C (55/71); new base T (2/6); total (57/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 140 total aligned reads displayed.
GATATCGCGCGTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGCCGACAATCGACAC > W3110S.gb/4012814‑4012952 | gATATCGCGCGTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAAc > 1:858816/1‑71 (MQ=255)gATATCGCGCGTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAAc < 1:4313291/71‑1 (MQ=255)gATATCGCGCGTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAAc > 1:5129890/1‑71 (MQ=255)gATATCGCGCGTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAAc < 1:2757668/71‑1 (MQ=255) tCGCGCGTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGt < 1:5242960/70‑1 (MQ=255) gcgcgTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTaa < 1:2004481/70‑1 (MQ=255) cgcgTGGTACGCCTGTGGAACTCGTCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTaaaa < 1:4117553/71‑1 (MQ=255) cgcgTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTaaaa < 1:7114215/71‑1 (MQ=255) gcgTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAg > 1:7873219/1‑71 (MQ=255) gTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGt > 1:5512039/1‑71 (MQ=255) gTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGATCCGTGCTGAAACGGTAAAAGGTTAc < 1:2601946/71‑1 (MQ=255) gTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTAc < 1:3853381/71‑1 (MQ=255) tACGCCTGTGGAACTCGGCGTCTGGATCGACGGCGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTAc > 1:7772424/1‑70 (MQ=255) tACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAg > 1:5143004/1‑65 (MQ=255) tACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACg > 1:1121047/1‑71 (MQ=255) tACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACg > 1:5905488/1‑71 (MQ=255) tACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACg > 1:7052904/1‑71 (MQ=255) cTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGc > 1:4588608/1‑69 (MQ=255) cTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAg > 1:4217221/1‑71 (MQ=255) cTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAg > 1:4025661/1‑71 (MQ=255) gtgGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGc > 1:1513027/1‑71 (MQ=255) tgGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAgg < 1:413864/69‑1 (MQ=255) tgGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAgg < 1:2012863/69‑1 (MQ=255) tgGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGcc < 1:7498771/71‑1 (MQ=255) gAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAgg > 1:6643409/1‑67 (MQ=255) aaCTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATg < 1:311883/71‑1 (MQ=255) cTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCa < 1:5694358/71‑1 (MQ=255) cTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCa < 1:582779/71‑1 (MQ=255) tCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGt < 1:249148/58‑1 (MQ=255) ggCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTAc > 1:6598602/1‑54 (MQ=255) ggCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCAc > 1:7478230/1‑69 (MQ=255) ggCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCAc > 1:1074774/1‑69 (MQ=255) ggCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCAc < 1:1289444/69‑1 (MQ=255) ggCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCAc > 1:3551673/1‑69 (MQ=255) ggCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCAc > 1:3114751/1‑69 (MQ=255) gCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCa < 1:1609469/70‑1 (MQ=255) cTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAgg > 1:543881/1‑56 (MQ=255) tGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCt > 1:6043238/1‑70 (MQ=255) ggATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAgg < 1:2793488/54‑1 (MQ=255) gATCGACGGAGCGATGCCGAGCCGTTCTGAAATGGTAAAAGGTTACGTGCAGGCCAt < 1:3763670/57‑1 (MQ=255) gATCGACGGAGCGATGCCGAGCCGTGCTGAAATGGTAAAAGGTTACGTGCAGGCCAt < 1:5203697/57‑1 (MQ=255) gATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGc < 1:854540/54‑1 (MQ=255) gATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGc < 1:5727374/54‑1 (MQ=255) gATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGc < 1:6935829/54‑1 (MQ=255) gATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTg > 1:3490974/1‑69 (MQ=255) aTCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGt < 1:3649136/42‑1 (MQ=255) aTCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGtt < 1:1025488/71‑1 (MQ=255) aTCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGtt < 1:2703091/71‑1 (MQ=255) tCGACGGAGCGATGCCGAGCCGTGCTGAAATGGTAAAAGGTTACGTGCAGGCCATGc > 1:2568680/1‑57 (MQ=255) tCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCa > 1:4687795/1‑54 (MQ=255) tCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCa > 1:5816690/1‑54 (MQ=255) cGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCa > 1:4795446/1‑48 (MQ=255) cGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCaga > 1:3993082/1‑62 (MQ=255) cGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTa > 1:1522855/1‑70 (MQ=255) gACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGc < 1:6036605/50‑1 (MQ=255) gACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGc < 1:1483766/50‑1 (MQ=255) gACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTAc < 1:7888612/70‑1 (MQ=255) cGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAg > 1:5033735/1‑70 (MQ=255) ggAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCagag > 1:7604710/1‑59 (MQ=255) ggAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGa > 1:4492938/1‑71 (MQ=255) gCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTAc < 1:7307642/36‑1 (MQ=255) gCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTAc < 1:1310478/36‑1 (MQ=255) gCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTAc < 1:5511925/36‑1 (MQ=255) gCGATGCC‑AGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGAtgtg > 1:3735057/1‑71 (MQ=255) tGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAg < 1:6115686/62‑1 (MQ=255) gCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGt > 1:4724470/1‑56 (MQ=255) ccGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAg > 1:7280220/1‑71 (MQ=255) cGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGtt > 1:3474374/1‑55 (MQ=255) cGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTAGCGAGc < 1:1619493/71‑1 (MQ=255) gAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGcc < 1:6584360/71‑1 (MQ=255) gAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGcc < 1:5594355/71‑1 (MQ=255) gAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGc < 1:4046055/70‑1 (MQ=255) aGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGCCg < 1:5018299/71‑1 (MQ=255) gCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCag > 1:5374132/1‑44 (MQ=255) gCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCag > 1:4810195/1‑44 (MQ=255) cGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGCCGAc < 1:6109208/70‑1 (MQ=255) gTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGcc < 1:3216061/66‑1 (MQ=255) gTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGcc < 1:2754213/66‑1 (MQ=255) cTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTAc > 1:7407039/1‑48 (MQ=255) cTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTAc > 1:6861411/1‑48 (MQ=255) cTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGCCGACAATc > 1:2380920/1‑70 (MQ=255) gAAACGGTAAAAGGTTACGTGCATGCCATGCACCag < 1:2140593/36‑1 (MQ=255) gAAACGGTAAAAGGTTACGTGCAGGCCATGCACCag < 1:7402112/36‑1 (MQ=255) gAAACGGTAAAAGGTTACGTGCAGGCCATGCACCag < 1:330143/36‑1 (MQ=255) gAAACGGTAAAAGGTTACGTGCAGGCCATGCACCag < 1:5561500/36‑1 (MQ=255) gAAACGGTAAAAGGTTACGTGCAGGCCATGCACCag < 1:5344953/36‑1 (MQ=255) gAAACGGTAAAAGGTTACGTGCAGGCCATGCACCag < 1:3692814/36‑1 (MQ=255) gAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAgg < 1:4087424/49‑1 (MQ=255) gAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAgg < 1:5047703/49‑1 (MQ=255) aaaCGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGc < 1:3229627/38‑1 (MQ=255) aaaCGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGAtgt < 1:3846581/52‑1 (MQ=255) aaaCGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGCCGACAATCGa < 1:3901776/69‑1 (MQ=255) aaCGGTAAAAGGTTACGTGCAGGCCATGCACCagag > 1:634486/1‑36 (MQ=255) aaCGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGCCGACAATCGacac < 1:4557821/71‑1 (MQ=255) aaCGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGCCGACAATCGacac < 1:52497/71‑1 (MQ=255) aaCGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGCCGACAATCGacac < 1:3990900/71‑1 (MQ=255) aCGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCta > 1:1801995/1‑37 (MQ=255) aCGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTg > 1:2729575/1‑38 (MQ=255) aCGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTg > 1:5667573/1‑38 (MQ=255) aCGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTg > 1:4669330/1‑38 (MQ=255) | GATATCGCGCGTGGTACGCCTGTGGAACTCGGCGTCTGGATCGACGGAGCGATGCCGAGCCGTGCTGAAACGGTAAAAGGTTACGTGCAGGCCATGCACCAGAGCTGGTTACAGGATGTGGCGAGCCGACAATCGACAC > W3110S.gb/4012814‑4012952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |