| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,119,901 | A→T | 6.5% | T63S (ACA→TCA) ‡ | hofQ → | predicted fimbrial transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,119,901 | 0 | A | T | 6.5% | 433.7 / 17.9 | 153 | T63S (ACA→TCA) ‡ | hofQ | predicted fimbrial transporter |
| Reads supporting (aligned to +/- strand): ref base A (69/74); new base T (4/6); total (73/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01 | |||||||||||
Only 100 of 163 total aligned reads displayed.
TGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGGCAGGAAGG > W3110S.gb/4119833‑4119971 | gctgAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAg < 1:1161132/70‑1 (MQ=255) aaCAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTGTTAGATGt < 1:5784882/70‑1 (MQ=255) aaCAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATg < 1:3941294/69‑1 (MQ=255) aaCAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGtt < 1:6715431/71‑1 (MQ=255) aaCAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGtt > 1:7273056/1‑71 (MQ=255) aaCAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGtt > 1:5614435/1‑71 (MQ=255) aaCAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGtt > 1:1874807/1‑71 (MQ=255) aaCAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGtt > 1:1864862/1‑71 (MQ=255) aaCAGGAGAAGTTGAACCTGGGCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTGTTAGATGt < 1:4221674/70‑1 (MQ=255) cAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTTTCGTTACATCTAACAGATGTTcc > 1:3838553/1‑71 (MQ=255) cAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTc > 1:6606830/1‑70 (MQ=255) ggAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCt > 1:1028221/1‑71 (MQ=255) ggAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCt < 1:4253403/71‑1 (MQ=255) agaAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGtt > 1:2891174/1‑65 (MQ=255) aGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTGTTAGATGtaa > 1:6110285/1‑61 (MQ=38) aGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTGTTAGATGtaa > 1:397929/1‑61 (MQ=38) aGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTGTTAGATGtaa > 1:2260818/1‑61 (MQ=38) gTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGt > 1:5680683/1‑60 (MQ=255) ttGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCt < 1:6230181/64‑1 (MQ=255) ttGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCt < 1:884058/64‑1 (MQ=255) ttGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCa < 1:4918012/71‑1 (MQ=255) ttGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCa < 1:2603603/71‑1 (MQ=255) tGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAg > 1:7923536/1‑71 (MQ=255) gAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTcc < 1:833801/60‑1 (MQ=255) aaCCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAgg < 1:5215682/70‑1 (MQ=255) ccTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAg < 1:7860940/67‑1 (MQ=255) cTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGa < 1:3955327/50‑1 (MQ=255) cTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGa < 1:1276064/50‑1 (MQ=255) tGGTCGTGTCGCCAGACGTCAGCGGTACTGTGTCGTTACATCTAACAGa < 1:119330/49‑1 (MQ=255) tGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTGTTAGATGt < 1:7813468/52‑1 (MQ=37) tGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTGTTAGATGt < 1:4596050/52‑1 (MQ=37) tGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAgg > 1:1157202/1‑66 (MQ=255) tGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCa > 1:1923321/1‑68 (MQ=255) tCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACa > 1:5075195/1‑70 (MQ=255) tCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACa > 1:7491781/1‑70 (MQ=255) tgtCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCt < 1:2598720/51‑1 (MQ=255) tgtCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACt < 1:7566058/71‑1 (MQ=255) tCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGc < 1:6348585/55‑1 (MQ=255) tCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACa > 1:2759606/1‑65 (MQ=255) tCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACa > 1:4606978/1‑65 (MQ=255) gCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCAc < 1:5299571/59‑1 (MQ=255) gCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCAc < 1:4827381/59‑1 (MQ=255) gCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCAc < 1:6789828/59‑1 (MQ=255) gCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACaaa < 1:3730596/65‑1 (MQ=255) gCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTa < 1:1614352/70‑1 (MQ=255) cAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTg < 1:5089195/71‑1 (MQ=255) aGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTg > 1:6196928/1‑70 (MQ=255) gACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaa < 1:830633/71‑1 (MQ=255) gACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaa < 1:5783512/71‑1 (MQ=255) gACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaa < 1:5854511/71‑1 (MQ=255) gACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaa < 1:4401420/71‑1 (MQ=255) gACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaa < 1:4063159/71‑1 (MQ=255) gACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaa < 1:2530827/71‑1 (MQ=255) aCGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCa > 1:5904929/1‑53 (MQ=255) aCGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaaa < 1:6460168/71‑1 (MQ=255) cGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaaaa < 1:2362923/71‑1 (MQ=255) cGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaaaa < 1:26902/71‑1 (MQ=255) aGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTa < 1:2894625/51‑1 (MQ=255) aGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACaaa > 1:1434582/1‑55 (MQ=255) aGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACaaa > 1:6526558/1‑55 (MQ=255) aGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGt < 1:4065147/59‑1 (MQ=255) aGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGt > 1:2811964/1‑62 (MQ=255) aGCGCTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACaaa > 1:4167514/1‑55 (MQ=255) ggaCGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCCCTACAGTTTGTAGTgc > 1:2759731/3‑59 (MQ=255) gTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAGTTTGTAGTgc > 1:2045013/1‑59 (MQ=38) gTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGa > 1:5477323/1‑71 (MQ=255) tACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCa > 1:6546734/1‑43 (MQ=255) tACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCa > 1:3522496/1‑43 (MQ=255) tACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCa > 1:7884690/1‑43 (MQ=255) tACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGAc > 1:1677194/1‑71 (MQ=255) aCGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACt > 1:5560762/1‑71 (MQ=255) aCGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACt > 1:2204757/1‑71 (MQ=255) cGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCAc > 1:5125792/1‑42 (MQ=255) cGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaa < 1:2058481/58‑1 (MQ=255) ggTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGa > 1:1720993/1‑71 (MQ=255) tgtCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTg < 1:5326709/48‑1 (MQ=255) gtCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACt < 1:6823061/66‑1 (MQ=255) tCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACt < 1:5644004/45‑1 (MQ=255) cGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGa > 1:6986587/1‑66 (MQ=255) cGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGa > 1:7296561/1‑66 (MQ=255) gTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAAcgc > 1:7293633/1‑71 (MQ=255) gTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAAcg < 1:3702694/70‑1 (MQ=255) gTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAAcg < 1:2598065/70‑1 (MQ=255) ttACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTg < 1:1238199/43‑1 (MQ=255) ttACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTg < 1:7683829/43‑1 (MQ=255) ttACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTg < 1:3568588/43‑1 (MQ=255) aCATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCgg > 1:7548555/1‑70 (MQ=255) aCATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCgg > 1:7801026/1‑70 (MQ=255) aCATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCgg > 1:1615460/1‑70 (MQ=255) cATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaaaa > 1:1062207/1‑49 (MQ=255) cATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaaaa > 1:1901965/1‑49 (MQ=255) cATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGGCa < 1:7123507/71‑1 (MQ=255) aTCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGGCAg < 1:2610923/71‑1 (MQ=255) cTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGaaaa < 1:471168/46‑1 (MQ=255) cTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAg < 1:4261020/47‑1 (MQ=255) cTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGGCAgg > 1:4145598/1‑70 (MQ=255) cTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGGCAgg > 1:1394960/1‑70 (MQ=255) aaCAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGGc < 1:1381532/65‑1 (MQ=255) aCAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGGCAgg < 1:6306518/67‑1 (MQ=255) aCAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGGCAGGaa < 1:31473/69‑1 (MQ=255) | TGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGGCAGGAAGG > W3110S.gb/4119833‑4119971 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |