| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,165,966 | G→A | 5.7% | C34Y (TGT→TAT) | rpsL → | 30S ribosomal subunit protein S12 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,165,966 | 0 | G | A | 5.7% | 582.0 / 15.2 | 175 | C34Y (TGT→TAT) | rpsL | 30S ribosomal subunit protein S12 |
| Reads supporting (aligned to +/- strand): ref base G (75/90); new base A (5/5); total (80/95) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
Only 100 of 177 total aligned reads displayed.
CCACGTGCTCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCT > W3110S.gb/4165896‑4166035 | ccACGTGCTCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATg < 1:6888484/71‑1 (MQ=255) cTCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt < 1:7085720/71‑1 (MQ=255) tCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgtg > 1:2197301/1‑71 (MQ=255) cGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATATACACgag > 1:7668266/1‑68 (MQ=255) gCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGta > 1:2099612/1‑71 (MQ=255) gCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGta > 1:5827092/1‑71 (MQ=255) cAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGtat < 1:2919248/71‑1 (MQ=255) cAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGtat < 1:1030291/71‑1 (MQ=255) gTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtac < 1:6419565/71‑1 (MQ=255) ttGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtact < 1:18310/71‑1 (MQ=255) ttGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtact < 1:1978932/71‑1 (MQ=255) ttGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtact < 1:4440298/71‑1 (MQ=255) gAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtac < 1:3907716/66‑1 (MQ=255) aaaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGtata > 1:3704957/1‑61 (MQ=255) aaaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACt > 1:5386210/1‑71 (MQ=255) aaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgtgt > 1:5797082/1‑57 (MQ=255) aaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtac < 1:4915418/63‑1 (MQ=255) gCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATATACACGAGtacat < 1:1425664/59‑1 (MQ=37) cAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt < 1:2745789/52‑1 (MQ=255) cAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt < 1:4502735/52‑1 (MQ=255) cAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt < 1:7774705/52‑1 (MQ=255) cAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt < 1:7788889/52‑1 (MQ=255) aCGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTaa > 1:4717925/1‑70 (MQ=255) aCGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTaa > 1:6079271/1‑70 (MQ=255) cGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTaaaa < 1:3396519/71‑1 (MQ=255) gTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt < 1:6269075/48‑1 (MQ=255) gTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTaaaaa < 1:417023/71‑1 (MQ=255) gCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtacta > 1:6812811/1‑56 (MQ=255) gCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCa > 1:5449811/1‑59 (MQ=255) gCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCa > 1:7924128/1‑59 (MQ=255) ccTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAAcc > 1:2653683/1‑71 (MQ=255) cTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCg < 1:5284343/71‑1 (MQ=255) gcgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgtgt > 1:4228408/1‑44 (MQ=255) gcgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgtgt > 1:6480723/1‑44 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCg < 1:6652679/68‑1 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCg < 1:5084119/68‑1 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGaa < 1:1544636/70‑1 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGaa > 1:3240231/1‑70 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAAc < 1:1667475/71‑1 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAAc > 1:1996168/1‑71 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAAc < 1:7361638/71‑1 (MQ=255) gcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAAcc > 1:7015197/1‑66 (MQ=255) gcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACt > 1:4035675/1‑71 (MQ=255) gcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACt > 1:4219807/1‑71 (MQ=255) gcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTACTAAAAAACCGAACt > 1:1984956/1‑71 (MQ=255) cTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACt < 1:1091127/70‑1 (MQ=255) cTGGAAGCATGCCCGCAAAAACGTGGCGTATATACACgagt < 1:2800458/41‑1 (MQ=37) tGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTc < 1:2528514/36‑1 (MQ=255) tGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTc < 1:5778277/36‑1 (MQ=255) tGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTc < 1:5343855/36‑1 (MQ=255) tGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCt < 1:6500135/56‑1 (MQ=255) tGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCt < 1:875206/56‑1 (MQ=255) tGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTcc > 1:3743465/1‑71 (MQ=255) ggAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCcg > 1:7802142/1‑71 (MQ=255) gAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCg > 1:7757343/1‑63 (MQ=255) gAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAAcacg < 1:1113090/69‑4 (MQ=255) aaGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTa < 1:6615270/54‑1 (MQ=255) aGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtactac > 1:2623974/1‑45 (MQ=255) aGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtactac > 1:2527020/1‑45 (MQ=255) aGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACt > 1:4764968/1‑49 (MQ=255) aGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCcgcg > 1:5141936/1‑70 (MQ=255) aGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCgcgc > 1:1488801/1‑71 (MQ=255) gCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCt > 1:140965/1‑71 (MQ=255) gCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCt < 1:7753729/71‑1 (MQ=255) tGCCCGCAAAAACGTGGCGTATGTACTCGTGtata < 1:7690790/35‑1 (MQ=255) gCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGc > 1:7873005/1‑69 (MQ=255) cccGCAGAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTa < 1:492416/71‑1 (MQ=255) cccGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGt < 1:7504346/70‑1 (MQ=255) ccGCAAAAACGTGGCGTATGTACTCGTGTATAtact < 1:3969551/36‑1 (MQ=255) cGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTa > 1:7331110/1‑69 (MQ=255) gCAAAAACGTGGCGTATGTACTCGTGTATAtactac > 1:5388511/1‑36 (MQ=255) gCAAAAACGTGGCGTATGTACTCGTGTATAtacta > 1:6076105/1‑35 (MQ=255) gCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACt < 1:2344281/40‑1 (MQ=255) gCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACt < 1:4866687/40‑1 (MQ=255) cAAAAACGTGGCGTATGTACTCGTGTATATACTACCACt < 1:6728352/39‑1 (MQ=255) cAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAg > 1:1894253/1‑70 (MQ=255) cAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAg > 1:1656670/1‑70 (MQ=255) cAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAg > 1:2026376/1‑70 (MQ=255) cAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAg > 1:7111945/1‑70 (MQ=255) cAAAAACGTGGCGTATGTACTAGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGt < 1:4184770/71‑1 (MQ=255) aaaaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCgcgc < 1:4277843/60‑1 (MQ=255) aaaaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCgcgc < 1:3486668/60‑1 (MQ=255) aaaaaCGTGGCGTATGTACTCGTGTATATAATACCACTCCTAAAAAACCGAACTc > 1:6245973/1‑55 (MQ=255) aaaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTAt < 1:5843092/71‑1 (MQ=255) aaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTaaaa < 1:4988596/43‑1 (MQ=255) aaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATg > 1:5139954/1‑71 (MQ=255) aaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATg < 1:3587502/71‑1 (MQ=255) aCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGcc > 1:3117270/1‑71 (MQ=255) aCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGcc > 1:202232/1‑71 (MQ=255) cGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCt < 1:89830/56‑1 (MQ=255) cGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCt < 1:2227538/56‑1 (MQ=255) cGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGc > 1:4386817/1‑53 (MQ=255) cGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTc < 1:1657448/71‑1 (MQ=255) gTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTc > 1:7361175/1‑70 (MQ=255) tATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGt < 1:5536241/71‑1 (MQ=255) aTGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCgtgt > 1:1987114/1‑66 (MQ=255) tGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACt > 1:7156450/1‑40 (MQ=255) tGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGcc > 1:5299032/1‑61 (MQ=255) tGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGcc > 1:1162187/1‑61 (MQ=255) tGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTc > 1:2497640/1‑70 (MQ=255) | CCACGTGCTCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCT > W3110S.gb/4165896‑4166035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |