| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,165,970 | T→A | 5.5% | T35T (ACT→ACA) | rpsL → | 30S ribosomal subunit protein S12 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,165,970 | 0 | T | A | 5.5% | 515.4 / 17.0 | 180 | T35T (ACT→ACA) | rpsL | 30S ribosomal subunit protein S12 |
| Reads supporting (aligned to +/- strand): ref base T (79/91); new base A (5/5); total (84/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
Only 100 of 184 total aligned reads displayed.
TGCTCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACT > W3110S.gb/4165901‑4166039 | gCTCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCg > 1:7006714/1‑71 (MQ=255) cTCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt < 1:7356481/71‑1 (MQ=255) cTCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt > 1:4908565/1‑71 (MQ=255) cTCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt < 1:7085720/71‑1 (MQ=255) tCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgtg > 1:2197301/1‑71 (MQ=255) cGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATATACACgag > 1:4773619/1‑68 (MQ=255) gCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGta > 1:5827092/1‑71 (MQ=255) gCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGta > 1:2099612/1‑71 (MQ=255) cAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGtat < 1:1030291/71‑1 (MQ=255) cAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGtat < 1:7234/71‑1 (MQ=255) aaaGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGtata > 1:5277315/1‑71 (MQ=255) ttGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtact < 1:18310/71‑1 (MQ=255) ttGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtact < 1:4440298/71‑1 (MQ=255) gAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTTGCGTATGTACTCgt < 1:753389/58‑1 (MQ=255) gAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtac < 1:3917039/66‑1 (MQ=255) aaaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGtata > 1:2412695/1‑61 (MQ=255) aaaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGtat > 1:3713928/1‑60 (MQ=255) aaaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACt > 1:5386210/1‑71 (MQ=255) aaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgtgt > 1:5797082/1‑57 (MQ=255) aaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCg > 1:6716249/1‑54 (MQ=255) aaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtac < 1:4702933/63‑1 (MQ=255) aaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtac < 1:5777673/63‑1 (MQ=255) aaGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATATACACGAGTACAtac > 1:6630367/1‑63 (MQ=37) aGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTcc > 1:768251/1‑71 (MQ=255) gCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATATACACGAGtacat < 1:1425664/59‑1 (MQ=37) cAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt < 1:7774705/52‑1 (MQ=255) aCGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTaa > 1:6079271/1‑70 (MQ=255) cGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTaaaa < 1:261475/71‑1 (MQ=255) cGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTaaaa < 1:3396519/71‑1 (MQ=255) gTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgt < 1:6269075/48‑1 (MQ=255) gTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTaaaaa < 1:417023/71‑1 (MQ=255) gCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtacta > 1:6812811/1‑56 (MQ=255) gCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCa > 1:5449811/1‑59 (MQ=255) gCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCa > 1:7924128/1‑59 (MQ=255) gCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTaaaaaa < 1:5303238/70‑1 (MQ=255) ccTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAAcc > 1:2527607/1‑71 (MQ=255) tGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCAc < 1:77936/57‑1 (MQ=255) tGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCg < 1:5287973/70‑1 (MQ=255) gcgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgtgt > 1:6480723/1‑44 (MQ=255) gcgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCgtgt > 1:4228408/1‑44 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGaa > 1:3240231/1‑70 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAAc < 1:7361638/71‑1 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAAc > 1:1996168/1‑71 (MQ=255) cgcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAAc < 1:1667475/71‑1 (MQ=255) gcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAAcc > 1:7015197/1‑66 (MQ=255) gcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACt > 1:4035675/1‑71 (MQ=255) gcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACt > 1:4219807/1‑71 (MQ=255) gcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACt < 1:2762054/71‑1 (MQ=255) gcTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTACTAAAAAACCGAACt > 1:1984956/1‑71 (MQ=255) cTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACt < 1:1091127/70‑1 (MQ=255) cTGGAAGCATGCCCGCAAAAACGTGGCGTATATACACgagt < 1:1933935/41‑1 (MQ=37) cTGGAAGCATGCCCGCAAAAACGTGGCGTATATACACgagt < 1:361121/41‑1 (MQ=37) tGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCt < 1:6500135/56‑1 (MQ=255) tGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCt < 1:875206/56‑1 (MQ=255) gAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGta < 1:3814217/39‑1 (MQ=255) gAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCg > 1:7757343/1‑63 (MQ=255) gAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAAcacg < 1:1113090/69‑4 (MQ=255) aaGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTa < 1:6615270/54‑1 (MQ=255) aaGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAAc < 1:1494498/60‑1 (MQ=255) aGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATAtactac > 1:2623974/1‑45 (MQ=255) aGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACt > 1:4764968/1‑49 (MQ=255) aGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCcgcg > 1:5141936/1‑70 (MQ=255) aGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCgcgc > 1:1488801/1‑71 (MQ=255) gCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCg < 1:251092/60‑1 (MQ=255) tGCCCGCAAAAACGTGGCGTATGTACTCGTGtata < 1:7699807/35‑1 (MQ=255) gCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGc > 1:7873005/1‑69 (MQ=255) gCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCg > 1:2809676/1‑70 (MQ=255) cccGCAGAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTa < 1:492416/71‑1 (MQ=255) cccGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTaaaaaa > 1:5104206/1‑52 (MQ=255) ccGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTaa < 1:2659589/71‑1 (MQ=255) ccGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTa > 1:5472065/1‑70 (MQ=255) gCAAAAACGTGGCGTATGTACTCGTGTATAtactac > 1:5388511/1‑36 (MQ=255) gCAAAAACGTGGCGTATGTACTCGTGTATAtacta > 1:6076105/1‑35 (MQ=255) cAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAg > 1:1894253/1‑70 (MQ=255) cAAAAACGTGGCGTATGTACTAGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGt < 1:4184770/71‑1 (MQ=255) aaaaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCgcgc < 1:4277843/60‑1 (MQ=255) aaaaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCgcgc < 1:3486668/60‑1 (MQ=255) aaaaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTa > 1:3306386/1‑71 (MQ=255) aaaaaCGTGGCGTATGTACTCGTGTATATAATACCACTCCTAAAAAACCGAACTc > 1:6245973/1‑55 (MQ=255) aaaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTAt < 1:5843092/71‑1 (MQ=255) aaaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTAt < 1:7779274/71‑1 (MQ=255) aaaCGTGGCGTATGTACTCGTGTATATAGTACCACTCCTaaaaa < 1:7130221/44‑1 (MQ=255) aaaCGTGGCGTATGTACTCGTGTATATACTACCACTCCTaaaa < 1:4988596/43‑1 (MQ=255) aCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGcc > 1:3117270/1‑71 (MQ=255) aCGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGcc > 1:202232/1‑71 (MQ=255) cGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCt < 1:89830/56‑1 (MQ=255) cGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCt < 1:2227538/56‑1 (MQ=255) gTGGCGTATGTACTCGTGTATATACTACCACTCCTaaaa < 1:4721659/39‑1 (MQ=255) tagTATATACACGAGTACATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTa < 1:747112/60‑1 (MQ=25) cGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGc > 1:4386817/1‑53 (MQ=255) cGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTc < 1:1657448/71‑1 (MQ=255) aTGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCgtgt > 1:1987114/1‑66 (MQ=255) aTGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTc > 1:1553047/1‑71 (MQ=255) tGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGcc > 1:1162187/1‑61 (MQ=255) tGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCt < 1:2060190/71‑1 (MQ=255) aCTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGc > 1:6735771/1‑57 (MQ=255) cTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTaa > 1:3807858/1‑49 (MQ=255) cTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACt < 1:1276341/71‑1 (MQ=255) tCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACt > 1:7041087/1‑70 (MQ=255) tCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACt > 1:6338513/1‑70 (MQ=255) | TGCTCGCAAAGTTGCGAAAAGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTGTATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACT > W3110S.gb/4165901‑4166039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |