| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,273,734 | G→A | 7.3% | intergenic (+15/‑96) | aphA → / → yjbQ | acid phosphatase/phosphotransferase, class B, non‑specific/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,273,734 | 0 | G | A | 7.3% | 214.2 / 5.4 | 68 | intergenic (+15/‑96) | aphA/yjbQ | acid phosphatase/phosphotransferase, class B, non‑specific/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (34/29); new base A (3/2); total (37/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01 | |||||||||||
CCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAT > W3110S.gb/4273664‑4273802 | ccTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCg < 1:7397882/71‑1 (MQ=255)ccTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCg < 1:1089344/71‑1 (MQ=255)ccTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCg < 1:6488976/71‑1 (MQ=255) cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGt < 1:4652089/71‑1 (MQ=255) tGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGa > 1:974744/1‑71 (MQ=255) gCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTg < 1:1169190/69‑1 (MQ=255) gCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGAt > 1:1012264/1‑71 (MQ=255) caAGCGTGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGctc > 1:7273453/1‑71 (MQ=255) caAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGctc > 1:5630849/1‑71 (MQ=255) caAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGctc > 1:5315665/1‑71 (MQ=255) caAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGctc > 1:5275783/1‑71 (MQ=255) caAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGctc > 1:4370238/1‑71 (MQ=255) aaGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATg > 1:2509903/1‑67 (MQ=255) aaGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGctct < 1:3376027/71‑1 (MQ=255) aaGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGctct < 1:2342196/71‑1 (MQ=255) gCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGa > 1:7234337/1‑63 (MQ=255) gggTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCg < 1:6268709/71‑1 (MQ=255) gggTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCg < 1:3224419/71‑1 (MQ=255) ggTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCg < 1:3215250/70‑1 (MQ=255) ggTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGa < 1:3611735/71‑1 (MQ=255) gTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGt < 1:4427304/57‑1 (MQ=255) gTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGt < 1:4759210/57‑1 (MQ=255) gTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATGATGCTCTCCCGaa < 1:5242993/71‑1 (MQ=255) cGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGc > 1:2968568/1‑71 (MQ=255) gTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCt > 1:5107854/1‑71 (MQ=255) tttGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCt < 1:3183617/70‑1 (MQ=255) agagGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGttttttt < 1:2503720/70‑1 (MQ=255) gagGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTcc > 1:6984305/1‑53 (MQ=255) gagGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTcc > 1:1081376/1‑53 (MQ=255) gagGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTcc > 1:2078344/1‑53 (MQ=255) gTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGt < 1:1187125/40‑1 (MQ=255) gTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGt < 1:1644037/40‑1 (MQ=255) gTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGt < 1:4878263/40‑1 (MQ=255) gTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGt < 1:4251263/40‑1 (MQ=255) gTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAAta > 1:3869364/1‑69 (MQ=255) gTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATca > 1:4341210/1‑71 (MQ=255) aTCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTg > 1:7475515/1‑38 (MQ=255) aTCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTCTTAATcagtt < 1:4909571/71‑4 (MQ=255) gTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGtttttt < 1:809715/59‑1 (MQ=255) tCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCttt < 1:4011850/71‑1 (MQ=255) cAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGttt < 1:2894169/54‑1 (MQ=255) cAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTa > 1:2478256/1‑71 (MQ=255) cAATTCAGAATACTGACAGAGCGGGAGAGCATCACGCTCTccc > 1:381165/1‑43 (MQ=21) tCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTccc > 1:6401643/1‑39 (MQ=255) cAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAAt > 1:177871/1‑56 (MQ=255) cAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCtt > 1:7734626/1‑71 (MQ=255) aGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCttt < 1:4022454/71‑1 (MQ=255) aGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCtt < 1:4135758/70‑1 (MQ=255) aGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCtt < 1:6754993/70‑1 (MQ=255) gAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTc > 1:1053500/1‑71 (MQ=255) aaTACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCg > 1:7574915/1‑71 (MQ=255) aCAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCtt > 1:5052712/1‑71 (MQ=255) aCAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt < 1:6357582/70‑1 (MQ=255) aCAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt < 1:7527913/70‑1 (MQ=255) aCAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt < 1:2957550/70‑1 (MQ=255) cAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCg > 1:954632/1‑63 (MQ=255) cAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtg < 1:6929056/71‑1 (MQ=255) agagCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACAcc > 1:7555007/1‑50 (MQ=255) agagCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgc > 1:91390/1‑71 (MQ=255) agagCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgc > 1:7163036/1‑71 (MQ=255) agCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgctg > 1:4520437/1‑71 (MQ=255) cGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgctg > 1:1871767/1‑69 (MQ=255) gagCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCacac > 1:3474469/1‑40 (MQ=255) gagCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCacac > 1:6547985/1‑40 (MQ=255) gagCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACt > 1:3979077/1‑70 (MQ=255) gagCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACt > 1:3609283/1‑70 (MQ=255) agCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGa < 1:3244398/71‑1 (MQ=255) cGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAt > 1:7282971/1‑70 (MQ=255) cGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAt < 1:1159188/70‑1 (MQ=255) cGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAt > 1:3192568/1‑70 (MQ=255) cGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAt < 1:3196488/70‑1 (MQ=255) gTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCTCTGTCTTGCTGCAAACt < 1:6149732/66‑1 (MQ=255) | CCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAT > W3110S.gb/4273664‑4273802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |