| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,285,507 | A→T | 10.5% | S387R (AGT→AGA) ‡ | yjcF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,285,507 | 0 | A | T | 10.5% | 309.4 / 25.2 | 116 | S387R (AGT→AGA) ‡ | yjcF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (47/56); new base T (7/5); total (54/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
AGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGA > W3110S.gb/4285438‑4285577 | aGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCAc > 1:14519/1‑71 (MQ=255) gTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCAc < 1:4349142/70‑1 (MQ=255) ttGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTAt > 1:2815005/1‑71 (MQ=255) aCATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATg > 1:56096/1‑71 (MQ=255) tGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGgag < 1:2746764/71‑1 (MQ=255) tGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGgag < 1:6681249/71‑1 (MQ=255) ccAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCTCCATTaa < 1:6169822/64‑1 (MQ=255) cAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGata > 1:6558834/1‑71 (MQ=255) cAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGata > 1:2329128/1‑71 (MQ=255) cAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGata < 1:4351503/71‑1 (MQ=255) cAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGata > 1:3179428/1‑71 (MQ=255) cAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTCTAGTGTTACCAGGATATCACTATTAATGGAGata > 1:7610370/1‑71 (MQ=255) aGTATGAGTTGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCTCCATTAAta < 1:5661808/64‑2 (MQ=255) aGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCTCCATTAAta < 1:4331607/64‑2 (MQ=255) aGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATg > 1:606329/1‑64 (MQ=255) gTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCAc < 1:7609264/55‑1 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCTCCATTAAtagt > 1:5699654/1‑61 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCTCCATTAAtagt > 1:5013465/1‑61 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCTCCATTAAtagt > 1:5827131/1‑61 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTAtt < 1:7114475/58‑1 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTAtt < 1:7574800/58‑1 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTAtt < 1:7141638/58‑1 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATg < 1:1350562/62‑1 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGATATAttt < 1:16043/71‑1 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATAttt < 1:6369810/71‑1 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATAttt < 1:4921141/71‑1 (MQ=255) tATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATAttt < 1:1122661/71‑1 (MQ=255) aTGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATAtttt > 1:6561846/1‑71 (MQ=255) tGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCTCCATTAATAGTGatatcct > 1:1599006/1‑67 (MQ=25) tGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTc > 1:7109179/1‑71 (MQ=255) tGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTc > 1:1067910/1‑71 (MQ=255) tGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTc > 1:6953685/1‑71 (MQ=255) tGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTc < 1:6294341/71‑1 (MQ=255) aGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCCCTATTaa < 1:3440635/56‑1 (MQ=255) aGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTaa < 1:4226715/56‑1 (MQ=255) aGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTaa < 1:1057043/56‑1 (MQ=255) tGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTAtt < 1:363986/52‑1 (MQ=255) tGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATg > 1:277535/1‑56 (MQ=255) tGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTcc < 1:2837621/68‑1 (MQ=255) gCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTcac > 1:1262516/1‑70 (MQ=255) cacaATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCTCCATTAAtag > 1:6413945/1‑52 (MQ=38) acaATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTcaccac < 1:4236146/71‑1 (MQ=255) acaATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTcaccac < 1:7693754/71‑1 (MQ=255) caATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACg > 1:2707330/1‑71 (MQ=255) caATCTTCTTCAAAGAATTTCTGTAGTATTACCAGGATATCACTATTaa > 1:5554179/1‑49 (MQ=255) aaTCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTc > 1:718016/1‑61 (MQ=255) aTCTCCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACg > 1:2061019/1‑69 (MQ=255) tcttctTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACg > 1:4234496/1‑68 (MQ=255) cttcttCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGat < 1:4494936/52‑1 (MQ=255) cttcttCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGat < 1:7301526/52‑1 (MQ=255) ttcttcAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGaa > 1:4829980/1‑71 (MQ=255) ttcttcAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGaa > 1:1674843/1‑71 (MQ=255) tcAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATAtt < 1:3916003/50‑1 (MQ=255) cAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATg < 1:1214963/68‑1 (MQ=255) aaaGAATTTCTGCAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTaa > 1:7070773/1‑71 (MQ=255) aaGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATg > 1:6624332/1‑66 (MQ=255) aGAATTTCTGTAGTGTTACCAGGATATCTCCATTAATAGTGatat < 1:7921279/45‑1 (MQ=18) aGAATTTCTGTAGTGTTACCAGGATATCTCCATTAATAGTGatat < 1:7391900/45‑1 (MQ=18) aaTTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATAcc < 1:668430/71‑1 (MQ=255) aTTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATAcc > 1:7142870/1‑70 (MQ=255) cTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTc > 1:4352828/1‑71 (MQ=255) gTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTcac < 1:5866781/45‑1 (MQ=255) tAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCt > 1:4103437/1‑71 (MQ=255) tAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCt > 1:2195630/1‑71 (MQ=255) aGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTcacca > 1:2681847/1‑45 (MQ=255) aGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTcacca > 1:7135051/1‑45 (MQ=255) gtgtTACCAGGATATCACTATTAATGGAGGTATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTg < 1:3564977/70‑1 (MQ=255) gtgtTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTg < 1:6889764/70‑1 (MQ=255) gtgtTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTg > 1:4894910/1‑70 (MQ=255) ttttACCAGGATATCACTATTAATGGAGATATTTTCCTcacca < 1:6379262/41‑1 (MQ=255) gtTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGAc > 1:1867847/1‑71 (MQ=255) ttACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCa < 1:868079/60‑1 (MQ=255) ttACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCa < 1:4532046/60‑1 (MQ=255) ttACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCa < 1:4785981/60‑1 (MQ=255) ttACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACg < 1:6757487/71‑1 (MQ=255) tACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGc > 1:5844083/1‑71 (MQ=255) aCCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATAccc < 1:3851622/57‑1 (MQ=255) aCCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTg > 1:7911982/1‑65 (MQ=255) ccAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTa < 1:6361016/71‑1 (MQ=255) ccAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTa < 1:6857110/71‑1 (MQ=255) cAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAg < 1:7517571/71‑1 (MQ=255) cAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAg < 1:3549779/71‑1 (MQ=255) cAGGATATCACTATTAATGGAGATATTTTCCTAACCACGGAGAATGGTAATACCCATTCCCt > 1:3672195/1‑62 (MQ=255) aGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGaa > 1:3384170/1‑43 (MQ=255) aGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGAc < 1:3150919/65‑1 (MQ=255) aGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGAc < 1:6919827/65‑1 (MQ=255) aGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACACATTCCCTGGACGCt > 1:485478/1‑68 (MQ=255) gATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCt < 1:7715919/71‑1 (MQ=255) atatCTCCATTAATAGTGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCt > 1:7620196/1‑70 (MQ=38) atatCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCt > 1:5434930/1‑70 (MQ=255) atatCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTc < 1:6000118/71‑1 (MQ=255) atatCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCATGGACGCTAGCCTc < 1:7168078/71‑1 (MQ=255) tatCACTATTAATGGAGATATTTTCCTCACCACGGAGAATgg < 1:5488762/42‑1 (MQ=255) tatCACTATTAATGGAGATATTTTCCTCACCACGGAGAATgg < 1:2183154/42‑1 (MQ=255) tatCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCa > 1:5946545/1‑51 (MQ=255) atCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTaa > 1:5476717/1‑44 (MQ=255) tCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTa > 1:3342691/1‑42 (MQ=255) cACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATa > 1:4875473/1‑44 (MQ=255) actactAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGa < 1:747283/71‑1 (MQ=255) aCTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCg > 1:2592368/1‑70 (MQ=255) | AGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGA > W3110S.gb/4285438‑4285577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |