BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	218,391	G→A	5.4%	P129S (CCG→TCG)	proS ←	prolyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	218,391	0	G	A	5.4%	359.0 / 6.8	111	P129S (CCG→TCG)	proS	prolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (56/47); new base A (3/3); total (59/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 119 total aligned reads displayed.

CGCATGACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAG  >  W3110S.gb/218324‑218460

cGCATGACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAg                                                                    >  1:4019046/1‑71 (MQ=255)

cGCATGACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAg                                                                    >  1:3024779/1‑71 (MQ=255)

     gACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGtt                                                               >  1:692820/1‑71 (MQ=255)

     gACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGtt                                                               >  1:5690573/1‑71 (MQ=255)

     gACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGt                                                                >  1:6040030/1‑70 (MQ=255)

       cGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTg                                                             >  1:6008168/1‑71 (MQ=255)

       cGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTg                                                             >  1:38012/1‑71 (MQ=255)

        gCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGt                                                            <  1:5154705/71‑1 (MQ=255)

         ccGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGt                                                            >  1:2217620/1‑70 (MQ=255)

         ccGAAACGCGGACGCACTTCGTCGAGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGt                                                            >  1:5081512/1‑70 (MQ=255)

          cGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCATCTGTTTGTaa                                                          <  1:7920691/71‑1 (MQ=255)

          cGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTaa                                                          <  1:5016081/71‑1 (MQ=255)

            aaaCGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTaa                                                          >  1:582428/1‑69 (MQ=255)

            aaaCGAGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTaa                                                          >  1:107697/1‑69 (MQ=255)

               cgcgGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAg                                                                    >  1:3390775/1‑56 (MQ=255)

                gcgGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGt                                                                <  1:928186/59‑1 (MQ=255)

                gcgGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAag                                                         <  1:6857852/66‑1 (MQ=255)

                   gACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCt                                                     <  1:6419768/67‑1 (MQ=255)

                     cGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTc                                               <  1:6434358/71‑1 (MQ=255)

                      gCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTc                                               >  1:2860205/1‑70 (MQ=255)

                      gCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCg                                              >  1:1414550/1‑71 (MQ=255)

                      gCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCg                                              >  1:5851033/1‑71 (MQ=255)

                        aCTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGt                                             >  1:6964618/1‑70 (MQ=255)

                         cTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTa                                           >  1:2592613/1‑71 (MQ=255)

                           tcgtcgCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAagag                                                       >  1:4461647/1‑57 (MQ=255)

                           tcgtcgCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAg                                                  >  1:6757919/1‑62 (MQ=255)

                           tcgtcgCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTAc                                          >  1:4116563/1‑70 (MQ=255)

                           tcgtcgCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTAc                                          >  1:4125926/1‑70 (MQ=255)

                            cgtcgCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGt                                             >  1:7905706/1‑66 (MQ=255)

                            cgtcgCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACg                                         >  1:3223692/1‑70 (MQ=255)

                             gtcgCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGaa                                       <  1:3680193/71‑1 (MQ=255)

                              tcgCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAAt                                      <  1:1666405/71‑1 (MQ=255)

                                gcgGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGa                                        <  1:3086800/67‑1 (MQ=255)

                                gcgGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGa                                        <  1:2072540/67‑1 (MQ=255)

                                  ggAACTTGGTCTGTATCTGATAGAAGTTGATCGGCAGTTGCTTGTAGCTGTTCAGCTCGTTGCgcgc                                      <  1:2288271/67‑4 (MQ=255)

                                  ggAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCt                                                                  <  1:5537175/39‑1 (MQ=255)

                                  ggAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAAt                                      >  1:3314243/1‑67 (MQ=255)

                                   gAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGt                                                            <  1:334849/44‑1 (MQ=255)

                                    aaCTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGt                                                            >  1:6680076/1‑43 (MQ=255)

                                    aaCTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGt                                                            >  1:6294556/1‑43 (MQ=255)

                                    aaCTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAagag                                                       >  1:4133012/1‑48 (MQ=255)

                                    aaCTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAagag                                                       >  1:1418989/1‑48 (MQ=255)

                                    aaCTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAagag                                                       >  1:7208817/1‑48 (MQ=255)

                                    aaCTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAAt                                      >  1:6696357/1‑65 (MQ=255)

                                    aaCTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAAt                                      >  1:3293076/1‑65 (MQ=255)

                                     aCTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGt                                 >  1:1999299/1‑69 (MQ=255)

                                     aCTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCa                               <  1:6410613/71‑1 (MQ=255)

                                        tGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTaa                                                          >  1:3849387/1‑41 (MQ=255)

                                        tGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTa                                                           >  1:4823084/1‑40 (MQ=255)

                                        tGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCa                               >  1:5755936/1‑68 (MQ=255)

                                        tGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGt                             >  1:2058648/1‑70 (MQ=255)

                                          gTCTGGATCTGATAGAAGTTCAGCGTCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTGAGTGAt                          <  1:10405/71‑1 (MQ=255)

                                             tGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGAGATAAc                       >  1:4335129/1‑71 (MQ=255)

                                               gATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAAt                                      <  1:3296964/54‑1 (MQ=255)

                                               gATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAAct                      <  1:1517594/70‑1 (MQ=255)

                                                aTCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAActtc                    >  1:6110765/1‑71 (MQ=255)

                                                 tCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTa                                                    <  1:5261824/38‑1 (MQ=255)

                                                    gATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATaa                        >  1:3385821/1‑63 (MQ=255)

                                                    gATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATaa                        >  1:2785976/1‑63 (MQ=255)

                                                    gATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATaa                        >  1:831206/1‑63 (MQ=255)

                                                    gATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCa                >  1:6147475/1‑71 (MQ=255)

                                                     aTAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAActtct                   <  1:4666057/67‑1 (MQ=255)

                                                      tAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATg              <  1:5190106/71‑1 (MQ=255)

                                                        gAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGa             <  1:1898471/70‑1 (MQ=255)

                                                        gAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGa             <  1:6085443/70‑1 (MQ=255)

                                                        caaGTTCCGCGACAGCTGTTTGTAAGAGCTAAGCTCGTTACGa                                        <  1:1596678/42‑1 (MQ=255)

                                                        caaGTTCCGCGACAGCTGTTTGTAAGAGCTAAGCTCGTTACGa                                        <  1:710519/42‑1 (MQ=255)

                                                        caaGTTCCGCGACAGCTGTTTGTAAGAGCTAAGCTCGTTACGa                                        <  1:2662424/42‑1 (MQ=255)

                                                         aaGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCTTTACGAATCAGGTCAGTGATAACTTCTTCATGAGt           <  1:4451670/71‑1 (MQ=255)

                                                          aGTTCCGCGACAGCTGTTTGTAAGAGCTAAGCTCGt                                             >  1:2747630/1‑36 (MQ=255)

                                                          aGTTCCGCGACAGCTGTTTGTAAGAGCTAAGCTCGTTACGAAt                                      >  1:3379736/1‑43 (MQ=255)

                                                          aGTTCCGCGACAGCTGTTTGTAAGAGCTAAGCTCGTTACGAAt                                      >  1:27411/1‑43 (MQ=255)

                                                          aGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCATTGATAACTTCTTCATGa             <  1:351332/68‑1 (MQ=255)

                                                          aGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGtt          <  1:5371099/71‑1 (MQ=255)

                                                          aGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGtt          <  1:1182500/71‑1 (MQ=255)

                                                          aGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGtt          <  1:7677700/71‑1 (MQ=255)

                                                           gTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTa                                           >  1:1905171/1‑37 (MQ=255)

                                                           gTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTa                                           >  1:7559851/1‑37 (MQ=255)

                                                           gTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTa                                           >  1:4080378/1‑37 (MQ=255)

                                                           gTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTAc                                          <  1:7515506/38‑1 (MQ=255)

                                                           gTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTAc                                          <  1:5058852/38‑1 (MQ=255)

                                                           gTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTg         <  1:7725902/71‑1 (MQ=255)

                                                           gTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTg         >  1:3021830/1‑71 (MQ=255)

                                                           gTTCAGAGGCAGCTGTTTGTAAGAGCTAAGCTCGTTa                                           >  1:6526426/1‑37 (MQ=255)

                                                            ttCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACg                                         <  1:1786252/38‑1 (MQ=255)

                                                            ttCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACg                                         <  1:5368019/38‑1 (MQ=255)

                                                            ttCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACg                                         <  1:1608344/38‑1 (MQ=255)

                                                             tCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTggg       >  1:5322607/1‑71 (MQ=255)

                                                               aGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGcc     <  1:3705983/71‑1 (MQ=255)

                                                                gCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCg    <  1:6740913/71‑1 (MQ=255)

                                                                 cGGCAGCTTTTTCTAAGAGCTAAGCTCGTTACGAATCAg                                   <  1:6448967/39‑1 (MQ=37)

                                                                 cGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGa   <  1:6127031/71‑1 (MQ=255)

                                                                  ggCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAg  <  1:6628943/71‑1 (MQ=255)

                                                                  ggCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAg  <  1:3594739/71‑1 (MQ=255)

                                                                  ggCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAg  <  1:4063747/71‑1 (MQ=255)

                                                                  ggCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAg  <  1:5463880/71‑1 (MQ=255)

                                                                  ggCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAg  <  1:1171128/71‑1 (MQ=255)

                                                                   gCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACttcttc                 <  1:7776145/55‑1 (MQ=255)

                                                                   gCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACttcttc                 <  1:7871739/55‑1 (MQ=255)

                                                                   gCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAg  <  1:3891337/70‑1 (MQ=255)

CGCATGACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTGGATCTGATAGAAGTTCAGCGGCAGCTGTTTGTAAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAG  >  W3110S.gb/218324‑218460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`