Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,425,615 C→A 8.4% I318I (ATC→ATA ulaA → L‑ascorbate‑specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,425,6150CA 8.4% 261.5 / 5.2 81I318I (ATC→ATAulaAL‑ascorbate‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base C (36/38);  new base A (3/4);  total (39/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCATTTAACGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGT  >  W3110S.gb/4425548‑4425683
                                                                   |                                                                    
aaGCATTTAACGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGctatc                                                                      <  1:7002951/68‑1 (MQ=255)
     tttAACGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCtt                                                              <  1:7663551/71‑1 (MQ=255)
      ttAACGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATAGATAGCt                                                               <  1:350922/69‑1 (MQ=255)
       tAACGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTcg                                                            >  1:7162719/1‑71 (MQ=255)
       tAACGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTcg                                                            >  1:649665/1‑71 (MQ=255)
        aaCGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTcgc                                                           >  1:4558177/1‑71 (MQ=255)
        aaCGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTcgc                                                           >  1:5879128/1‑71 (MQ=255)
        aaCGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGAAGCTATAGATAGCtgca                                                            <  1:3568344/70‑4 (MQ=255)
         aCGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTcgcg                                                          <  1:6380392/71‑1 (MQ=255)
          cGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCgcgc                                                         <  1:1792639/71‑1 (MQ=255)
           ggCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGcc                                                        >  1:1110113/1‑71 (MQ=255)
           ggCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTCTGCAGCTATCTATAGCTTCGCGcc                                                        >  1:3568266/1‑71 (MQ=255)
            gCATTTCCCAGCTCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCg                                                       <  1:3400638/71‑1 (MQ=255)
            gCATTTCCCAGCTCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCg                                                       <  1:4086697/71‑1 (MQ=255)
            gCATTTCCCAGCTCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCg                                                       <  1:5694322/71‑1 (MQ=255)
            gCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGcc                                                        >  1:5863915/1‑70 (MQ=255)
             cATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAg                                                                 >  1:6258098/1‑60 (MQ=255)
              aTTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGctatctat                                                                   <  1:1730966/57‑1 (MQ=255)
              aTTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGaa                                                     >  1:6134262/1‑71 (MQ=255)
              aTTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGaa                                                     >  1:4087780/1‑71 (MQ=255)
              aTTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGaa                                                     >  1:1080782/1‑71 (MQ=255)
                 tCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGctatctat                                                                   >  1:3129765/1‑54 (MQ=255)
                   ccAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCg                                                <  1:3617164/71‑1 (MQ=255)
                   ccAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCg                                                >  1:822910/1‑71 (MQ=255)
                     aGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTg                                              >  1:5503104/1‑71 (MQ=255)
                      gcgcCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCt                                                               <  1:1040046/53‑1 (MQ=255)
                      gcgcCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCt                                                               <  1:4836661/53‑1 (MQ=255)
                      gcgcCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCt                                                               <  1:3181961/53‑1 (MQ=255)
                         ccTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTc                                                             >  1:6783612/1‑52 (MQ=255)
                          cTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGctatc                                                                      >  1:5398221/1‑42 (MQ=255)
                          cTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAg                                                                 <  1:3426663/47‑1 (MQ=255)
                          cTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTg                                         <  1:7035802/71‑1 (MQ=255)
                          cTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTg                                         <  1:2056126/71‑1 (MQ=255)
                          cTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGAAGCTATAGATAGCt                                                               <  1:4557540/49‑1 (MQ=38)
                           tGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTgg                                        >  1:2196391/1‑71 (MQ=255)
                           tGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTgg                                        >  1:5050184/1‑71 (MQ=255)
                           tGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTg                                         <  1:6005082/70‑1 (MQ=255)
                           tGATCCCAGGTGCGGTTCTGGCGATTGACTGTGAAGCTATAGATAGCt                                                               <  1:7455082/48‑1 (MQ=37)
                            gATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTggg                                       >  1:6175767/1‑71 (MQ=255)
                            gATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTggg                                       >  1:6621786/1‑71 (MQ=255)
                            gATCCCAGGTGCGGTTCTGGCGATTGACTGTGAAGCTATAGATAGCtgc                                                             >  1:4081772/1‑47 (MQ=25)
                            gATCCCAGGTGCGGTTCTGGCGATTGACTGTGAAGCTATAGATAGCtgc                                                             >  1:2079242/1‑47 (MQ=25)
                            gATCCCAGGTGCGGTTCTGGCGATTGACTGTGAAGCTATAGATAGCtgc                                                             >  1:3019080/1‑47 (MQ=25)
                                 cAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCtt                                   >  1:4225652/1‑70 (MQ=255)
                                  aGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGa                                                      >  1:5614367/1‑50 (MQ=255)
                                  aGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGa                                                      >  1:1496226/1‑50 (MQ=255)
                                  aGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTa                                 <  1:3221157/71‑1 (MQ=255)
                                  aGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTa                                 <  1:5360988/71‑1 (MQ=255)
                                   ggTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTAt                                <  1:2710528/71‑1 (MQ=255)
                                    gTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTAtg                               >  1:2626025/1‑71 (MQ=255)
                                     tGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTAtgt                              <  1:2535205/71‑1 (MQ=255)
                                       cGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTgg                            <  1:5143017/71‑1 (MQ=255)
                                       cGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTgg                            <  1:6404292/71‑1 (MQ=255)
                                        ggTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTgg                            >  1:3660727/1‑70 (MQ=255)
                                        ggTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTgg                            >  1:7378883/1‑70 (MQ=255)
                                         gTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGACCGCCGTGGTCTGGGGCTTTATGTgggg                          >  1:2976120/1‑71 (MQ=255)
                                         gTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTgggg                          <  1:4973347/71‑1 (MQ=255)
                                         gTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTgggg                          <  1:2278032/71‑1 (MQ=255)
                                         gTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGCTTTATGTGGGGc                         <  1:293702/71‑1 (MQ=255)
                                          ttCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTa                                 <  1:3563149/63‑1 (MQ=255)
                                          ttCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTggg                           <  1:2468398/69‑1 (MQ=255)
                                          ttCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTggg                           <  1:7258313/69‑1 (MQ=255)
                                            cTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGc                                                  <  1:5865005/44‑1 (MQ=255)
                                            cTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTAtg                               >  1:6067029/1‑63 (MQ=255)
                                            cTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCAc                       >  1:2319468/1‑71 (MQ=255)
                                               gCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCAcc                      <  1:5032371/69‑1 (MQ=255)
                                                 gATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCtt                                   <  1:7166137/54‑1 (MQ=255)
                                                 gATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCg                  <  1:7327587/71‑1 (MQ=255)
                                                  aTTGTCTGTGCAGCTATCTATAGCTTCGCGCCGAAAGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCgg                 <  1:870073/71‑1 (MQ=255)
                                                   ttGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCgg                 <  1:4676444/70‑1 (MQ=255)
                                                   ttGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGt                >  1:2655277/1‑71 (MQ=255)
                                                    tGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGt                                            >  1:1507183/1‑42 (MQ=255)
                                                    tGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCt                                    >  1:2189711/1‑50 (MQ=255)
                                                        tgtgCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGGACCATCGGTCAGCt           >  1:5339531/1‑71 (MQ=255)
                                                        tgtgCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATAGGTCAGCt           >  1:1651803/1‑71 (MQ=255)
                                                        tgtgCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGAGGCTTTATGTGGGGCACCATCGGTCAGCt           >  1:6977710/1‑71 (MQ=255)
                                                          tgCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCt           <  1:5223360/69‑1 (MQ=255)
                                                          tgCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCt           <  1:3481887/69‑1 (MQ=255)
                                                          tgCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGa         >  1:6267658/1‑71 (MQ=255)
                                                            cAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGAtt       >  1:7233294/1‑71 (MQ=255)
                                                            cAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGAtt       >  1:7242223/1‑71 (MQ=255)
                                                            cAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGAtt       <  1:5746569/71‑1 (MQ=255)
                                                              gCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGc     <  1:5150504/71‑1 (MQ=255)
                                                              gCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGc     <  1:5399210/71‑1 (MQ=255)
                                                              gCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGc     <  1:312933/71‑1 (MQ=255)
                                                                 atctatAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGt  >  1:6951989/1‑71 (MQ=255)
                                                                 atctatAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGt  >  1:2571973/1‑71 (MQ=255)
                                                                  tctatAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGt  <  1:2339575/70‑1 (MQ=255)
                                                                  tctatAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGt  <  1:5947094/70‑1 (MQ=255)
                                                                   ctatAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTggg                           <  1:3747337/44‑1 (MQ=255)
                                                                   |                                                                    
AAGCATTTAACGGCATTTCCCAGCGCCTGATCCCAGGTGCGGTTCTGGCGATTGACTGTGCAGCTATCTATAGCTTCGCGCCGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGT  >  W3110S.gb/4425548‑4425683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: