| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,534,174 | G→A | 5.9% | A251V (GCA→GTA) | sgcC ← | predicted phosphotransferase enzyme IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,534,174 | 0 | G | A | 5.9% | 447.8 / 11.2 | 136 | A251V (GCA→GTA) | sgcC | predicted phosphotransferase enzyme IIC component |
| Reads supporting (aligned to +/- strand): ref base G (69/59); new base A (6/2); total (75/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
Only 100 of 138 total aligned reads displayed.
GCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGAC > W3110S.gb/4534105‑4534243 | gCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTg < 1:6513419/70‑1 (MQ=255) ccGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTACCgt > 1:6223171/1‑70 (MQ=255) cGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCa < 1:5719589/71‑1 (MQ=255) cGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCa < 1:1973294/71‑1 (MQ=255) cGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTACCgtt > 1:5079363/1‑69 (MQ=255) agaTGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGt > 1:759664/1‑70 (MQ=255) gaTGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGcc > 1:1160660/1‑61 (MQ=255) gaTGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCg > 1:461552/1‑62 (MQ=255) gaTGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCg > 1:3175255/1‑62 (MQ=255) aTGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAat > 1:4521770/1‑71 (MQ=255) tGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTg < 1:267031/57‑1 (MQ=255) tGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatc < 1:2327186/71‑1 (MQ=255) ggCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGcc > 1:1230919/1‑57 (MQ=255) ggCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGcc > 1:348957/1‑57 (MQ=255) ggCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGcc > 1:1205075/1‑57 (MQ=255) ggCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcat < 1:5686698/71‑1 (MQ=255) cagcagGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcat > 1:3297438/1‑69 (MQ=255) cagcagGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcat > 1:1040488/1‑69 (MQ=255) cagcagGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcat > 1:3625694/1‑69 (MQ=255) cagcagGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcat > 1:6991374/1‑69 (MQ=255) cagcagGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcat > 1:218309/1‑69 (MQ=255) agcagGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcat > 1:3580322/1‑68 (MQ=255) agcagGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcat > 1:3227547/1‑68 (MQ=255) agcagGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcat > 1:6200510/1‑68 (MQ=255) ggCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTaa > 1:550066/1‑60 (MQ=255) ggCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTg < 1:5223121/71‑1 (MQ=255) ccTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGc < 1:2480681/46‑1 (MQ=255) ccTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGc < 1:5863288/46‑1 (MQ=255) cTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGc < 1:5455526/48‑1 (MQ=255) cTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGc < 1:3959226/48‑1 (MQ=255) cTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgcg > 1:765875/1‑70 (MQ=255) cTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgcg > 1:146320/1‑70 (MQ=255) tCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTa < 1:6907201/54‑1 (MQ=255) tCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTa < 1:1216249/54‑1 (MQ=255) tCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTa < 1:3872637/54‑1 (MQ=255) tCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcataa > 1:791411/1‑59 (MQ=255) caacaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgcg > 1:6448157/1‑68 (MQ=255) caacaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCaa > 1:358950/1‑71 (MQ=255) caacaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCaa > 1:7588284/1‑71 (MQ=255) caacaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCaa > 1:2125743/1‑71 (MQ=255) aacaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCaa < 1:4454740/70‑1 (MQ=255) aacaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCa < 1:2831873/69‑1 (MQ=255) aacaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAAc > 1:6181629/1‑71 (MQ=255) acaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTg < 1:6933/63‑1 (MQ=255) acaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTg < 1:6658210/63‑1 (MQ=255) acaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAAcc > 1:5169313/1‑71 (MQ=255) acaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTACCGTTGCGGCAatcat > 1:246740/1‑56 (MQ=21) acaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTACCGTTGCGGCAatca > 1:6491697/1‑55 (MQ=21) caaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTa < 1:79367/50‑1 (MQ=255) caaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTa < 1:240494/50‑1 (MQ=255) caaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAAc > 1:2744211/1‑69 (MQ=255) aaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCtt > 1:4743323/1‑71 (MQ=255) aTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCaa > 1:2367294/1‑66 (MQ=255) aTCAGGCGGATCATGCGCGGGAACAACACCATGATTACCGTTGCGGCAatca < 1:7791861/52‑1 (MQ=21) tCAGGCGGATCATGCGCGGGAACAACAGCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTa < 1:7732718/71‑1 (MQ=255) tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgc > 1:4504541/1‑61 (MQ=255) tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgc > 1:7276291/1‑61 (MQ=255) tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgc > 1:4866187/1‑61 (MQ=255) tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgc > 1:3862856/1‑61 (MQ=255) tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgc > 1:1868629/1‑61 (MQ=255) tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCACCCTTTa < 1:1014473/71‑1 (MQ=255) tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCttt > 1:3719538/1‑70 (MQ=255) tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCttt > 1:7438592/1‑70 (MQ=255) tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCttt > 1:7715300/1‑70 (MQ=255) cAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTa > 1:2001768/1‑70 (MQ=255) ggCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAg < 1:6979060/71‑1 (MQ=255) cGGATCATGCGCGGGAACAACACAATGATTGCCGCaa < 1:1500449/37‑1 (MQ=255) ggATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCt > 1:4845516/1‑71 (MQ=255) tCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTg < 1:678860/50‑1 (MQ=255) cATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTc < 1:7760253/69‑1 (MQ=255) cATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTc < 1:7009228/69‑1 (MQ=255) cATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTc < 1:2446179/69‑1 (MQ=255) cATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCAc > 1:7759926/1‑71 (MQ=255) cATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCAc > 1:7467130/1‑71 (MQ=255) aTGCGCGGGAACAACACCATGATTGCCGCAACGGTAAtcatca < 1:2338942/43‑1 (MQ=255) aTGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCAc < 1:2055/70‑1 (MQ=255) tGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCAc > 1:1863965/1‑69 (MQ=255) gcgcGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAg > 1:4617159/1‑71 (MQ=255) cgcgGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgc > 1:2924347/1‑71 (MQ=255) cgcgGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgc > 1:2664481/1‑71 (MQ=255) gggAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgccgc > 1:7358855/1‑71 (MQ=255) gAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCa < 1:3176720/71‑1 (MQ=255) acaacaCCATGATTGCCGCAACGGTAATCATCAGACTGGCTCAACCTTTAAAGCCTTCAc < 1:4067049/60‑1 (MQ=255) acaacaCCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCa > 1:5341008/1‑69 (MQ=255) acacCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGcc > 1:344326/1‑70 (MQ=255) acacCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCa < 1:290395/71‑1 (MQ=255) cacCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCaa < 1:388625/71‑1 (MQ=255) acCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCaa > 1:6883063/1‑70 (MQ=255) ccATGATTGCCGCATCGGTAATCATCAGACTGGCGCAACCTTTAAAGcc < 1:3558685/49‑1 (MQ=255) ccATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgccgcc < 1:2498780/62‑1 (MQ=255) ccATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCaaaa < 1:3942512/71‑1 (MQ=255) cATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCaaaaa < 1:7268501/71‑1 (MQ=255) cATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCaaaaa < 1:6337851/71‑1 (MQ=255) aTGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCaaaa < 1:3350527/69‑1 (MQ=255) tGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATc < 1:367396/71‑1 (MQ=255) aTTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAAt > 1:507936/1‑68 (MQ=255) aTTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCa < 1:1871928/70‑1 (MQ=255) aTTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCa < 1:550969/70‑1 (MQ=255) aTTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAg < 1:4204892/71‑1 (MQ=255) tGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGAc > 1:6325060/1‑71 (MQ=255) | GCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGAC > W3110S.gb/4534105‑4534243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |