BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,563,589	T→A	100%	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,563,589	0	T	A	100.0%	334.3 / NA	109	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (41/68); total (41/68)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 117 total aligned reads displayed.

GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGG  >  W3110S.gb/4563522‑4563655

ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                                 <  1:4631006/71‑1 (MQ=255)

ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                                 <  1:2724717/71‑1 (MQ=255)

ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                                 <  1:2451438/71‑1 (MQ=255)

ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                                 <  1:1932837/71‑1 (MQ=255)

ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                                 <  1:7537678/71‑1 (MQ=255)

ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatt                                                                  >  1:3783734/1‑70 (MQ=255)

 gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                                 >  1:1128646/1‑70 (MQ=255)

  tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                               <  1:2893996/71‑1 (MQ=255)

  tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                               <  1:3838530/71‑1 (MQ=255)

   cGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                              >  1:4208595/1‑71 (MQ=255)

    ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                              >  1:1029412/1‑70 (MQ=255)

    ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                             <  1:7443092/71‑1 (MQ=255)

    ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                             <  1:6222723/71‑1 (MQ=255)

        gcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCg                                                         >  1:7656790/1‑71 (MQ=255)

         cagcTTGCTGATCGGCACATAGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGc                                                        >  1:6944934/1‑71 (MQ=255)

           gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                       <  1:484661/70‑1 (MQ=255)

            cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                     >  1:5385266/1‑71 (MQ=255)

             ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                     >  1:4601769/1‑70 (MQ=255)

             ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                     >  1:5175124/1‑70 (MQ=255)

              tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                   >  1:7392076/1‑71 (MQ=255)

              tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                   >  1:5278378/1‑71 (MQ=255)

              tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                   >  1:388110/1‑71 (MQ=255)

                   aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                                <  1:4793634/69‑1 (MQ=255)

                   aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                                <  1:1213130/69‑1 (MQ=255)

                    tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                              >  1:1461306/1‑70 (MQ=255)

                    tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAac                                             <  1:2848437/71‑1 (MQ=255)

                     cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                            >  1:7000662/1‑71 (MQ=255)

                     cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTCTCGCCCATGTGGAac                                             >  1:3113510/1‑70 (MQ=255)

                      ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac                                           <  1:4292411/71‑1 (MQ=255)

                       gCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                                 <  1:6824241/48‑1 (MQ=255)

                       gCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg                                          <  1:1513169/71‑1 (MQ=255)

                        cacaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                         <  1:4069256/71‑1 (MQ=255)

                        cacaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                         <  1:2372565/71‑1 (MQ=255)

                        cacaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                         <  1:7074730/71‑1 (MQ=255)

                         acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                        >  1:3261435/1‑71 (MQ=255)

                           aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                        <  1:589517/69‑1 (MQ=255)

                           aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                        <  1:4788300/69‑1 (MQ=255)

                             cGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACg                                    >  1:3798311/1‑71 (MQ=255)

                             cGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACg                                    >  1:4951573/1‑71 (MQ=255)

                               cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                                  <  1:1668385/71‑1 (MQ=255)

                                aGTTTTCCAGCATGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTg                                       <  1:4192020/65‑1 (MQ=255)

                                 gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                                    <  1:4055573/51‑1 (MQ=255)

                                 gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                                    <  1:7037253/51‑1 (MQ=255)

                                 gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                                    <  1:5815717/51‑1 (MQ=255)

                                 gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                                    <  1:4378216/51‑1 (MQ=255)

                                 gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg                                >  1:2663190/1‑71 (MQ=255)

                                  ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                             <  1:6815041/41‑1 (MQ=255)

                                  ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTg                                                            >  1:264260/1‑42 (MQ=255)

                                  ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                                <  1:2459751/54‑1 (MQ=255)

                                  ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg                               >  1:2837948/1‑71 (MQ=255)

                                  ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg                               >  1:264263/1‑71 (MQ=255)

                                   tttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                               >  1:7003825/1‑38 (MQ=255)

                                   tttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt                                                  >  1:4762347/1‑51 (MQ=255)

                                   tttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt                                                  >  1:3411904/1‑51 (MQ=255)

                                   tttCCAGCAGGTCATAAATAGGCTGTAACGCCAATTTACTGTCGCCCAtgt                                                  >  1:136046/1‑51 (MQ=255)

                                   tttCCAGCAGGACATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt                                                  >  1:725951/1‑51 (MQ=255)

                                    ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATTTGGAACACGGTGACGCCa                                 <  1:7570750/67‑1 (MQ=255)

                                    ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                                  <  1:5766156/66‑1 (MQ=255)

                                     tCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCa                                 <  1:1724985/66‑1 (MQ=255)

                                      ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                                >  1:3926104/1‑50 (MQ=255)

                                      ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                            <  1:1639277/70‑1 (MQ=255)

                                         gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg                        >  1:7696194/1‑71 (MQ=255)

                                           agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                      <  1:7843546/71‑1 (MQ=255)

                                           agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                      <  1:7852403/71‑1 (MQ=255)

                                           agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                      <  1:2988742/71‑1 (MQ=255)

                                           agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                      <  1:3964919/71‑1 (MQ=255)

                                             gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                     <  1:6986234/38‑1 (MQ=255)

                                             gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                     <  1:5596549/38‑1 (MQ=255)

                                             gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg                                <  1:4478961/59‑1 (MQ=255)

                                             gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg                                <  1:2382021/59‑1 (MQ=255)

                                             gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg                                <  1:1787765/59‑1 (MQ=255)

                                             gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa                           <  1:1369977/64‑1 (MQ=255)

                                             gTCATAAATAGGCTGTAACGCCATTTTACTGTCGACCATGTGGAACACGGTGACGCCAg                                <  1:5972355/59‑1 (MQ=255)

                                              tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                   <  1:3643489/71‑1 (MQ=255)

                                              gcATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                   <  1:234948/70‑1 (MQ=255)

                                               cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                      >  1:184270/1‑51 (MQ=255)

                                                aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc                  >  1:4732119/1‑70 (MQ=255)

                                                aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc                  <  1:4371901/70‑1 (MQ=255)

                                                 tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                                  <  1:1435575/53‑1 (MQ=255)

                                                 tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa                 <  1:1945130/70‑1 (MQ=255)

                                                   aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa               <  1:3166561/70‑1 (MQ=255)

                                                    aTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa               <  1:7405460/69‑1 (MQ=255)

                                                     tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                            <  1:2624392/39‑1 (MQ=255)

                                                     tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                            <  1:1151056/39‑1 (MQ=255)

                                                     tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                            <  1:6497197/39‑1 (MQ=255)

                                                     tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                            <  1:6252629/39‑1 (MQ=255)

                                                     tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                            <  1:5109249/39‑1 (MQ=255)

                                                       ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg                                          >  1:5031529/1‑39 (MQ=255)

                                                         cTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGCTTACCGCCGAGCAAACCGc           <  1:3457401/68‑1 (MQ=255)

                                                            tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa                 <  1:1469457/59‑1 (MQ=255)

                                                            tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCaa        <  1:4108350/68‑1 (MQ=255)

                                                            tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc     >  1:2934701/1‑71 (MQ=255)

                                                            tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc     <  1:6777860/71‑1 (MQ=255)

                                                            tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc     <  1:7234640/71‑1 (MQ=255)

                                                            tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcg      <  1:3687977/70‑1 (MQ=255)

                                                            tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc       <  1:2503544/69‑1 (MQ=255)

                                                              aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGt                              >  1:4624864/1‑44 (MQ=255)

                                                              aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg   <  1:4357413/71‑1 (MQ=255)

                                                               cGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAcc             <  1:1501513/60‑1 (MQ=255)

                                                                gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCggg  >  1:2327739/1‑70 (MQ=255)

GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGG  >  W3110S.gb/4563522‑4563655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`