| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 236,107 | A→C | 78.5% | E14A (GAA→GCA) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 236,107 | 0 | A | C | 78.5% | 1762.2 / 282.1 | 650 | E14A (GAA→GCA) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base A (66/74); new base C (266/243); total (333/317) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
Only 100 of 678 total aligned reads displayed.
AACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTG > W3110S.gb/236039‑236175 | caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:5468908‑M2/6‑71 (MQ=255) cGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAacc < 1:6610053/71‑1 (MQ=255) ctCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca > 1:2049019‑M2/3‑71 (MQ=255) ctCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca > 1:6059205‑M2/3‑71 (MQ=255) tCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac > 1:300007‑M2/2‑68 (MQ=255) cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc > 1:7026189/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAaccac > 1:5850072/1‑69 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTCCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc < 1:5887597/70‑1 (MQ=255) gTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg > 1:7107839/1‑70 (MQ=255) cTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTAt > 1:2539827/1‑71 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:1535168/70‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:7627486/70‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGa < 1:6349245/70‑1 (MQ=255) aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac < 1:6685854/57‑1 (MQ=255) tAAATTACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:6086221/71‑1 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAcc < 1:2257000/70‑1 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca > 1:3867051/1‑57 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:4133361/1‑68 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:6262299/1‑68 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGAACGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:1764831/1‑68 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt > 1:1901778/1‑71 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCg > 1:6771451/1‑58 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt > 1:4748996/1‑71 (MQ=255) gACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg > 1:7282953/1‑71 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg < 1:280832/70‑1 (MQ=255) ccGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGt < 1:2956636/71‑1 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc < 1:5159107/61‑1 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa < 1:6900451/45‑1 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTg < 1:6968637/71‑1 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg > 1:7891808/1‑71 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg < 1:142722/71‑1 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTa > 1:4702941/1‑52 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa > 1:5648926/1‑71 (MQ=255) gAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca > 1:287350/1‑44 (MQ=255) gAGCACTGCAATTACACGCCAGATCGTTCTCG‑‑CCCGCAACCACCgg > 1:4050282/1‑46 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:6445459/1‑50 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:5308080/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:7060756/71‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:939987/1‑70 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:881490/71‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:842764/71‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCTCACTATGa < 1:3023026/71‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:673104/1‑69 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTgg > 1:4211894/1‑58 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa < 1:2009642/71‑1 (MQ=255) tGCTATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:4655486/1‑41 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTa < 1:6208072/44‑1 (MQ=38) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg < 1:393394/46‑1 (MQ=39) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa < 1:1814706/63‑1 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:6373281/1‑71 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:3531505/1‑71 (MQ=255) cAATTACACGCGAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:398432/1‑71 (MQ=255) aTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:1253268/1‑45 (MQ=38) aTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGcc > 1:4280058/1‑70 (MQ=255) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca < 1:745732/71‑1 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:4971218/42‑1 (MQ=38) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:3169465/62‑1 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:1317819/1‑63 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:5103860/40‑1 (MQ=38) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcgc > 1:2678465/1‑55 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:7207730/1‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:3673080/1‑69 (MQ=255) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc < 1:4405167/39‑1 (MQ=37) gCCAGATCGTTTTCGCTACCGCAACCACCGGTATGAACCAGAtt < 1:1840905/44‑1 (MQ=25) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAcc > 1:1083816/1‑39 (MQ=37) gCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt > 1:3440712/1‑70 (MQ=255) aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAATATCAtt < 1:3354295/71‑1 (MQ=255) aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt < 1:7567058/71‑1 (MQ=255) gATCGTTCTCGCTACCGCAACCACCTGTATGAACCAGATTGGTGCGCACTATg < 1:3231219/53‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:7765007/35‑1 (MQ=37) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:5754617/35‑1 (MQ=37) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg < 1:2442682/45‑1 (MQ=38) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:4486571/65‑1 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:34939/1‑67 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGa > 1:1134130/1‑51 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:4563005/1‑67 (MQ=255) gTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgaga < 1:2729840/70‑1 (MQ=255) gTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt > 1:5228301/1‑71 (MQ=255) ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:5036041/56‑1 (MQ=255) tctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa > 1:2402570/1‑42 (MQ=38) tctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg > 1:602601/1‑71 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg > 1:2628446/1‑71 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg < 1:7331915/70‑1 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg < 1:3586549/70‑1 (MQ=255) ctcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg < 1:1607871/70‑1 (MQ=255) tcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt < 1:3102823/67‑1 (MQ=255) cgctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTg > 1:1953594/4‑71 (MQ=255) gctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGc > 1:4316518/3‑71 (MQ=255) gctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGc > 1:6139908/3‑71 (MQ=255) ctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGcc > 1:20995/2‑71 (MQ=255) aTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2291982/42‑1 (MQ=255) aTACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt < 1:3727679/64‑1 (MQ=255) tACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa < 1:2169160/43‑1 (MQ=255) aCCGCAACCACCGGTATGAACGAGATTGGTGCGCACTATGa < 1:2338826/41‑1 (MQ=38) aCCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAtt > 1:5376795/1‑63 (MQ=255) ccGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa > 1:100279/1‑40 (MQ=255) ccGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgag < 1:3781144/59‑1 (MQ=255) cGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa < 1:4851924/40‑1 (MQ=255) cGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTg < 1:1706442/71‑1 (MQ=255) cGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCAACAAGATCAtt > 1:5562867/1‑55 (MQ=255) | AACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTG > W3110S.gb/236039‑236175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |